Atherosclerotic cardiovascular disease and its complications are a high-incidence disease worldwide.Numerous studies have shown that blood flow shear has a huge impact on the function of vascular endothelial cells,and...Atherosclerotic cardiovascular disease and its complications are a high-incidence disease worldwide.Numerous studies have shown that blood flow shear has a huge impact on the function of vascular endothelial cells,and it plays an important role in gene regulation of pro-inflammatory,pro-thrombotic,pro-oxidative stress,and cell permeability.Many impor-tant endothelial cell mechanosensitive genes have been discovered,including KLK10,CCN gene family,NRP2,YAP,TAZ,HIF-1α,NF-kB,FOS,JUN,TFEB,KLF2/KLF4,NRF2,and ID1.Some of them have been intensively studied,whereas the relevant regulatory mechanism of other genes remains unclear.Focusing on these mechanosensitive genes will provide new strategies for therapeutic intervention in atherosclerotic vascular disease.Thus,this article reviews the mechanosensitive genes affecting vascular endothelial cells,including classical pathways and some newly screened genes,and summarizes the latest research progress on their roles in the pathogenesis of atherosclerosis to reveal effective therapeutic targets of drugs and provide new insights foranti-atherosclerosis.展开更多
Purpose:This review aims to explore the history,research hotspots,and emerging trends of drug-eluting stents(DES)in the last two decades from the perspective of structural and temporal dynamics.Methods:Publications on...Purpose:This review aims to explore the history,research hotspots,and emerging trends of drug-eluting stents(DES)in the last two decades from the perspective of structural and temporal dynamics.Methods:Publications on DES were retrieved from WoSCC.The bibliometric tools including CiteSpace and HistCite were used to identify the historical features,the evolution of active topics,and emerging trends on the DES field.Results:In the last 20 years,the field of DES is still in the hot phase and there is a wide range of extensive scientific collaborations.In addition,active topics emerge in different periods,as evidenced by a total of 41 disciplines,511 keywords,and 1377 papers with citation bursts.Keyword clustering anchored five emerging research subfields,namely#0 dual antiplatelet therapy,#3 drug-coated balloon,#4 bifurcation,5#rotational atherectomy,and 6#quantitative flow ratio.The keyword alluvial map shows that the most persistent research concepts in this field are thrombosis,restenosis,etc.,and the emerging keywords are paclitaxel eluting balloon,coated balloon,drug-eluting balloon,etc.There are 7 recent research subfields anchored by reference clustering,namely#2 dual antiplatelet therapy,#4 drug-coated balloon,#5 peripheral artery disease,#8 fractional flow reserve,#10 bioresorbable vascular scaffold,#13 intravascular ultrasound,#14 biodegradable polymer.Conclusion:The findings based on the bibliometric studies provide the current status and trends in DES research and may help researchers to identify hot topics and explore new research directions in this field.展开更多
Multiple signal strategies remarkably improve the accuracy and efficiency of electrochemiluminescence(ECL)immunoassays,but the lack of potential-resolved luminophore pairs and chemical cross talk hinders their develop...Multiple signal strategies remarkably improve the accuracy and efficiency of electrochemiluminescence(ECL)immunoassays,but the lack of potential-resolved luminophore pairs and chemical cross talk hinders their development.In this study,we synthesized a series of gold nanoparticles(AuNPs)/reduced graphene oxide(Au/rGO)composites as adjustable oxygen reduction reaction and oxygen evolution reaction catalysts to promote and modulate tris(2,2′-bipyridine)ruthenium(II)(Ru(bpy)_(3)^(2+))’s multisignal luminescence.With the increase in the diameter of AuNPs(3 to 30 nm),their ability to promote Ru(bpy)_(3)^(2+)’s anodic ECL was first impaired and then strengthened,and cathodic ECL was first enhanced and then weakened.Au/rGOs with medium-small and medium-large AuNP diameters remarkably increased Ru(bpy)_(3)^(2+)’s cathodic and anodic luminescence,respectively.Notably,the stimulation effects of Au/rGOs were superior to those of most existing Ru(bpy)_(3)^(2+)co-reactants.Moreover,we proposed a novel ratiometric immunosensor construction strategy using Ru(bpy)_(3)^(2+)’s luminescence promoter rather than