The aim of this work was to inhibit biofilm formation by taking advantages of bacterial surface display technology in combination with cell membrane chromatography.A recombinant protein INPAidH was constructed by fusi...The aim of this work was to inhibit biofilm formation by taking advantages of bacterial surface display technology in combination with cell membrane chromatography.A recombinant protein INPAidH was constructed by fusing a quorum signal hydrolase AidH to the C-terminus of the ice nucleation protein(INP).Expression of INP-AidH was achieved on E.coli cell surface at an expression level of 30%of total membrane proteins.Activity of INP-AidH on cell membranes was confirmed in degrading the quorum signal C6-HSL as well as inhibiting bacterial biofilm.Immobilization of INP-AidH anchored cell membranes on silica gel particles was facilitated by taking advantages of cell membrane chromatography.The functionalized silica gel particles also exhibit activities in degrading C6-HSL and inhibiting bacterial biofilm.This article presents a new approach to prevent biofilm formation of silica-based materials.展开更多
At the invitation of the 48 Group Club of Britain,the Institute of International&European Affairs(IIEA)of Ireland and the Ministry of Foreign Affairs of Iceland,a six-member delegation led by Mr.Ai Ping,Member of ...At the invitation of the 48 Group Club of Britain,the Institute of International&European Affairs(IIEA)of Ireland and the Ministry of Foreign Affairs of Iceland,a six-member delegation led by Mr.Ai Ping,Member of the National Committee of CPPCC and CAFIU VicePresident visited Britain,Ireland展开更多
From December 11 to 20,2013,I joined the CAFIU delegation to visit Britain,Poland and France as CAFIU Council Member.Rich in content,the visit not only promoted the exchanges and friendship between CAFIU and tradition...From December 11 to 20,2013,I joined the CAFIU delegation to visit Britain,Poland and France as CAFIU Council Member.Rich in content,the visit not only promoted the exchanges and friendship between CAFIU and traditional partners in the three countries,but also deepened my understanding of the three countries and relevant issues of common concern.展开更多
At the invitation of the 48 Group Club of Britain,the Civic Association"Polish House"of Poland and the"Asia Horizon 2020"of France,a fivemember delegation of the Chinese Association for Internation...At the invitation of the 48 Group Club of Britain,the Civic Association"Polish House"of Poland and the"Asia Horizon 2020"of France,a fivemember delegation of the Chinese Association for International Understanding(hereinafter referred to as"CAFIU")led by Zheng Yao,Deputy Secretary-general of CAFIU visited the three countries from December 11 to 20,2013.展开更多
This year marks the 130th anniversary of the founding of the first Chinese legation in Berlin and the 35th anniversary of the establishment of diplomatic relations between Germany and China. This year also marks the 2...This year marks the 130th anniversary of the founding of the first Chinese legation in Berlin and the 35th anniversary of the establishment of diplomatic relations between Germany and China. This year also marks the 20th anniversary of cooperation between Friedrich-Ebert Foundation(FES) and CAFIU.展开更多
文摘对计及经济、环境因素的电力系统发电调度问题(Economic Environmental Dispatching,EED)进行研究,提出一种采用改进多目标灰狼算法的发电调度规划方案。构建基于发电燃料成本、污染气体排放量和节点电压偏移量等指标的多目标EED模型,并采用改进的多目标灰狼算法进行求解,以得到更优的Pareto前沿和折中解。设计多度量自适应FCM算法对灰狼算法(Gray Wolf Algorithm,GWA)种群多样性进行分析,重新定义狼群层级结构和Pareto前沿规模控制策略,并在此基础上提出反向学习和变异进化策略,以提升GWA全局收敛性能。仿真结果表明,改进的GWA具有优秀全局寻优能力,而且基于改进多目标灰狼算法得到的Pareto前沿和折中解更具可行性和优越性。
基金Funded by the National Natural Science Foundation of China(No.31771032)。
文摘The aim of this work was to inhibit biofilm formation by taking advantages of bacterial surface display technology in combination with cell membrane chromatography.A recombinant protein INPAidH was constructed by fusing a quorum signal hydrolase AidH to the C-terminus of the ice nucleation protein(INP).Expression of INP-AidH was achieved on E.coli cell surface at an expression level of 30%of total membrane proteins.Activity of INP-AidH on cell membranes was confirmed in degrading the quorum signal C6-HSL as well as inhibiting bacterial biofilm.Immobilization of INP-AidH anchored cell membranes on silica gel particles was facilitated by taking advantages of cell membrane chromatography.The functionalized silica gel particles also exhibit activities in degrading C6-HSL and inhibiting bacterial biofilm.This article presents a new approach to prevent biofilm formation of silica-based materials.
文摘At the invitation of the 48 Group Club of Britain,the Institute of International&European Affairs(IIEA)of Ireland and the Ministry of Foreign Affairs of Iceland,a six-member delegation led by Mr.Ai Ping,Member of the National Committee of CPPCC and CAFIU VicePresident visited Britain,Ireland
文摘From December 11 to 20,2013,I joined the CAFIU delegation to visit Britain,Poland and France as CAFIU Council Member.Rich in content,the visit not only promoted the exchanges and friendship between CAFIU and traditional partners in the three countries,but also deepened my understanding of the three countries and relevant issues of common concern.
文摘At the invitation of the 48 Group Club of Britain,the Civic Association"Polish House"of Poland and the"Asia Horizon 2020"of France,a fivemember delegation of the Chinese Association for International Understanding(hereinafter referred to as"CAFIU")led by Zheng Yao,Deputy Secretary-general of CAFIU visited the three countries from December 11 to 20,2013.
文摘This year marks the 130th anniversary of the founding of the first Chinese legation in Berlin and the 35th anniversary of the establishment of diplomatic relations between Germany and China. This year also marks the 20th anniversary of cooperation between Friedrich-Ebert Foundation(FES) and CAFIU.
