Loquat(Eriobotrya japonica)is a subtropical tree that bears fruit that ripens during late spring.Fruit size is one of the dominant factors inhibiting the large-scale production of this fruit crop.To date,little is kno...Loquat(Eriobotrya japonica)is a subtropical tree that bears fruit that ripens during late spring.Fruit size is one of the dominant factors inhibiting the large-scale production of this fruit crop.To date,little is known about fruit size regulation.In this study,we first discovered that cell size is more important to fruit size than cell number in loquat and that the expression of the EjBZR1 gene is negatively correlated with cell and fruit size.Virus-induced gene silencing(VIGS)of EjBZR1 led to larger cells and fruits in loquat,while its overexpression reduced cell and plant size in Arabidopsis.Moreover,both the suppression and overexpression of EjBZR1 inhibited the expression of brassinosteroid(BR)biosynthesis genes,especially that of EjCYP90A.Further experiments indicated that EjCYP90A,a cytochrome P450 gene,is a fruit growth activator,while EjBZR1 binds to the BRRE(CGTGTG)motif of the EjCYP90A promoter to repress its expression and fruit cell enlargement.Overall,our results demonstrate a possible pathway by which EjBZR1 directly targets EjCYP90A and thereby affects BR biosynthesis,which influences cell expansion and,consequently,fruit size.These findings help to elucidate the molecular functions of BZR1 in fruit growth and thus highlight a useful genetic improvement that can lead to increased crop yields by repressing gene expression.展开更多
A composite transparent YAG/Yb:YAG/YAG ceramic was prepared by a non-aqueous tape-casting method. An optical transmittance of 82% was obtained at visible wavelength and around 1100 nm. A low-threshold, broadband tunab...A composite transparent YAG/Yb:YAG/YAG ceramic was prepared by a non-aqueous tape-casting method. An optical transmittance of 82% was obtained at visible wavelength and around 1100 nm. A low-threshold, broadband tunable continuous-wave(CW) laser at 1031 nm was further demonstrated from the ceramic sample, which was pumped by a974 nm fiber-pigtailed laser diode. The threshold pump power was 0.45 W and the maximum output power was 3.2 W,corresponding to a slope efficiency of 20.4%. By inserting an SF57 prism in the laser cavity, the output wavelength could be tuned continuously from 1021 to 1058 nm.展开更多
基金This work was supported by the National Natural Science Foundation of China(31901973)the Key Areas of Science and Technology Planning Project of Guangdong Province(2018B020202011)+2 种基金the State Key Laboratory for the Conservation and Utilization of Subtropical Agro-Bioresources(201504010028)Fujian Academy of Agricultural Science External Cooperation Program(DWHZ2021-14)the Open Fund of the Key Laboratory of Loquat Germplasm Innovation and Utilization(Putian University)(2018003).
文摘Loquat(Eriobotrya japonica)is a subtropical tree that bears fruit that ripens during late spring.Fruit size is one of the dominant factors inhibiting the large-scale production of this fruit crop.To date,little is known about fruit size regulation.In this study,we first discovered that cell size is more important to fruit size than cell number in loquat and that the expression of the EjBZR1 gene is negatively correlated with cell and fruit size.Virus-induced gene silencing(VIGS)of EjBZR1 led to larger cells and fruits in loquat,while its overexpression reduced cell and plant size in Arabidopsis.Moreover,both the suppression and overexpression of EjBZR1 inhibited the expression of brassinosteroid(BR)biosynthesis genes,especially that of EjCYP90A.Further experiments indicated that EjCYP90A,a cytochrome P450 gene,is a fruit growth activator,while EjBZR1 binds to the BRRE(CGTGTG)motif of the EjCYP90A promoter to repress its expression and fruit cell enlargement.Overall,our results demonstrate a possible pathway by which EjBZR1 directly targets EjCYP90A and thereby affects BR biosynthesis,which influences cell expansion and,consequently,fruit size.These findings help to elucidate the molecular functions of BZR1 in fruit growth and thus highlight a useful genetic improvement that can lead to increased crop yields by repressing gene expression.
基金supported by the Major Program of the National Natural Science Foundation of China (Grant No. 50990301)the Project for Young Scientists Fund of the National Natural Science Foundation of China (Grant Nos. 51002172 and 51302298)
文摘A composite transparent YAG/Yb:YAG/YAG ceramic was prepared by a non-aqueous tape-casting method. An optical transmittance of 82% was obtained at visible wavelength and around 1100 nm. A low-threshold, broadband tunable continuous-wave(CW) laser at 1031 nm was further demonstrated from the ceramic sample, which was pumped by a974 nm fiber-pigtailed laser diode. The threshold pump power was 0.45 W and the maximum output power was 3.2 W,corresponding to a slope efficiency of 20.4%. By inserting an SF57 prism in the laser cavity, the output wavelength could be tuned continuously from 1021 to 1058 nm.
