Silane coupling regent （3-mercaptopropyl trimethoxysilane （MPTS）） was prepared on the single-crystal silicon substrate to form 2-dimensional self-assembled monolayers （SAMs）. The growth behavior of SAMs formed f...Silane coupling regent （3-mercaptopropyl trimethoxysilane （MPTS）） was prepared on the single-crystal silicon substrate to form 2-dimensional self-assembled monolayers （SAMs）. The growth behavior of SAMs formed from 3-MPTS was investigated using atomic force microscopy （AFM）, contact angle measurements, ellipsometry, and X-ray photoelectron spectroscopy （XPS）. The formation behavior of MPTS SAMs was investigated by a series of AFM images and the roughness of MPTS SAMs on silicon substrates with the assembling time from 1 min to 24 h. The water contact angle measurements indicated the growth behavior of MPTS that correlated with the AFM measurements at different immersion times, too. The chemical states of the typical elements in the MPTS SAMs were analyzed using X-ray photoelectron spectroscopy. The results show that MPTS is self-assembled on the substrate.展开更多
Formaldehyde(FA)plays critical roles in Alzheimer's disease and the associations between FA and Alzheimer's disease(AD)are still obscure.To reveal FA fluxes in the Alzheimer's disease brain,an activity-bas...Formaldehyde(FA)plays critical roles in Alzheimer's disease and the associations between FA and Alzheimer's disease(AD)are still obscure.To reveal FA fluxes in the Alzheimer's disease brain,an activity-based fluorescence probe NP-FA with superb blood-brain barrier permeable abilities was exquisitely designed.The probe responded to FA with significant fluorescence increases(F/F0=81),thus laying the foundation for the sensitive detection of FA in cuvette and in vivo.Moreover,the probe also possessed some fasci-nating performances,including endoplasmic reticulum(ER)-targeting abilities,good one-photon/two-photon absorption properties,and appropriate hydrophobicity property(log P=2.34±0.05).As a result,the probe can readily reflect the overproduction of FA con-tent in live cells under ER stress by high-fidelity two-photon imaging.More interestingly,ex vivo imaging of AD brains and two-photon imaging of AD slice tissues visually disclosed that the FA level of AD brain is much higher than that of the normal brain.This work afforded a specific activity-based probe for the imaging of FA in the AD mouse brains,which could be further extended to FA-related studies in Alzheimer's disease.展开更多
基金This work was financially supported by the National Natural Science Foundation of China (No.50475023)the Nano Foundation of Shanghai Technology Committee (No.0252nm014)the State Key Laboratory Fund (No.0102) in State Key Laboratory for Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Science
文摘Silane coupling regent （3-mercaptopropyl trimethoxysilane （MPTS）） was prepared on the single-crystal silicon substrate to form 2-dimensional self-assembled monolayers （SAMs）. The growth behavior of SAMs formed from 3-MPTS was investigated using atomic force microscopy （AFM）, contact angle measurements, ellipsometry, and X-ray photoelectron spectroscopy （XPS）. The formation behavior of MPTS SAMs was investigated by a series of AFM images and the roughness of MPTS SAMs on silicon substrates with the assembling time from 1 min to 24 h. The water contact angle measurements indicated the growth behavior of MPTS that correlated with the AFM measurements at different immersion times, too. The chemical states of the typical elements in the MPTS SAMs were analyzed using X-ray photoelectron spectroscopy. The results show that MPTS is self-assembled on the substrate.
基金the National Natural Science Foundation of China(Nos.22174034,21804033,and 21625503)the Natural Science Foundation of Hubei Province(2021CFB305)。
文摘Formaldehyde(FA)plays critical roles in Alzheimer's disease and the associations between FA and Alzheimer's disease(AD)are still obscure.To reveal FA fluxes in the Alzheimer's disease brain,an activity-based fluorescence probe NP-FA with superb blood-brain barrier permeable abilities was exquisitely designed.The probe responded to FA with significant fluorescence increases(F/F0=81),thus laying the foundation for the sensitive detection of FA in cuvette and in vivo.Moreover,the probe also possessed some fasci-nating performances,including endoplasmic reticulum(ER)-targeting abilities,good one-photon/two-photon absorption properties,and appropriate hydrophobicity property(log P=2.34±0.05).As a result,the probe can readily reflect the overproduction of FA con-tent in live cells under ER stress by high-fidelity two-photon imaging.More interestingly,ex vivo imaging of AD brains and two-photon imaging of AD slice tissues visually disclosed that the FA level of AD brain is much higher than that of the normal brain.This work afforded a specific activity-based probe for the imaging of FA in the AD mouse brains,which could be further extended to FA-related studies in Alzheimer's disease.
