Preinduced intestinal HSP70 improves visceral hypersensitivity and abnormal intestinal motility in PI-IBS mouse model

Objective:To investigate the impact of the preinduced intestinal heat shock protein 70(HSP70)on the visceral hypersensitivity and abnormal intestinal motility in a post-infectious irritable bowel syndrome(PI-IBS) mouse model.Methods:Eighty-four female C57BL/6 mice were randomly assigned to four groups:control group(n=21) and induction+PI-IBS group(n=21),PI-IBS group(n=21) and induction group(n=21).The mice in PI-IBS group were infected in vivo with trichinella spiralis by oral administration.The visceral hypersensitivity and intestinal motility were evaluated respectively with abdominal withdrawal reflex and colon transportation test.The intestinal HSP70 protein and mRNA level was measured by Western blot and realtime PCR.Meanwhile,the intestinal proinflammatory cytokines IL-10 and TNF-α level was detected by ELISA.Results:Compared with their counterparts in PI-IBS group,the animals in the Induction+PI-IBS group show significantly increased intestinal level of HSP70 and obviously ameliorative clinical tigurcs.including abdominal withdrawal reflex score,intestine transportation time and Bristol scores(P<0.05).Meanwhile,the intestinal post-inflammatory cytokines remarkably changed,including increased IL-10 level and decreased TNF-αlevel(P<0.05).Conclusions:Intestinal IISP70 may play a potential protective role through improving the imbalance between the intestinal post-inflammatory and anti-inflammatory cytokines in PI-IBS.

Study on the role of magnetic resonance enterography in valuating infliximab therapy in small bowel crohn's disease

Objective:To investigate the role of magnetic resonance enterography (MRE) in valuating infliximab's (IFX)curative effect in active small bowel Crohn's Disease (CD).Methods:27 patients diagnosed as active CD were treated with IFX. Before and after the treatment, the patients were scanned with 3.0 T MRE after small intestinal preparation. The imaging characteristic was analyzed and the relevance analysis between the imaging performance and the clinical score were investigated.Results: (1) Before IFX treatment, MRE could clearly detect the intestinal wall and extra luminal lesion in active CD, including intestinal wall multiple segmental thickening and abnormal enhancement, irregular signaling, comb sign, and intestinal stricture or dilation;(2) After IFX treatment, MRE show that intestinal wall thinning and the dilated bowel lumen were improved, and the comb sign disappeared;(3) During artery phase, the signal of the intestinal lesion was improved from high signal or equal signal before the IFX treatment to equal signal or low signal after treatment (P<0.05). During vein phase, there was no significant change of lesion signal before and after treatment (P>0.05).4. the changes of imaging performance were consistant with that of the clinical score (P>0.05). Conclusions: MRE possesses clinical value in evaluating the IFX curative effect in active small bowel CD.