luminophores as tags of antibodies to achieve signal resolution.This method avoids signal cross talk between luminophores and their respective co-reactants,which achieved a good linear range of 10−7 to 10−1 ng/ml and a limit of detection of 0.33 fg/ml for detecting carcinoembryonic antigen.This study addresses the previous scarcity of the macromolecular co-reactants of Ru(bpy)_(3)^(2+),broadening its application in biomaterial detection.Furthermore,the systematic clarification of the detailed mechanisms for converting the potential-resolved luminescence of Ru(bpy)_(3)^(2+)could facilitate an in-depth understanding of the ECL process and should inspire new designs of Ru(bpy)_(3)^(2+)luminescence enhancers or applications of Au/rGOs to other luminophores.This work removes some impediments to the development of multisignal ECL biodetection systems and provides vitality into their widespread applications.展开更多
Dear Editor,Glucocorticoids(GCs)often cause detrimental side effects,including osteoporosis and osteonecrosis,with no specific drugs available to circumvent these problems.1 Cellular senescence plays an essential role...Dear Editor,Glucocorticoids(GCs)often cause detrimental side effects,including osteoporosis and osteonecrosis,with no specific drugs available to circumvent these problems.1 Cellular senescence plays an essential role in bone loss,2,3 where senescent cells release various factors,termed senescence-associated secretory phenotype(SASP),which influences neighboring cells and disrupts normal tissues.2 Glucocorticoids can induce senescence in bone cells in young mice;2 however,the effects of GCs on senescence and SASP during bone metabolism in adult mice are unclear.展开更多
Exosomes are membrane vesicles that exist in pathological or normal cells and are considered to be specifically secreted,contain complex RNAs and proteins,participate in intercellular communication functions,and have ...Exosomes are membrane vesicles that exist in pathological or normal cells and are considered to be specifically secreted,contain complex RNAs and proteins,participate in intercellular communication functions,and have functions in organismal immune response,antigen presentation,cell migration,cell differentiation,and tumor invasion.In recent years,with the explosion of the development of minimally invasive diagnostics,the research of exosomes has been rapidly developed,and the number of articles published in this field has increased dramatically,and many articles summarize the published papers in a review-type analysis.In this paper,we use bibliometric analysis combined with theoretical analysis and pathfinding network scaling to visualize data and objectively count the development process,current research,and development trend of the corresponding field by analyzing the literature of the specified field.This paper presents the data visualization by analyzing the literature of the specified field to objectively count the development process,current research and development trend of the corresponding field.This paper presents a retrospective analysis and review of the articles on exosomes in the last 20 years(2001–2021)based on the bibliometric analysis method of CiteSpace software,while presenting the problems of current research and discussing the challenges encountered in the future,hoping to provide new ideas for the development of research in this field.展开更多
CovID-19,also known as coronavirus disease 2019,is a novel coronavirus disease with high infectivity,strong heterogeneity,and long incubation period(generally 3-14 days).Its main symptoms and signs include fever,dry c...CovID-19,also known as coronavirus disease 2019,is a novel coronavirus disease with high infectivity,strong heterogeneity,and long incubation period(generally 3-14 days).Its main symptoms and signs include fever,dry cough,nasal congestion,fatigue,disorientation,lymphopenia,and dyspnea.The short-term and long-term impacts of covID-19 on human health,particularly its effects on human reproduction and offspring development,continue to receive significant concerns,as they may lead to potential sequelae for several decades or even centuries.展开更多
基金supported in part by the National Natural Science Foundation of China(No.31971242,12032007)the Chongqing Science and Technology Bureau(China)(No.cstc2021jsyj-yzysbA0057)the Joint Medical Research Project of Chongqing Science and Technology Bureau and Chongqing Health Commission(China)(No.2018ZDXM032).
