Ulcerative colitis flair induced by mesalamine suppositories hypersensitivity

Mesalamine suppositories have been used widely for the treatment of distal ulcerative colitis and considered to be safer than systemic administration for its limited systemic absorption.However,previous studies have shown that mesalamine suppository occasionally causes severe hypersensitivity reactions including fever,rashes,colitis exacerbation and acute eosinophilic pneumonia.Here we present a 25-year-old woman with ulcerative colitis with bloody diarrhea accompanied by abdominal pain and fever which were aggravated after introduction of mesalamine suppositories.In light of symptom exacerbation of ulcerative colitis,increased inflammatory injury of colon mucosa shown by colonoscopy and elevated peripheral eosinophil count after mesalamine suppositories administration,and the Naranjo algorithm score of 10,the possibility of hypersensitivity reaction to mesalamine suppositories should be considered,warning us to be aware of this potential reaction after administration of mesalamine formulations even if it is the suppositories.

Correlation of NLRP3 polymorphism with inflammasome activity and endothelial damage in patients with acute coronary syndrome

Objective:To study the correlation of Nod-like receptor protein 3 (NLRP3) polymorphism with inflammasome activity and endothelial damage in patients with acute coronary syndrome. Methods:Patients diagnosed with acute coronary syndrome and stable angina pectoris in Mianyang Central Hospital between May 2013 and August 2016 were selected and included in ACS group and SAP group respectively, and healthy volunteers who received physical examination during the same period were selected as control group. Peripheral blood was collected to detect NLRP3 gene rs10754558 loci polymorphism, and serum was separated to determine inflammasome activity indexes and endothelial injury indexes.Results:NLRP3 gene GG genotype and GC genotype constituent ratio of ACS group were significantly higher than those of SAP group and control group while CC genotype constituent ratio was significantly lower than that of SAP group and control group, and serum IL-1β, IL-18, E-selectin, vWF and ET-1 levels were significantly higher than those of SAP group and control group while serum NO level was significantly lower than that of SAP group and control group;serum IL-1β, IL-18, E-selectin, vWF and ET-1 levels in ACS patients with GG genotype and GC genotype were significantly higher than those in patients with CC genotype while NO levels were significantly lower than those in patients with CC genotype, and serum IL-1β, IL-18, E-selectin, vWF and ET-1 levels in ACS patients with GG genotype were significantly higher than those in patients with GC genotype while NO level was significantly lower than that in patients with GC genotype.Conclusions: The increased NLRP3 gene rs10754558 loci alleles G in patients with ACS will increase inflammasome activity and endothelial injury.

Mechanism of Chinese herbal formula QHF against breast cancer MCF-7 cells invasion and migration

Objective： To investigate the effects of Chinese herbal formula Qinghuofu（QHF） on the migration and invasion of breast cancer MCF-7 cells and its possible molecular mechanisms, thereby providing a theoretical basis to find effective anti-cancer medicine and therapeutic targets for the treatment of anti-migration and anti-invasion of breast cancer.Methods： Breast cancer MCF-7 cells were treated with different QHF and other different reagents, CCK8 assay was used to detect the influence of the reagents on the proliferation of MCF-7 cells; Scrape migration and Transwell assay were used to quantitatively determine the migration and invasion effects of QHF and hepatocyte growth factor（HGF） on the MCF-7 cells. Subsequently, the c-Met inhibitor and its downstream ERK and PI3 K inhibitors were used to investigate the relationship between the migration and invasion of MCF-7 cells, as well as its downstream MAPK/ERK and PI3 K/Akt signaling pathways. The expression levels of HGF, c-Met, ERK, p-Akt, p-c-Met, p-ERK, p-Akt, MMP2, MMP9, and VEGF in breast cancer MCF-7 cells treated with QHF and other reagents were also examined.Results： The result indicated that formula QHF not only significantly inhibited the proliferation of MCF-7 cells, but also significantly suppressed the effects of HGF（40 ng/m L） on the proliferation and movement of MCF-7 cells, reducing the ability of the cells to invade and migrate. Western blot analysis indicated that QHF and c-Met inhibitor significantly decreased the expression of p-c-Met, p-ERK1, p-ERK2, p-Akt, MMP-2, MMP-9, and VEGF, while HGF significantly increased the expression of p-c-Met in MCF-7 cells; c-Met downstream ERK and PI3 K inhibitors also significantly decreased the expression of MMP-2, MMP-9, and VEGF in MCF-7 cells; But the difference among c-Met, PI3 K, ERK, and QHF group were not statistically significant.Conclusion： QHF can prevent the proliferation, migration, and invasion of MCF-7 cells by inhibiting the HGF/c-Met and its downstream PI3 K/Akt and MAPK/ERK signaling pathways; Thereby down-regulating the expression of HGF, p-Met, p-ERK1, p-ERK2, p-Akt, MMP-2, MMP-9, and VEGF.