Objective:In this study,we aim to enhance the anti-prostate cancer efficacy of cabazitaxel(CTX)and reduce its immunosuppression and systemic toxicity by developing CTX-loaded liposomes modified with ginsenoside Rk1(Rk...Objective:In this study,we aim to enhance the anti-prostate cancer efficacy of cabazitaxel(CTX)and reduce its immunosuppression and systemic toxicity by developing CTX-loaded liposomes modified with ginsenoside Rk1(Rk1/CTX-Lip).Methods:Physical and chemical properties of Rk1/CTX-Lip were investigated.We evaluated the biological functions of Rk1/CTXLip,both in vitro and in vivo.A subcutaneous prostate cancer(RM-1)-bearing mouse model was established to study the efficacy of Rk1/CTX-Lip inhibition in tumors.Simultaneously,a Candida albicans infection model was established in tumor-bearing mice to study the infection-relieving efficacy of Rk1/CTX-Lip.Finally,biocompatibility and in vivo safety of Rk1/CTX-Lip were evaluated.Results:We successfully prepared Rk1/CTX-Lip,achieving high CTX encapsulation efficiency(97.24±0.75)%and physical stability.Rk1/CTX-Lip demonstrated evasion of macrophage phagocytosis,effective tumor tissue targeting,and a significant reduction(>50%)in average tumor volume compared with Chol/CTX-Lip.Moreover,it relieved the concurrent infection burden and effectively regulated immune organs and cells,demonstrating superior biocompatibility.Conclusion:Rk1/CTX-Lip presents a promising new therapy for prostate cancer and holds potential for relieving concurrent fungal infections in cancer patients with low immunity.展开更多
Congestion causes many externalities for the society including time delays, excessive fuel consumption, air pollution noise and safety concerns. In Shanghai, various policy options have been explored, piloted or appli...Congestion causes many externalities for the society including time delays, excessive fuel consumption, air pollution noise and safety concerns. In Shanghai, various policy options have been explored, piloted or applied; however, not all of them may be understood and accepted by the public. A survey was conducted to investigate people’s attitudes towards several policy options. The main findings reveal that Shanghai residents are resis tant to certain policies, such as congestion charges, higher parking charges in congested areas and car restrictions. Instead, they favo public transport provisions. The paper suggests that there is a case for promoting public transport and more efficient trips when the car ownership is still low, and for investing in a policy of educat ing the public on the ‘true’ costs and causes of congestion before embarking on an intensive policy of congestion charges or restric tions.展开更多
[Objective] This study aimed to examine indicative roles of texture representing soil organic carbon presence and variability subsequent to cultivation under cold temperate climates with seasonal freeze-thaw events. [...[Objective] This study aimed to examine indicative roles of texture representing soil organic carbon presence and variability subsequent to cultivation under cold temperate climates with seasonal freeze-thaw events. [Method] Three chronosequences were selected for paired comparisons. Soil samples were collected at six depths with a 10 cm increment. Analysis of variance with general linear model and regression was performed for statistical analysis. [Result] In seasonally frozen soils where fragmentation of macroaggregates was stimulated, soil organic carbon level was positively associated with clay + silt proportion due to a wider textural range, better than sole clay content. Exponential function better fitted the experimental data to present progressively increased effectiveness of clay + silt content in maintaining carbon. Clay content explained 12%-41% and 14%-43% of variation via linear and exponential functions, respectively. Accordingly, clay + silt content explained 47%-65% and 46%-70%. [Conclusion] Texture reflected soil organic carbon occurrence as consequences of reclamation. For seasonally frozen soils with wider textural ranges, it is robust to adapt clay + silt content as dependent variable and exponential function. The generated algorithms provided an available pathway to estimate soil organic carbon losses following cultivation and to evaluate soil fertility.展开更多
