载基因磁性脂质微泡的制备及对人HepG2细胞的生长抑制作用研究

目的探讨自制载基因磁性脂质微泡的制备方法及该微泡在联合磁感应热疗和基因治疗下对人HepG2细胞的生长抑制作用。方法采用机械振荡法制备载基因磁性脂质微泡。对磁性脂质微泡进行表征分析及检测Fe3O4磁性脂质微泡的升温情况。利用pEGFP-C1观察磁性脂质微泡介导外源基因转染的效率。MTT法,流式细胞仪评价载基因磁性脂质微泡对人HepG2细胞生长情况的影响。结果载基因磁性脂质微泡粒径小,性质稳定,具有良好的磁响应性能,可将外源WtP53基因转染至HepG2细胞系并高效表达,在磁场作用下具有升温恒温能力,在联合磁感应热疗和基因治疗下对人HepG2细胞有生长抑制作用。结论载基因磁性脂质微泡为肿瘤的联合治疗开辟新的思路。

Clinical evaluation of thyroid cancer in sound pulsed radiographic imaging and correlation analysis of OPN and OPN mRNA expression

Objective:To investigate the clinical assessment of thyroid carcinoma in acoustic pulse radiation imaging and the correlation analysis of osteopontin expression.Methods: A total of 71 lesions of 60 patients with thyroid carcinoma (TC) in Hainan were selected. All patients were graded by TI-RADS grading and confirmed by pathology after operation. The expression of OPN was detected by immunohistochemical S-P method. The expression of OPN mRNA was detected by fluorescence quantitative polymerase chain reaction. The correlation between OPN expression and acoustic pulse radiation imaging was analyzed.Results:(1) 60 patients (93.33%) with primary TC and 4 (6.67%) patients with secondary TC were found in 60 patients with TC. Thirty-seven patients (61.67% according to the pathological features, 32 cases (53.33%) had papillary TC, diffuse sclerosis 19 cases of diffuse sclerosis PTC (31.67%), follicular carcinoma in 9 cases (15.00%). (2) The expression of OPN mRNA in 37 cases of calcified TC was significantly higher than that in non-calcified TC, and the increase of OPN expression was in direct proportion to the distribution of gritty calcification.Conclusions: The distribution of calcified microsomes in TC was positively correlated with the expression of OPN, suggesting that enhanced OPN expression may be related to the formation of psammoma bodies, the increased hardness of nodules and the increased malignancy. Acoustic pulse radiation imaging technology for early diagnosis of TC and prognosis of TC was of great significance.

Quantitatively evaluation on pelvic floor dysfunction by virtual touch tissue imaging quantification shear wave elastography

Objective:To investigate the value of(VTIQ)shear wave elastography in the diagnosis of pelvic floor dysfunction.Methods:20 patients with pelvic floor dysfunction and 40 healthy volunteers were enrolled in and devided into the pelvic floor dysfunction(PFD)group and the control group.20 cases in the normal non-fertile(NNF)group and the normal fertile(NF)group respectively were including in the control group.The Young's modulus of puborectalis(PR)in three groups was measured by virtual touch tissue imaging quantification(VTIQ)at different states.The Young's modulus of PR and their differences in the three groups and between groups were compared and calculated.Receiver-operating characteristic curve(ROC)analyses were performed to assess the diagnostic value of VTIQ,and the area under the curve(AUC)was compared.Results:Young's modulus of PR at resting-state,maximun rectal state and Vasalva state in NNF group and NF group were statistical significance(all P<0.05).Young's modulus of maximun rectal state was higher than that of resting-state and Vasalva state in PFD group(P<0.05).There was no significant difference in Young's modulus between the resting-state and Vasalva state in the PFD group(P>0.05).Difference of PR Young's modulus between resting status and maximun rectal state and that between resting status and Vasalva state were of statistical significance among the three groups(all P<0.05).ROC curves analyses indicated that the AUC,cut-off value,sensitivity,specificity and Youden index of the difference between the Young's modulus of resting status and maximun rectal state measured by VTIQ in control group and PFD group were 0.788,35.45kPa,91.67%,57.14%,0.488,espectively.The AUC,cut-off value,sensitivity,specificity and Youden index of the difference between the Young's modulus of resting status and Vasalva state measured by VTIQ in control group and PFD group were 0.799,14.00kPa,63.89%,85.71%,0.496,respectively.There was no significant difference in AUC between the two groups(Z=0.130,P>0.05).Conclusion:VTIQ technology is an effective method to quantitatively assess the biomechanical properties of PR.The effect of labor on the puborectalis muscle was not significant.The increase of Young's modulus of passive stretching in vasalva state was not significant in patients with pelvic floor dysfunction.The difference between resting status and maximun rectal state and difference between resting status and Vasalva state is more advantageous to the diagnosis of pelvic floor dysfunction disorder.

