Stem cell therapies have made great progress in the treatment of diabetic wounds during recent decades,while their short in vivo residence,alloimmune reactions,undesired behaviors,and dramatic losses of cell functions...Stem cell therapies have made great progress in the treatment of diabetic wounds during recent decades,while their short in vivo residence,alloimmune reactions,undesired behaviors,and dramatic losses of cell functions still hinder the translation of them into clinic.Here,inspired by the natural components of stem cell niches,we presented novel microfluidic hydrogel microcarriers with extracellular matrix(ECM)-like composition and adipose-derived stem cells(ADSCs)encapsulation for diabetic wound healing.As the hydrogel was synthesized by conjugating hyaluronic acid methacryloyl(HAMA)onto the Fibronectin(FN)molecule chain(FN-HAMA),the laden ADSCs in the microcarriers showed improved bioactivities and pro-regenerative capabilities.Based on these features,we have demonstrated that these ADSCs microcarriers exhibited significant promotion of neovascularization,follicular rejuvenation,and collagen deposition in a mouse diabetic wound model.These results indicated that the stem cell niche-inspired FN-HAMA microcarriers with ADSCs encapsulation have great clinical potential for diabetic wound treatment.展开更多
Skin damage resulting from burns,injuries,or diseases can lead to significant functional and esthetic deficits.However,traditional treatments,such as skin grafting,have limitations including limited donor skin availab...Skin damage resulting from burns,injuries,or diseases can lead to significant functional and esthetic deficits.However,traditional treatments,such as skin grafting,have limitations including limited donor skin availability,poor aesthetics,and functional impairment.Skin tissue engineering provides a promising alternative,with engineered artificial skins offering a highly viable avenue.Engineered artificial skin is designed to mimic or replace the functions of natural human skin and find applications in various medical treatments,particularly for severe burns,chronic wounds,and other skin injuries or defects.These artificial skins aim to promote wound healing,provide temporary coverage,permanent skin replacement,and restore the skin’s barrier function.Artificial skins have diverse applications in medicine and wound care,addressing burns,chronic wounds,and traumatic injuries.They also serve as valuable tools for research in tissue engineering,offering experimental models for studying wound healing mechanisms,testing new biomaterials,and exploring innovative approaches to skin regeneration.This review provides an overview of current construction strategies for engineered artificial skin,including cell sources,biomaterials,and construction techniques.It further explores the primary application areas and future prospects of artificial skin,highlighting their potential to revolutionize skin reconstruction and advance the field of regenerative medicine.展开更多
Chronic wounds are characterized by prolonged healing processes and poor prognoses,which have substantially impacted human health and daily life.Traditional treatment strategies have various limitations and drawbacks....Chronic wounds are characterized by prolonged healing processes and poor prognoses,which have substantially impacted human health and daily life.Traditional treatment strategies have various limitations and drawbacks.Therefore,fully effective therapeutic approaches remain urgently needed.Stem cell(SC)-based therapies have drawn significant attention for their abilities of immunomodulation and pro-regeneration.It has been demon-strated that stem cells(SCs)can improve angiogenesis,collagen deposition,and hair rejuvenation,thus facilitating wound healing.In addition,attempts were performed to facilitate the cell survival,function,retention,and en-graftment of the delivered SCs.In this review,we first introduce the pathological process involved in chronic wound healing.Following that,the mechanism of SCs in promoting chronic wound repair is discussed in detail.Then,we highlight recent SC-based therapies for chronic wound repair developments.Finally,we present our views on the remaining challenges and future trends of SC-based therapies for chronic wound treatment.展开更多
基金supported by the National Key Research and Development Program of China(2020YFA0908200)the National Natural Science Foundation of China(81974288,T2225003 and 52073060)+2 种基金the Strategic Priority Research Program of the Chinese Academy of Science(XDA16021103)the Guangdong Basic and Applied Basic Research Foundation(2021B1515120054)the Shenzhen Fundamental Research Program(JCYJ20190813152616459 and JCYJ20210324133214038).
文摘Stem cell therapies have made great progress in the treatment of diabetic wounds during recent decades,while their short in vivo residence,alloimmune reactions,undesired behaviors,and dramatic losses of cell functions still hinder the translation of them into clinic.Here,inspired by the natural components of stem cell niches,we presented novel microfluidic hydrogel microcarriers with extracellular matrix(ECM)-like composition and adipose-derived stem cells(ADSCs)encapsulation for diabetic wound healing.As the hydrogel was synthesized by conjugating hyaluronic acid methacryloyl(HAMA)onto the Fibronectin(FN)molecule chain(FN-HAMA),the laden ADSCs in the microcarriers showed improved bioactivities and pro-regenerative capabilities.Based on these features,we have demonstrated that these ADSCs microcarriers exhibited significant promotion of neovascularization,follicular rejuvenation,and collagen deposition in a mouse diabetic wound model.These results indicated that the stem cell niche-inspired FN-HAMA microcarriers with ADSCs encapsulation have great clinical potential for diabetic wound treatment.
基金supported by grants from National Natural Science Foundation of China(NO.81974288)National Natural Science Foundation of China(NO.82302812)Natural Science Foundation of Zhejiang Province of China(LQ22E030004),。
文摘Skin damage resulting from burns,injuries,or diseases can lead to significant functional and esthetic deficits.However,traditional treatments,such as skin grafting,have limitations including limited donor skin availability,poor aesthetics,and functional impairment.Skin tissue engineering provides a promising alternative,with engineered artificial skins offering a highly viable avenue.Engineered artificial skin is designed to mimic or replace the functions of natural human skin and find applications in various medical treatments,particularly for severe burns,chronic wounds,and other skin injuries or defects.These artificial skins aim to promote wound healing,provide temporary coverage,permanent skin replacement,and restore the skin’s barrier function.Artificial skins have diverse applications in medicine and wound care,addressing burns,chronic wounds,and traumatic injuries.They also serve as valuable tools for research in tissue engineering,offering experimental models for studying wound healing mechanisms,testing new biomaterials,and exploring innovative approaches to skin regeneration.This review provides an overview of current construction strategies for engineered artificial skin,including cell sources,biomaterials,and construction techniques.It further explores the primary application areas and future prospects of artificial skin,highlighting their potential to revolutionize skin reconstruction and advance the field of regenerative medicine.
基金supported by grants from the National Natural Science Foundation of China (No.81974288)the National Natural Science Foundation of China (No.82101184)+1 种基金Shenzhen Fundamental Research Program (No.JCYJ20210324102809024)Shenzhen PhD Start-up Program (No.RCBS20210609103713045).
文摘Chronic wounds are characterized by prolonged healing processes and poor prognoses,which have substantially impacted human health and daily life.Traditional treatment strategies have various limitations and drawbacks.Therefore,fully effective therapeutic approaches remain urgently needed.Stem cell(SC)-based therapies have drawn significant attention for their abilities of immunomodulation and pro-regeneration.It has been demon-strated that stem cells(SCs)can improve angiogenesis,collagen deposition,and hair rejuvenation,thus facilitating wound healing.In addition,attempts were performed to facilitate the cell survival,function,retention,and en-graftment of the delivered SCs.In this review,we first introduce the pathological process involved in chronic wound healing.Following that,the mechanism of SCs in promoting chronic wound repair is discussed in detail.Then,we highlight recent SC-based therapies for chronic wound repair developments.Finally,we present our views on the remaining challenges and future trends of SC-based therapies for chronic wound treatment.