[Objective] This study aimed to characterize constituents of phosphorus(P)fractionation as well as reciprocities among factors in sediments of a freshwater shallow lake. [Method] Surface sediment was discretely sample...[Objective] This study aimed to characterize constituents of phosphorus(P)fractionation as well as reciprocities among factors in sediments of a freshwater shallow lake. [Method] Surface sediment was discretely sampled at 24 sites through Van Veen grabs. Based on a modified sequential extraction scheme, P fractionation was determined as Fe/Al bound P(Fe/Al-P), Ca bound P(Ca-P), solute and reductive P(S/R-P) and organic P(OP). Curve estimation and Pearson product-moment correlation were employed for statistical analysis. [Result] Total P(TP) content ranged from 443 to 774 mg/kg. Inorganic P(IP) was the major component of TP,of which Ca-P was dominated with an average of 51%±9.7%. Average contents of P fractionation were in the following order: Ca-P(51%) > OP(29%) > S/R-P(8%) >Fe/Al-P(7%). The molar ratio of Fe to P was 11- 20, close to the threshold value of P leaching. [Conclusion] In freshwater shallow lakes, IP and Ca-P were prone to be relatively high, whereas Fe/Al-P was low compared with deep lakes. Occurrence of spatially monotonic gradient indicated the primary causation of anthropogenic sources. Imminent sediment P liberation was also expected. Close associations among TP, Fe/Al-P and Ca-P, implying that anthropogenic P was mainly bound to metals in particulates. Significant correlations of TOC and P fractionation highlighted endogenous mechanism and authigenic origin in sediments.展开更多
Variations of phosphorus （P） and its species in surface sediment of Baiyangdian Lake, a eutrophic shallow lake located in North China, were investigated through combination of field survey and numerical calculation ...Variations of phosphorus （P） and its species in surface sediment of Baiyangdian Lake, a eutrophic shallow lake located in North China, were investigated through combination of field survey and numerical calculation based on cluster analysis. P fractionation was performed by a sequential extraction scheme, categorized as loosely bound P （NH4Cl-P）, reductant soluble P （BD-P）, metallic oxide bound P （NaOH-P）, calcium bound P （HCl-P） and organic P （Org-P）. P concentrations exhibited regional similarities and a total of four sub-areas were identified in which the same rank was HCl-P 〉 Org-P 〉 BD-P ,=NaOH-P 〉NH4Cl-E NH4Cl-P, BD-P and Org-P were found to contribute to P enrichment in overlying water column. Specifically, labile Org-P acted as a potential pool with a greater contribution in aerobic layer compared to anaerobic layer. A hysteresis （lag = 4 months） existed when labile Org-P concentration was negatively correlated with aerobic layer thickness. In view of magnitude of identified P contributors in sub-areas, higher potential of P release was present in Fuhe River and Tang River estuary areas. On the basis of calibration and verification, the mathematical model with parameter settings applied in this study was improved to serve as a tool for limnology management and eutrophic control.展开更多
基金supported by the National Natural Science Foundation of China(82373808)Chongqing Natural Science Foundation(cstc2021jcyj-bshX0125)+1 种基金Fundamental Research Funds for the Central Universities(SWURC2020001)the project for Chongqing University Innovation Research Group,Chongqing Education Committee(CXQT20006).
文摘Objective:In this study,we aim to enhance the anti-prostate cancer efficacy of cabazitaxel(CTX)and reduce its immunosuppression and systemic toxicity by developing CTX-loaded liposomes modified with ginsenoside Rk1(Rk1/CTX-Lip).Methods:Physical and chemical properties of Rk1/CTX-Lip were investigated.We evaluated the biological functions of Rk1/CTXLip,both in vitro and in vivo.A subcutaneous prostate cancer(RM-1)-bearing mouse model was established to study the efficacy of Rk1/CTX-Lip inhibition in tumors.Simultaneously,a Candida albicans infection model was established in tumor-bearing mice to study the infection-relieving efficacy of Rk1/CTX-Lip.Finally,biocompatibility and in vivo safety of Rk1/CTX-Lip were evaluated.Results:We successfully prepared Rk1/CTX-Lip,achieving high CTX encapsulation efficiency(97.24±0.75)%and physical stability.Rk1/CTX-Lip demonstrated evasion of macrophage phagocytosis,effective tumor tissue targeting,and a significant reduction(>50%)in average tumor volume compared with Chol/CTX-Lip.Moreover,it relieved the concurrent infection burden and effectively regulated immune organs and cells,demonstrating superior biocompatibility.Conclusion:Rk1/CTX-Lip presents a promising new therapy for prostate cancer and holds potential for relieving concurrent fungal infections in cancer patients with low immunity.
文摘Congestion causes many externalities for the society including time delays, excessive fuel consumption, air pollution noise and safety concerns. In Shanghai, various policy options have been explored, piloted or applied; however, not all of them may be understood and accepted by the public. A survey was conducted to investigate people’s attitudes towards several policy options. The main findings reveal that Shanghai residents are resis tant to certain policies, such as congestion charges, higher parking charges in congested areas and car restrictions. Instead, they favo public transport provisions. The paper suggests that there is a case for promoting public transport and more efficient trips when the car ownership is still low, and for investing in a policy of educat ing the public on the ‘true’ costs and causes of congestion before embarking on an intensive policy of congestion charges or restric tions.
