Fermented bamboo shoots(FBS)is a region-specific food widely consumed in Southwestern China,with Lactobacillus as the predominant fermenting bacteria.However,the probiotic potential of Lactobacillus derived from FBS r...Fermented bamboo shoots(FBS)is a region-specific food widely consumed in Southwestern China,with Lactobacillus as the predominant fermenting bacteria.However,the probiotic potential of Lactobacillus derived from FBS reminds largely unexplored,especially for diseases with a low prevalence in areas consuming FBS,namely,inflammatory bowel disease.In this study,Lactiplantibacillus pentosus YQ001 and Lentilactobacillus senioris YQ005 were screening by in vitro probiotic tests to further investigate the probioticlike bioactivity in dextran sulfate sodium(DSS)-induced ulcerative colitis(UC)mouse.They exhibited more positive probiotic effects than Lactobacillus rhamnosus GG in preventing intestinal inflammatory response.The results revealed that both strains improved the abundance of deficient intestinal microbiota in UC mice,including Muribaculaceae and Akkermansia.In the serum metabolome,they modulated the DSS-disturbed levels of metabolites,with significant increment of cinnamic acid.Meanwhile,they reduced the expression levels of interleukin-1β(IL-1β),interleukin-6(IL-6)inflammatory factors and increased zonula occludens-1(ZO-1),Occludin,and cathelicidin-related antimicrobial peptide(CRAMP)in the colon.Consequently,these results demonstrated that Lactobacillus spp.isolates derived from FBS showed promising probiotic activity based on the gut microbiome homeostasis modulation,anti-inflammation and intestinal barrier protection in UC mice.展开更多
Virtual network embedding problem which is NP-hard is a key issue for implementing software-defined network which is brought about by network virtualization. Compared with other studies which focus on designing heuris...Virtual network embedding problem which is NP-hard is a key issue for implementing software-defined network which is brought about by network virtualization. Compared with other studies which focus on designing heuristic algorithms to reduce the hardness of the NP-hard problem we propose a robust VNE algorithm based on component connectivity in large-scale network. We distinguish the different components and embed VN requests onto them respectively. And k-core is applied to identify different VN topologies so that the VN request can be embedded onto its corresponding component. On the other hand, load balancing is also considered in this paper. It could avoid blocked or bottlenecked area of substrate network. Simulation experiments show that compared with other algorithms in large-scale network, acceptance ratio, average revenue and robustness can be obviously improved by our algorithm and average cost can be reduced. It also shows the relationship between the component connectivity including giant component and small components and the performance metrics.展开更多
基金supported by the key project of the Natural Science Foundation of Chongqing(cstc2020jcyj-zdxmX0029)the Science and Technology Research Program of Chongqing Municipal Education Commission(KJQN202100412).
文摘Fermented bamboo shoots(FBS)is a region-specific food widely consumed in Southwestern China,with Lactobacillus as the predominant fermenting bacteria.However,the probiotic potential of Lactobacillus derived from FBS reminds largely unexplored,especially for diseases with a low prevalence in areas consuming FBS,namely,inflammatory bowel disease.In this study,Lactiplantibacillus pentosus YQ001 and Lentilactobacillus senioris YQ005 were screening by in vitro probiotic tests to further investigate the probioticlike bioactivity in dextran sulfate sodium(DSS)-induced ulcerative colitis(UC)mouse.They exhibited more positive probiotic effects than Lactobacillus rhamnosus GG in preventing intestinal inflammatory response.The results revealed that both strains improved the abundance of deficient intestinal microbiota in UC mice,including Muribaculaceae and Akkermansia.In the serum metabolome,they modulated the DSS-disturbed levels of metabolites,with significant increment of cinnamic acid.Meanwhile,they reduced the expression levels of interleukin-1β(IL-1β),interleukin-6(IL-6)inflammatory factors and increased zonula occludens-1(ZO-1),Occludin,and cathelicidin-related antimicrobial peptide(CRAMP)in the colon.Consequently,these results demonstrated that Lactobacillus spp.isolates derived from FBS showed promising probiotic activity based on the gut microbiome homeostasis modulation,anti-inflammation and intestinal barrier protection in UC mice.
基金supported in part by the National Natural Science Foundation of China under Grant No.61471055
文摘Virtual network embedding problem which is NP-hard is a key issue for implementing software-defined network which is brought about by network virtualization. Compared with other studies which focus on designing heuristic algorithms to reduce the hardness of the NP-hard problem we propose a robust VNE algorithm based on component connectivity in large-scale network. We distinguish the different components and embed VN requests onto them respectively. And k-core is applied to identify different VN topologies so that the VN request can be embedded onto its corresponding component. On the other hand, load balancing is also considered in this paper. It could avoid blocked or bottlenecked area of substrate network. Simulation experiments show that compared with other algorithms in large-scale network, acceptance ratio, average revenue and robustness can be obviously improved by our algorithm and average cost can be reduced. It also shows the relationship between the component connectivity including giant component and small components and the performance metrics.