文摘Atherosclerotic cardiovascular disease and its complications are a high-incidence disease worldwide.Numerous studies have shown that blood flow shear has a huge impact on the function of vascular endothelial cells,and it plays an important role in gene regulation of pro-inflammatory,pro-thrombotic,pro-oxidative stress,and cell permeability.Many impor-tant endothelial cell mechanosensitive genes have been discovered,including KLK10,CCN gene family,NRP2,YAP,TAZ,HIF-1α,NF-kB,FOS,JUN,TFEB,KLF2/KLF4,NRF2,and ID1.Some of them have been intensively studied,whereas the relevant regulatory mechanism of other genes remains unclear.Focusing on these mechanosensitive genes will provide new strategies for therapeutic intervention in atherosclerotic vascular disease.Thus,this article reviews the mechanosensitive genes affecting vascular endothelial cells,including classical pathways and some newly screened genes,and summarizes the latest research progress on their roles in the pathogenesis of atherosclerosis to reveal effective therapeutic targets of drugs and provide new insights foranti-atherosclerosis.
基金This work was supported in part by grants from the National Natural Science Foundation of China(12032007,31971242)Chongqing Research Program of Basic research and Frontier Technology(cstc2019jcyj-zdxmX0028)+1 种基金JinFeng Laboratory Foundation,Chongqing,China(jfkyjf202203001)Shanghai Clinical Research Center for Interventional Medicine(19MC1910300).
文摘Purpose:This review aims to explore the history,research hotspots,and emerging trends of drug-eluting stents(DES)in the last two decades from the perspective of structural and temporal dynamics.Methods:Publications on DES were retrieved from WoSCC.The bibliometric tools including CiteSpace and HistCite were used to identify the historical features,the evolution of active topics,and emerging trends on the DES field.Results:In the last 20 years,the field of DES is still in the hot phase and there is a wide range of extensive scientific collaborations.In addition,active topics emerge in different periods,as evidenced by a total of 41 disciplines,511 keywords,and 1377 papers with citation bursts.Keyword clustering anchored five emerging research subfields,namely#0 dual antiplatelet therapy,#3 drug-coated balloon,#4 bifurcation,5#rotational atherectomy,and 6#quantitative flow ratio.The keyword alluvial map shows that the most persistent research concepts in this field are thrombosis,restenosis,etc.,and the emerging keywords are paclitaxel eluting balloon,coated balloon,drug-eluting balloon,etc.There are 7 recent research subfields anchored by reference clustering,namely#2 dual antiplatelet therapy,#4 drug-coated balloon,#5 peripheral artery disease,#8 fractional flow reserve,#10 bioresorbable vascular scaffold,#13 intravascular ultrasound,#14 biodegradable polymer.Conclusion:The findings based on the bibliometric studies provide the current status and trends in DES research and may help researchers to identify hot topics and explore new research directions in this field.
基金This work was supported by grants from the Natural Science Foundation of Chongqing(cstc2020jcyj-msxmX0330,cstc2021jsyj-yzysbA0057,and cstc2019jcyj-zdxmX0028)the National Natural Science Foundation of China(31971242 and 12032007)+4 种基金the Project of Tutorial System of Medical Undergraduate in Lab Teaching&Management Center in Chongqing Medical University(LTMCMTS202005 and LTMCMTS202110)the JinFeng Laboratory Foundation of Chongqing(jfkyjf202203001)the Scientific and Technological Research Program of Chongqing Municipal Education Commission(KJQN202200426)the Scientific Research,the CQMU Program for Youth Innovation in Future Medicine(W0015)the Innovation Experimental Project of Chongqing Medical University(SRIEP202105).
