Objective: The aim of the present study was to analyze the prognostic factors in patients with hepatoblastoma(HB) in our single center and to evaluate periostin(POSTN) expression in HB and its association with clinico...Objective: The aim of the present study was to analyze the prognostic factors in patients with hepatoblastoma(HB) in our single center and to evaluate periostin(POSTN) expression in HB and its association with clinicopathological variables. In addition, the underlying mechanism of how POSTN promotes HB progression was discussed.Methods: POSTN expression was investigated in HB tumors by immunohistochemistry(IHC), immunofluorescence(IF) and Western blot(WB). The association among POSTN expression, clinicopathological features and overall survival(OS) was also evaluated. The migration and adhesion ability of HB cells were measured using chemotaxis and cell-matrix adhesion assays,respectively. Epithelial-mesenchymal transition(EMT)-associated markers and activation of the ERK pathway were detected by WB.Results: HB patients had poor prognosis which displayed lymph node metastasis, vascular invasion, POSTN and vimentin expression. POSTN expression was also associated with lymph node metastasis. Furthermore, overexpressed POSTN promoted migration and the adhesive ability of HB cells in vitro. In addition, we demonstrated that POSTN activated the MAPK/ERK pathway, upregulated the expression of Snail and decreased the expression of OVOL2. Finally, POSTN promoted the expression of EMT-associated markers.Conclusions: POSTN might modulate EMT via the ERK signaling pathway, thereby promoting cellular migration and invasion.Our study also suggests that POSTN may serve as a therapeutic biomarker in HB patients.展开更多
Objective:To analyze the correlation between ultrasonographic findings and Lysholm scores in knee osteoarthritis.Methods:This cross-sectional observational study included 77 cases of knee osteoarthritis examined from ...Objective:To analyze the correlation between ultrasonographic findings and Lysholm scores in knee osteoarthritis.Methods:This cross-sectional observational study included 77 cases of knee osteoarthritis examined from February to December in 2015.There were 66 females and 11 males,with an average age of 57.39 (6.41) years,single or bilateral knee pain and limitation of mobility,and an average course of disease of 13.60 (3.00) months.Multiple regression equations were applied in a linear regression analysis to assess the correlation between ultrasound findings and Lysholm scores.Results:The ultrasonographic findings of joint effusion,synovial hypertrophy,protrusion of the medial meniscus (MMP),and inflammation of subpatellar fat pad were significantly correlated with the Lysholm scores (all P <.05).In contrast,there was no association between the ultrasonographic findings of Baker's cyst or patellar ligament lesion and the Lysholm scores (both P >.05).Conclusion:Musculoskeletal ultrasound can better detect the pathological changes of soft tissue in the knee,mainly including synovial hypertrophy,joint effusion,and MMP in comparison with those by plain radiology.After statistical analysis,it is indicated that ultrasonographic findings of synovial hypertrophy was possibly associated with joint locking,joint effusion with supporting,MMP with squatting,and inflammation of subpatellar fat pad with squatting and locking.展开更多
基金supported by grants from Key Project of Tianjin Natural Science Foundation (Grant No. 18JCZDJC35200)The Science & Technology Development Fund of Tianjin Education Commission for Higher Education (Grant No. 2017KJ202)
文摘Objective: The aim of the present study was to analyze the prognostic factors in patients with hepatoblastoma(HB) in our single center and to evaluate periostin(POSTN) expression in HB and its association with clinicopathological variables. In addition, the underlying mechanism of how POSTN promotes HB progression was discussed.Methods: POSTN expression was investigated in HB tumors by immunohistochemistry(IHC), immunofluorescence(IF) and Western blot(WB). The association among POSTN expression, clinicopathological features and overall survival(OS) was also evaluated. The migration and adhesion ability of HB cells were measured using chemotaxis and cell-matrix adhesion assays,respectively. Epithelial-mesenchymal transition(EMT)-associated markers and activation of the ERK pathway were detected by WB.Results: HB patients had poor prognosis which displayed lymph node metastasis, vascular invasion, POSTN and vimentin expression. POSTN expression was also associated with lymph node metastasis. Furthermore, overexpressed POSTN promoted migration and the adhesive ability of HB cells in vitro. In addition, we demonstrated that POSTN activated the MAPK/ERK pathway, upregulated the expression of Snail and decreased the expression of OVOL2. Finally, POSTN promoted the expression of EMT-associated markers.Conclusions: POSTN might modulate EMT via the ERK signaling pathway, thereby promoting cellular migration and invasion.Our study also suggests that POSTN may serve as a therapeutic biomarker in HB patients.
文摘Objective:To analyze the correlation between ultrasonographic findings and Lysholm scores in knee osteoarthritis.Methods:This cross-sectional observational study included 77 cases of knee osteoarthritis examined from February to December in 2015.There were 66 females and 11 males,with an average age of 57.39 (6.41) years,single or bilateral knee pain and limitation of mobility,and an average course of disease of 13.60 (3.00) months.Multiple regression equations were applied in a linear regression analysis to assess the correlation between ultrasound findings and Lysholm scores.Results:The ultrasonographic findings of joint effusion,synovial hypertrophy,protrusion of the medial meniscus (MMP),and inflammation of subpatellar fat pad were significantly correlated with the Lysholm scores (all P <.05).In contrast,there was no association between the ultrasonographic findings of Baker's cyst or patellar ligament lesion and the Lysholm scores (both P >.05).Conclusion:Musculoskeletal ultrasound can better detect the pathological changes of soft tissue in the knee,mainly including synovial hypertrophy,joint effusion,and MMP in comparison with those by plain radiology.After statistical analysis,it is indicated that ultrasonographic findings of synovial hypertrophy was possibly associated with joint locking,joint effusion with supporting,MMP with squatting,and inflammation of subpatellar fat pad with squatting and locking.