Rupi Sanjie capsule plus surgical operation for the treatment of hyperplasia nodule of mammary gland:A meta-analysis and trial sequential analysis

Objective:To evaluate the efficacy and safety of Rupi Sanjie(RPSJ)capsule plus the conventional surgical operation for treatment of hyperplasia nodule of mammary gland.Methods:A meta-analysis was conducted on randomized controlled trials(RCT)related to RPSJ capsule plus surgical operation in the treatment of hyperplasia nodule of mammary gland.The methodological quality of eligible RCTs was assessed according to the criteria from the Cochrane Handbook for Systematic Reviews of Interventions.RevMan5.3 and Stata14.0 were used for data analyses.Trial sequential analysis was performed to estimate the sample size of systematic review base on TSA software v0.9.Results:Twenty-two RCTs were totally included in this study,involving 2135 patients.The result showed the clinical recurrence rate of RPSJ capsule plus surgical treatment group was significantly lower than surgical treatment alone group(RR:0.25,95%CI[0.17,0.37],P<0.01).The clinical cure rate and total effective rate were higher in RPSJ capsule plus surgical treatment group than the conventional surgical treatment group(RR:1.63,95%CI[1.46,1.82],P<0.01);(RR:1.29,95%CI[1.22,1.37],P<0.01).Application of RPSJ capsule decreased the occurrence of adverse events including nausea,vomiting,irregular menstruation,constipation,dizziness,and headache etc.(RR:0.90,95%CI[0.54,1.49],P=0.68).The results of trial sequential analysis demonstrated that the current available data did not reach the expected value.Conclusion:RPSJ capsule plus the conventional surgical treatment was more effective in reducing the clinical recurrence rate,and improving the total clinical effective rate and clinical cure rate,with a decrease in the occurrence of adverse events.

Mechanism of therapeutic effects of Huangbo on ureteral stones based on network pharmacology

Objective:To analyze the effective chemical components and action targets of Phellodendron chinense,and to study the mechanism of Phellodendron chinense in treating ureteral stones.Methods:Search the potential chemical active substances of Phellodendron chinense and target proteins acting on the human body through the TCMSP database,and use the genomic annotation database platform(Genecards)to predict the target of ureteral stones,and use the uniprot database Query the corresponding gene names,build a network diagram of"drug-disease-target"with the help of Cytoscape(3.7.2)software,build a protein interaction network through the String database platform,and then use the Bioconductor platform and R language GO Enrichment analysis and KEGG enrichment analysis.Results:Through screening,a total of 36 effective chemical constituents of Phellodendron chinense,8 key chemical constituents related to ureteral calculi,and 20 common targets of Phellodendron-ureteral calculi were obtained.The core genes of PPI were FOS,CXCL8,IL6,SERPINE1,VEGFA,EGF,NOS3,SPP1,CCL2,CCND1,EGFR,HMOX1,IL10,CDKN1A,CRP;obtained 36 GO biological processes,and 14 KEGG related signaling pathways,including HIF-1 signaling pathway,FoxO signaling pathway,EGFR tyrosine kinase inhibitor resistance,endocrine resistance,viral protein interaction with cytokines and cytokine receptors,calcium signaling pathway,p53 signaling pathway,ErbB signaling pathway,MAPK signaling pathway,Ras signaling pathway.Conclusion:Phellodendron can achieve the effect of treating ureteral calculi through multiple targets and multiple ways,which provides a theoretical basis for the future extraction of effective components to treat ureteral calculi.