文摘Multiple signal strategies remarkably improve the accuracy and efficiency of electrochemiluminescence(ECL)immunoassays,but the lack of potential-resolved luminophore pairs and chemical cross talk hinders their development.In this study,we synthesized a series of gold nanoparticles(AuNPs)/reduced graphene oxide(Au/rGO)composites as adjustable oxygen reduction reaction and oxygen evolution reaction catalysts to promote and modulate tris(2,2′-bipyridine)ruthenium(II)(Ru(bpy)_(3)^(2+))’s multisignal luminescence.With the increase in the diameter of AuNPs(3 to 30 nm),their ability to promote Ru(bpy)_(3)^(2+)’s anodic ECL was first impaired and then strengthened,and cathodic ECL was first enhanced and then weakened.Au/rGOs with medium-small and medium-large AuNP diameters remarkably increased Ru(bpy)_(3)^(2+)’s cathodic and anodic luminescence,respectively.Notably,the stimulation effects of Au/rGOs were superior to those of most existing Ru(bpy)_(3)^(2+)co-reactants.Moreover,we proposed a novel ratiometric immunosensor construction strategy using Ru(bpy)_(3)^(2+)’s luminescence promoter rather than luminophores as tags of antibodies to achieve signal resolution.This method avoids signal cross talk between luminophores and their respective co-reactants,which achieved a good linear range of 10−7 to 10−1 ng/ml and a limit of detection of 0.33 fg/ml for detecting carcinoembryonic antigen.This study addresses the previous scarcity of the macromolecular co-reactants of Ru(bpy)_(3)^(2+),broadening its application in biomaterial detection.Furthermore,the systematic clarification of the detailed mechanisms for converting the potential-resolved luminescence of Ru(bpy)_(3)^(2+)could facilitate an in-depth understanding of the ECL process and should inspire new designs of Ru(bpy)_(3)^(2+)luminescence enhancers or applications of Au/rGOs to other luminophores.This work removes some impediments to the development of multisignal ECL biodetection systems and provides vitality into their widespread applications.
基金This work was supported by the National Natural Science Foundation(81972146 to Chengqi He)the Department of Science and Technology of Sichuan Province(20YYJC3320,2020YJ0210 to Chengqi He)+1 种基金West China Hospital of Sichuan University(ZYGD18018 and HX-20l9-nCoV-011to Chengqi He)West China Nursing Discipline Development Special Fund Project,Sichuan University(HXHL20001 to Chengqi He).
文摘Dear Editor,Glucocorticoids(GCs)often cause detrimental side effects,including osteoporosis and osteonecrosis,with no specific drugs available to circumvent these problems.1 Cellular senescence plays an essential role in bone loss,2,3 where senescent cells release various factors,termed senescence-associated secretory phenotype(SASP),which influences neighboring cells and disrupts normal tissues.2 Glucocorticoids can induce senescence in bone cells in young mice;2 however,the effects of GCs on senescence and SASP during bone metabolism in adult mice are unclear.
基金the National Natural Science Foundation of China(12032007,31971242)Chongqing Research Program of Basic research and Frontier Technology(cstc2019jcyjzdxmX0028,cstc2020jscx-msxmX0132)。
文摘Exosomes are membrane vesicles that exist in pathological or normal cells and are considered to be specifically secreted,contain complex RNAs and proteins,participate in intercellular communication functions,and have functions in organismal immune response,antigen presentation,cell migration,cell differentiation,and tumor invasion.In recent years,with the explosion of the development of minimally invasive diagnostics,the research of exosomes has been rapidly developed,and the number of articles published in this field has increased dramatically,and many articles summarize the published papers in a review-type analysis.In this paper,we use bibliometric analysis combined with theoretical analysis and pathfinding network scaling to visualize data and objectively count the development process,current research,and development trend of the corresponding field by analyzing the literature of the specified field.This paper presents the data visualization by analyzing the literature of the specified field to objectively count the development process,current research and development trend of the corresponding field.This paper presents a retrospective analysis and review of the articles on exosomes in the last 20 years(2001–2021)based on the bibliometric analysis method of CiteSpace software,while presenting the problems of current research and discussing the challenges encountered in the future,hoping to provide new ideas for the development of research in this field.
基金supported by the Novel Coronavirus Infection and Prevention Emergency Scientific Research Special Project of Chongqing Municipal Education Commission,China(No.KYYJ202001)G.X.Wang,and the State Key Project Specialized for Infectious Diseases(China)(No.2017ZX10201201-001-005)to Y.M.Wang.
文摘CovID-19,also known as coronavirus disease 2019,is a novel coronavirus disease with high infectivity,strong heterogeneity,and long incubation period(generally 3-14 days).Its main symptoms and signs include fever,dry cough,nasal congestion,fatigue,disorientation,lymphopenia,and dyspnea.The short-term and long-term impacts of covID-19 on human health,particularly its effects on human reproduction and offspring development,continue to receive significant concerns,as they may lead to potential sequelae for several decades or even centuries.