Lanthanide-containing persistent luminescence materials are promising candidates for a wide range of applications by virtue of splendid superiorities in afterglow performance.However,it is a crucial challenge to achie...Lanthanide-containing persistent luminescence materials are promising candidates for a wide range of applications by virtue of splendid superiorities in afterglow performance.However,it is a crucial challenge to achieve high-quality afterglow materials at the attractive nanoscale,with uniform size,controllable morphology,and satisfying brightness.Herein,a bottom-up approach was developed to construct the high-quality afterglow nanoparticles,incorporating luminescent lanthanide complex and organic molecular ingredients under mild conditions.These nanoparticles exhibited intrinsic lanthanide luminescence with superbright red afterglow(>10 cd m^(−2))in a homogeneous solution.The afterglow solution with excellent processability can serve as ideal building blocks for the on-demand fabrication of functional nanomaterials.Water-dispersible afterglow nanoparticles with state-of-the-art high brightness were uniformly constructed to perform whole-blood lateral flow assay of procalcitonin with a naked-eye detection limit of 0.5 ng mL^(−1),promoting the point-of-care testing development.展开更多
ABSTRACT The efficient detection of tumor markers is crucial to early cancer diagnosis and monitoring.Here,we propose a unique“two birds with one stone”dissolution-enhanced afterglow bioassay based on amino-function...ABSTRACT The efficient detection of tumor markers is crucial to early cancer diagnosis and monitoring.Here,we propose a unique“two birds with one stone”dissolution-enhanced afterglow bioassay based on amino-functionalized mesoporous SiO2 nanoparticles(MSNs-NH2)encapsulated Eu(OH)CO_(3)nanoprobes.These nanoprobes(i)stably conjugate to the antibody and(ii)are highly soluble in the acidic enhancer solution,and provide a high Eu3+concentration.The released Eu3+ions further activate the photochemical afterglow system to determine the alpha-fetoprotein(AFP)levels in clinical serum samples.Without the use of a real-time excitation source,the interference of autofluorescence and scattering from blood can be avoided.The demonstrated limit of detection(LOD)for AFP was as low as 0.12 ng·mL^(−1).This covalently connected dissolution-enhanced luminescent bioassay(DELBA)and novel afterglow system shows tremendous potential for ultrasensitive and rapid clinical diagnosis.展开更多
基金supported by the National Key R&D Program of China(2017YFA0205100)the National Natural Science Foundation of China(21937003)。
文摘Lanthanide-containing persistent luminescence materials are promising candidates for a wide range of applications by virtue of splendid superiorities in afterglow performance.However,it is a crucial challenge to achieve high-quality afterglow materials at the attractive nanoscale,with uniform size,controllable morphology,and satisfying brightness.Herein,a bottom-up approach was developed to construct the high-quality afterglow nanoparticles,incorporating luminescent lanthanide complex and organic molecular ingredients under mild conditions.These nanoparticles exhibited intrinsic lanthanide luminescence with superbright red afterglow(>10 cd m^(−2))in a homogeneous solution.The afterglow solution with excellent processability can serve as ideal building blocks for the on-demand fabrication of functional nanomaterials.Water-dispersible afterglow nanoparticles with state-of-the-art high brightness were uniformly constructed to perform whole-blood lateral flow assay of procalcitonin with a naked-eye detection limit of 0.5 ng mL^(−1),promoting the point-of-care testing development.
基金National Key R&D Program of China(No.2017YFA0205100)the National Natural Science Foundation of China(No.22101052)for financial support.
文摘ABSTRACT The efficient detection of tumor markers is crucial to early cancer diagnosis and monitoring.Here,we propose a unique“two birds with one stone”dissolution-enhanced afterglow bioassay based on amino-functionalized mesoporous SiO2 nanoparticles(MSNs-NH2)encapsulated Eu(OH)CO_(3)nanoprobes.These nanoprobes(i)stably conjugate to the antibody and(ii)are highly soluble in the acidic enhancer solution,and provide a high Eu3+concentration.The released Eu3+ions further activate the photochemical afterglow system to determine the alpha-fetoprotein(AFP)levels in clinical serum samples.Without the use of a real-time excitation source,the interference of autofluorescence and scattering from blood can be avoided.The demonstrated limit of detection(LOD)for AFP was as low as 0.12 ng·mL^(−1).This covalently connected dissolution-enhanced luminescent bioassay(DELBA)and novel afterglow system shows tremendous potential for ultrasensitive and rapid clinical diagnosis.