基金Supported by the National Natural Science Foundation of China(41171384,41271414and 41301529)
文摘[Objective] This study aimed to examine indicative roles of texture representing soil organic carbon presence and variability subsequent to cultivation under cold temperate climates with seasonal freeze-thaw events. [Method] Three chronosequences were selected for paired comparisons. Soil samples were collected at six depths with a 10 cm increment. Analysis of variance with general linear model and regression was performed for statistical analysis. [Result] In seasonally frozen soils where fragmentation of macroaggregates was stimulated, soil organic carbon level was positively associated with clay + silt proportion due to a wider textural range, better than sole clay content. Exponential function better fitted the experimental data to present progressively increased effectiveness of clay + silt content in maintaining carbon. Clay content explained 12%-41% and 14%-43% of variation via linear and exponential functions, respectively. Accordingly, clay + silt content explained 47%-65% and 46%-70%. [Conclusion] Texture reflected soil organic carbon occurrence as consequences of reclamation. For seasonally frozen soils with wider textural ranges, it is robust to adapt clay + silt content as dependent variable and exponential function. The generated algorithms provided an available pathway to estimate soil organic carbon losses following cultivation and to evaluate soil fertility.
基金Supported by the National Natural Science Foundation of China(grant No.41171384,41271414 and 41301529)
文摘[Objective] This study aimed to characterize constituents of phosphorus(P)fractionation as well as reciprocities among factors in sediments of a freshwater shallow lake. [Method] Surface sediment was discretely sampled at 24 sites through Van Veen grabs. Based on a modified sequential extraction scheme, P fractionation was determined as Fe/Al bound P(Fe/Al-P), Ca bound P(Ca-P), solute and reductive P(S/R-P) and organic P(OP). Curve estimation and Pearson product-moment correlation were employed for statistical analysis. [Result] Total P(TP) content ranged from 443 to 774 mg/kg. Inorganic P(IP) was the major component of TP,of which Ca-P was dominated with an average of 51%±9.7%. Average contents of P fractionation were in the following order: Ca-P(51%) > OP(29%) > S/R-P(8%) >Fe/Al-P(7%). The molar ratio of Fe to P was 11- 20, close to the threshold value of P leaching. [Conclusion] In freshwater shallow lakes, IP and Ca-P were prone to be relatively high, whereas Fe/Al-P was low compared with deep lakes. Occurrence of spatially monotonic gradient indicated the primary causation of anthropogenic sources. Imminent sediment P liberation was also expected. Close associations among TP, Fe/Al-P and Ca-P, implying that anthropogenic P was mainly bound to metals in particulates. Significant correlations of TOC and P fractionation highlighted endogenous mechanism and authigenic origin in sediments.
基金Acknowledgements This paper was funded by the National Natural Science Foundation of China （Grant Nos. 41171384, 41271414 and 41301529） and Grand Special Science and Technology Project on National Water Pollution Management of China （No. 2008ZX07209-009）.
文摘Variations of phosphorus （P） and its species in surface sediment of Baiyangdian Lake, a eutrophic shallow lake located in North China, were investigated through combination of field survey and numerical calculation based on cluster analysis. P fractionation was performed by a sequential extraction scheme, categorized as loosely bound P （NH4Cl-P）, reductant soluble P （BD-P）, metallic oxide bound P （NaOH-P）, calcium bound P （HCl-P） and organic P （Org-P）. P concentrations exhibited regional similarities and a total of four sub-areas were identified in which the same rank was HCl-P 〉 Org-P 〉 BD-P ,=NaOH-P 〉NH4Cl-E NH4Cl-P, BD-P and Org-P were found to contribute to P enrichment in overlying water column. Specifically, labile Org-P acted as a potential pool with a greater contribution in aerobic layer compared to anaerobic layer. A hysteresis （lag = 4 months） existed when labile Org-P concentration was negatively correlated with aerobic layer thickness. In view of magnitude of identified P contributors in sub-areas, higher potential of P release was present in Fuhe River and Tang River estuary areas. On the basis of calibration and verification, the mathematical model with parameter settings applied in this study was improved to serve as a tool for limnology management and eutrophic control.
