Oil pollution in marine environment is becoming increasingly serious. Oil bioremediation by immobilization technology has been widely studied. However, the effect of bacteria immobilization was limited because of lack...Oil pollution in marine environment is becoming increasingly serious. Oil bioremediation by immobilization technology has been widely studied. However, the effect of bacteria immobilization was limited because of lack of nutrients. In this paper, the organic materials(corn straw, corn cob and corn leaf) were used as carriers. The diesel removal rate achieved by the immobilized bacteria was studied, and the effects of nutrients(e.g. nitrogen and phosphorus) released from organic carriers to the immobilized bacteria were analyzed. Test results indicated that a certain amount of nutrients was released from organic carriers. Additionally, the diesel removal rates achieved by different immobilized bacteria were all higher than those achieved by free bacteria. And, the diesel removal rates achieved by bacteria decreased in the following order: bacteria immobilized on corn straw(79%), bacteria immobilized on corn leaf(70%) and bacteria immobilized on corn cob(43%). These findings indicated two aspects, viz.: the carriers porous structure and the nutrients, which were favorable to biodegradation. Finally, the changes in nitrogen and phosphorus contents that were released from different carriers during biodegradation were studied. The results showed that the rate of diesel biodegradation was fast in the initial phase because of the sufficient nutrients released from carriers. Meanwhile, in the final phase, the rate of diesel biodegradation was relatively slow because of few nutrients released from carriers.展开更多
The biomass of petroleum-degrading bacteria, such as Halomonas spp., is crucial to the alleviation of severe oil spills through bioremediation. In this paper, the bacterium(HDMP1) was isolated and identified. Growth f...The biomass of petroleum-degrading bacteria, such as Halomonas spp., is crucial to the alleviation of severe oil spills through bioremediation. In this paper, the bacterium(HDMP1) was isolated and identified. Growth factors were analysed and optimised through the single-factor experiments, the factor analysis(FA), the principal component analysis(PCA), and the response surface methodology(RSM). Results indicated that HDMP1 was identified as genus Halomonas. In the single-factor experiments, the range of suitable growth conditions for HDMP1 covered: a salt concentration of 2%-4%, a medium pH value of approximately 9, an inoculum concentration of 1.0%, a substrate concentration of 1.0%-1.4%, and a rotation rate of 140 r/min. The evaluation by FA and PCA indicated that three significant growth factors were the salt concentration, the pH value, and the rotation rate. A maximum biomass of HDMP1 was obtained under the conditions covering a salt concentration of 3.5%, a medium pH of 8, and a rotation rate of 151 r/min by optimization.展开更多
The effects of plant hormones for biodegradation of polycyclic aromatic sulfur heterocycles(PASHs)and diesel fuel were studied.Indole butyric acid(IBA)and gibberellin were found to promote biodegradation of DBT and di...The effects of plant hormones for biodegradation of polycyclic aromatic sulfur heterocycles(PASHs)and diesel fuel were studied.Indole butyric acid(IBA)and gibberellin were found to promote biodegradation of DBT and diesel,respectively.Concentrations of plant hormones,pH,temperature,soil moisture and substrate concentrations were optimized in microbial metabolic processes.Two main factors including temperature and IBA concentration were determined by factor analysis in DBT biodegradation.And soil moisture and diesel concentration were important factors in diesel biodegradation.Binding sites between cell surface and DBT or diesel components were performed by molecular operating environment(MOE).This study suggested that plant hormones could be applied to effectively remove pollutants in environment.展开更多
基金financially supported by the National Natural Science Foundation of China (Grant No. 51408347, 51474140)the Scientific Research Fund Project of Introduced Talents (2014RCJJ015)+1 种基金Young Teachers Taleuts Training Plan (BJRC20170502) of Shandong University of Science and Technologythe Science and Technology Projects of Qingdao (Grant No. 15-9-1-58-jch and 15-9-1-32-jch)
文摘Oil pollution in marine environment is becoming increasingly serious. Oil bioremediation by immobilization technology has been widely studied. However, the effect of bacteria immobilization was limited because of lack of nutrients. In this paper, the organic materials(corn straw, corn cob and corn leaf) were used as carriers. The diesel removal rate achieved by the immobilized bacteria was studied, and the effects of nutrients(e.g. nitrogen and phosphorus) released from organic carriers to the immobilized bacteria were analyzed. Test results indicated that a certain amount of nutrients was released from organic carriers. Additionally, the diesel removal rates achieved by different immobilized bacteria were all higher than those achieved by free bacteria. And, the diesel removal rates achieved by bacteria decreased in the following order: bacteria immobilized on corn straw(79%), bacteria immobilized on corn leaf(70%) and bacteria immobilized on corn cob(43%). These findings indicated two aspects, viz.: the carriers porous structure and the nutrients, which were favorable to biodegradation. Finally, the changes in nitrogen and phosphorus contents that were released from different carriers during biodegradation were studied. The results showed that the rate of diesel biodegradation was fast in the initial phase because of the sufficient nutrients released from carriers. Meanwhile, in the final phase, the rate of diesel biodegradation was relatively slow because of few nutrients released from carriers.
基金funded by the National Natural Science Foundation of China(Grant No.51408347)the Open Research Fund Program of Shandong Key Laboratory of Eco-Environmental Science for Yellow River Delta(Binzhou University)(2019KFJJ02)+1 种基金the Major Science and Technology Innovation Projects in Shandong Province(2019JZZY020808)the SDUST Graduate Technology Innovation Project(SDKDYC190321)
文摘The biomass of petroleum-degrading bacteria, such as Halomonas spp., is crucial to the alleviation of severe oil spills through bioremediation. In this paper, the bacterium(HDMP1) was isolated and identified. Growth factors were analysed and optimised through the single-factor experiments, the factor analysis(FA), the principal component analysis(PCA), and the response surface methodology(RSM). Results indicated that HDMP1 was identified as genus Halomonas. In the single-factor experiments, the range of suitable growth conditions for HDMP1 covered: a salt concentration of 2%-4%, a medium pH value of approximately 9, an inoculum concentration of 1.0%, a substrate concentration of 1.0%-1.4%, and a rotation rate of 140 r/min. The evaluation by FA and PCA indicated that three significant growth factors were the salt concentration, the pH value, and the rotation rate. A maximum biomass of HDMP1 was obtained under the conditions covering a salt concentration of 3.5%, a medium pH of 8, and a rotation rate of 151 r/min by optimization.
基金The authors gratefully acknowledge the financial support provided by the Natural Science Foundation of Shandong Province(Grant No.ZR2019BD035)the Open Project Program of State Key Laboratory of Petroleum Pollution Control(Grant No.PPC2017020)the CNPC Research Institute of Safety and Environmental Technology,and the Scientific Research Foundation of Shandong University of Science and Technology for Recruited Talents(Grant No.2016RCJJ016).
文摘The effects of plant hormones for biodegradation of polycyclic aromatic sulfur heterocycles(PASHs)and diesel fuel were studied.Indole butyric acid(IBA)and gibberellin were found to promote biodegradation of DBT and diesel,respectively.Concentrations of plant hormones,pH,temperature,soil moisture and substrate concentrations were optimized in microbial metabolic processes.Two main factors including temperature and IBA concentration were determined by factor analysis in DBT biodegradation.And soil moisture and diesel concentration were important factors in diesel biodegradation.Binding sites between cell surface and DBT or diesel components were performed by molecular operating environment(MOE).This study suggested that plant hormones could be applied to effectively remove pollutants in environment.