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Comparison of the emission wavelengths by a single fluorescent dye on in vivo 3-photon imaging of mouse brains 被引量:1
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作者 Ke Wang Wanjian Zhang +8 位作者 xiangquan deng Shen Tong Hui Cheng Mengyuan Qin Lei Zheng Kun Zhao Ruizhan Zhai Zhongqing Jia Ping Qiu 《Journal of Innovative Optical Health Sciences》 SCIE EI CSCD 2023年第6期23-30,共8页
Multiphoton microscopy(MPM)is a powerful imaging technology for brain research.The imaging depth in MPM is partly determined by emission wavelength of fluorescent labels.It has been demonstrated that a longer emission... Multiphoton microscopy(MPM)is a powerful imaging technology for brain research.The imaging depth in MPM is partly determined by emission wavelength of fluorescent labels.It has been demonstrated that a longer emission wavelength is favorable for signal detection as imaging depth increases.However,there has been no comparison with near-infrared(NIR)emission.In order to quantitatively analyze the effect of emission wavelength on 3-photon imaging of mouse brains in vivo,we utilize the same excitation wavelength to excite a single fluorescent dye and simultaneously collect NIR and orange-red emission fluorescence at 828 nm and 620 nm,respectively.Both experimental and simulation results show that as the imaging depth increases,NIR emission decays less than orange-red fluorescent emission.These results show that it is preferable to shift the emission wavelength to NIR to enable more e±cient signal collection deep in the brain. 展开更多
关键词 3-Photon microscopy emission fluorescence FM4-64 Monte Carlo.
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Influence of non-uniform electric field distribution on the atmospheric pressure air dielectric barrier discharge 被引量:2
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作者 Weisheng CUI Shuai ZHAO +3 位作者 Zhengfang QIAN Yiling SUN Mahmoud AL-SALIHI xiangquan deng 《Plasma Science and Technology》 SCIE EI CAS CSCD 2021年第7期78-84,共7页
The dielectric barrier discharge(DBD)in air at atmospheric pressure is not suitable for industrial applications due to its randomly distributed discharge filaments.In this paper,the influence of the electric field dis... The dielectric barrier discharge(DBD)in air at atmospheric pressure is not suitable for industrial applications due to its randomly distributed discharge filaments.In this paper,the influence of the electric field distribution on the uniformity of DBD is theoretically analyzed and experimentally verified.It is found that a certain degree of uneven electric field distributions can control the development of electron avalanches and regulate their transition to streamers in the gap.The discharge phenomena and electrical characteristics prove that an enhanced Townsend discharge can be formed in atmospheric-pressure air with a curved-plate electrode.The spectral analysis further confirms that the gas temperature of the plasma produced by the curved-plate electrode is close to room temperature,which is beneficial for industrial applications.This paper presents the relationship between the electron avalanche transition and the formation of a uniform DBD,which can provide some references for the development and applications of the DBD in the future. 展开更多
关键词 dielectric barrier discharge electric field distribution electron avalanche Townsend discharge
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Aberration correction for multiphoton microscopy using covariance matrix adaptation evolution strategy 被引量:1
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作者 王科 郑磊 +8 位作者 秦梦圆 张万剑 邓想全 仝申 程慧 黄杰 钟金成 张颖娴 邱娉 《Chinese Optics Letters》 SCIE EI CAS CSCD 2023年第5期88-93,共6页
Multiphoton microscopy is the enabling tool for biomedical research,but the aberrations of biological tissues have limited its imaging performance.Adaptive optics(AO)has been developed to partially overcome aberration... Multiphoton microscopy is the enabling tool for biomedical research,but the aberrations of biological tissues have limited its imaging performance.Adaptive optics(AO)has been developed to partially overcome aberration to restore imaging performance.For indirect AO,algorithm is the key to its successful implementation.Here,based on the fact that indirect AO has an analogy to the black-box optimization problem,we successfully apply the covariance matrix adaptation evolution strategy(CMA-ES)used in the latter,to indirect AO in multiphoton microscopy(MPM).Compared with the traditional genetic algorithm(GA),our algorithm has a greater improvement in convergence speed and convergence accuracy,which provides the possibility of realizing real-time dynamic aberration correction for deep in vivo biological tissues. 展开更多
关键词 multiphoton microscopy 1700-nm window adaptive optics covariance matrix adaptation evolution strategy
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