Rapid Detection of β-lactam Antibiotic Residue in Milk Using Biolayer Interferometry

[ Objective] This study aimed to establish a high-sensitivity method for rapid detection of^-lactam antibiotic residue in milk. [Method] Based on bio- layer interferometry technology, ampicillin-BSA conjugate was fixed on the bottom of APS fiber optic biosensor probe through hydrophobic interaction and bound to 40 mn colloidal gold-labeled/3-1actam antibiotic receptor, to detect β-lactam antibiotics in milk. [ Result] The sensitivity of colloidal gold-labeled BLI method was twice as high as that of immunechromatographic test strip in detection of β-1actam antibiotic residue in milk. Colloidal gold-labeled BLI method exhibited good speci- ficity and had no cross-reaction with 1 000 ng/ml aflatoxin M1, gentamicin, kanamycin, streptomycin, tylosin, chloromycetin and melamine. [Condusion] The colloidal gold-labeled BLI method is not suitable for quantitative detection in actual production due to its small quantitative range in detection of β-lactam antibiot- ics, but it is a simple and rapid qualitative detection method that can be used in rapid detection of β-1actam antibiotic residue in milk.

A uniform solution to the independent set problem through tissue P systems with cell separation

膜计算是自然计算的一个突现的分支，它被结构并且更高在纸巾，器官，和其它细胞的生活细胞，以及组织工作启发目结构。织物 P 系统是在膜计算的框架的大多数调查计算模型的一个班，特别在效率的方面。在一多项式时间产生一个指数的资源，房间分离被合并到如此的系统，因此获得那么与房间分离把织物称为 P 系统。在这个工作，我们利用这个模型的计算效率并且为解决独立集合问题构造如此的织物 P 系统的一个一致家庭，一个著名 NP 完全的问题，一个有效解决方案能被在多项式时间获得。