Preparation of multi-mode probe loaded with cisplatin and Fe3O4 folic acid and its targeting abilityin vitro

Objective:To prepare a encapsulated liquid fluorocarbon,multimodal nanoscale ultrasonic molecular probes capable of carrying Cisplatin(CDDP)、Fe3O4 and folic acid molecular targeting,study on its basic characteristics and in vitro targeting ability.Methods:The PLGA-Fa、Fe3O4、CDDP and PFP were dissolved in organic solvents in a certain proportion after the folic acid(Fa)molecule which in the surFace of PLGA was attached to polyethylene glycol imide,prepare nanoscale targeted multimodal ultrasound contrast agent PLGA-Fa/Fe3O4/CDDP/PFP,containing Fe3O4、CDDP、PFP by double creaming methods,the basic properties of it,encapsulation efficiency and drug loading of nanometer particle were observed and measured to optimize the optimal dosage of CDDP;the PFFCP and PFCP were acted on in vitro human nasopharyngeal carcinoma(NPC)cell line HNE-1(high expression of Fa receptor),observe and compare its targeting.Results:Nanoscale targeted multimodal ultrasound contrast agentPLGA-Fa/Fe3O4/CDDP/PFP containing Fe3O4、CDDP、PFP was successfully prepared,the size of the nanometer particle is uniform,the shape is round,the dispersion is good,the diameter distribution is 204nm,and the average electric potential is-15Mv.The nanometer particle could bind to human NPC cell line HNE-1 cells with high expression of Fa receptor,but the nontarget nanometer particle binding of human NPC cell line HNE-1 cells are not obvious.Conclusion:In this study,nanoscale targeted multimodal ultrasound contrast agent PLGA-Fa/Fe3O4/CDDP/PFP containingFe3O4、CDDP、PFP was successfully prepared,and the optimal dose of CDDP was optimized.The nanoparticle have obvious targeting to human NPC cell line HNE-1 cells.

Ultrasonic findings of papillary thyroid carcinoma and its relationship with osteopontin expression

Objective: The ultrasonic findings of papillary thyroid carcinoma were analyzed by acoustic power pulse imaging, and the relationship between the expression of osteopontin and the expression of osteopontin was analyzed. Methods: Using color Doppler ultrasound in patients with lesions, morphology, internal echo, calcification and blood flow of the situation, and then switch to ARFI VTI mode and VTQ mode, VTI were measured with two-dimensional area and area ratio of VTQ mode SWV;osteopontin expression was detected in two groups of patients with thyroid tissues. Analysis of difference between the two the group index by using SPSS18.0 software, Pearson linear correlation method is used to analyze the area ratio and SWV value of thyroid tissue expression correlated with OPN. Results: The shape of papillary thyroid carcinoma nodules showed that the boundary was irregular and the shape of the nodules was burr. The shape of benign nodules was more than 1 with low echo in the interior. The shape of benign nodules showed clear boundary and middle hyperechoic in the interior. No calcification occurred in most lesions in that observation group, the area ratio in VTI mode is obviously higher than that of the control group, the difference was statistically significant (T=7.313, P=0.000), the SWV value in the VTQ model of the observation group was also significantly higher than that in the control group, the difference was statistically significant (t=43.334, P=0.000). The expression of OPNmRNA in the observation group was significantly higher than that of the control group , and the difference was statistically significant (t=8.894, P=0.000). Pearson linear correlation analysis showed that there was a significant positive correlation between OPNmRNA expression in papillary thyroid carcinoma and area ratio in VTI model and SWV course in VTQ model (r=0.235, 0.264;P<0.05). Conclusion: It is important for the diagnosis of papillary thyroid carcinoma to use the technique of acoustic power pulse imaging combined with the detection of OPNmRNA expression. It can be used as a new method for the diagnosis and prognosis of papillary thyroid carcinoma.