Objective:To explore the anti-gout effects of four traditional Chinese medicine extracts of Eucommia,Sargentodoxa cuneata,Caulis spatholobi and Phellinus igniarius.Methods:Monosodium Urate(MSU)was used to establish a ...Objective:To explore the anti-gout effects of four traditional Chinese medicine extracts of Eucommia,Sargentodoxa cuneata,Caulis spatholobi and Phellinus igniarius.Methods:Monosodium Urate(MSU)was used to establish a mouse gouty arritis model.With coloricine as the positive control group,the effects of four traditional Chinese medicine extracts of Ecuador,Sargentodoxa,Caulis spatholobi and Phellinus igniarius on rice were studied;The hot plate method was used to observe the analytical effects of the four groups of traditional Chinese medicine extracts;the enzyme-linked immunoassay(ELISA)was used to detect residual interference-1 beta(IL-1 beta);the four traditional Chinese medicine extracts were simultaneously determined on the white blood cells,and monocytes in the blood of mice as well as the influence of neutrophils and histopathological changes of toes.Results:Compared with the normal group,the joint diameter ratio,blood leukocytes,monocytes,neutrophils,IL-1βat different time points in the model group were significantly increased(P<0.01),and the pain percentage was significantly reduced(P<0.05).Compared with the model group,the joint diameter ratio of the colchicine group,the Eucommia group,the Sargentodoxa group,the Caulis spatholobi group and the Phellinus sibiricum group was significantly reduced(P<0.05),and the pain percentage was significantly increased(P(0.05).The four groups of traditional Chinese medicine extracts significantly decreased the white blood cells,monocytes,and neutrophils in the blood of mice(P<0.05),and the IL-1βexpression levels of the Eucommia ulmoides group,)the Sargentodoxa group and the Phellinus igniarius group significantly decreased(P<0.05).Eucommia ulmoides and Caulis spatholobi are more effective in reducing pathological damage of toes in mice.Conclusion:The water extract of Caulis spatholobi has obvious anti-gout effect,and the most significant effect on analgesia and swelling.Eucommia ulmoides and Sargentodoxa cuneata have more obvious anti-inflammatory effects and have certain analgesic and swelling effects.Phellinus Phellinus igniarius has obvious inhibitory effect on IL-1β,but it does not show good anti-gout potential.展开更多
基金National Natural Science Foundation of China(No.81960663)2016 Student Innovation Training Program of Hainan Medical University(No.HYCX2016055)+1 种基金2017 College Student Innovation and Entrepreneurship Training Program of Hainan Province(No.2017098)2018 Research Start Funding of Hainan Medical University。
文摘Objective:To explore the anti-gout effects of four traditional Chinese medicine extracts of Eucommia,Sargentodoxa cuneata,Caulis spatholobi and Phellinus igniarius.Methods:Monosodium Urate(MSU)was used to establish a mouse gouty arritis model.With coloricine as the positive control group,the effects of four traditional Chinese medicine extracts of Ecuador,Sargentodoxa,Caulis spatholobi and Phellinus igniarius on rice were studied;The hot plate method was used to observe the analytical effects of the four groups of traditional Chinese medicine extracts;the enzyme-linked immunoassay(ELISA)was used to detect residual interference-1 beta(IL-1 beta);the four traditional Chinese medicine extracts were simultaneously determined on the white blood cells,and monocytes in the blood of mice as well as the influence of neutrophils and histopathological changes of toes.Results:Compared with the normal group,the joint diameter ratio,blood leukocytes,monocytes,neutrophils,IL-1βat different time points in the model group were significantly increased(P<0.01),and the pain percentage was significantly reduced(P<0.05).Compared with the model group,the joint diameter ratio of the colchicine group,the Eucommia group,the Sargentodoxa group,the Caulis spatholobi group and the Phellinus sibiricum group was significantly reduced(P<0.05),and the pain percentage was significantly increased(P(0.05).The four groups of traditional Chinese medicine extracts significantly decreased the white blood cells,monocytes,and neutrophils in the blood of mice(P<0.05),and the IL-1βexpression levels of the Eucommia ulmoides group,)the Sargentodoxa group and the Phellinus igniarius group significantly decreased(P<0.05).Eucommia ulmoides and Caulis spatholobi are more effective in reducing pathological damage of toes in mice.Conclusion:The water extract of Caulis spatholobi has obvious anti-gout effect,and the most significant effect on analgesia and swelling.Eucommia ulmoides and Sargentodoxa cuneata have more obvious anti-inflammatory effects and have certain analgesic and swelling effects.Phellinus Phellinus igniarius has obvious inhibitory effect on IL-1β,but it does not show good anti-gout potential.
