The increase through tyrosinase levels after shrimp death causes enzymatic browning,affecting the appearance and freshness of products,and it is essential to establish a simple and rapid method to detect tyrosinase in...The increase through tyrosinase levels after shrimp death causes enzymatic browning,affecting the appearance and freshness of products,and it is essential to establish a simple and rapid method to detect tyrosinase in the aquatic product supply chain to control the market’s good quality.In this study,we used a constant potential deposition method to create a [(Polystyrene Sodium Sulfonate(PS S)/Polypyrrole(PPy))(P2Mo17Ni/PPy)5] polystyrene sodium sulfonate polymetallic oxide thin film electrodes that can realize the detection of tyrosinase(TYR) in shrimp samples.The method yielded a limit of detection(Signal-Noise ratio(S/N)=3) of 0.1028 U·mL^(-1) within the linear detection of 3.22-26.04 U·mL^(-1),and the recoveries of the samples ranged from 96.37% to 102.40%.The above data confirmed that the method showed some accuracy in the detection of TYR content in shrimps and provided a theoretical basis for the preparation of tyrosinase sensors for the application of polyoxometalates in aquatic products.展开更多
基金the support of the National Natural Science Foundation of China (Grant No. 21871110)the Natural Science Foundation of China Fujian Province (Grant No. 2020J01674)。
文摘The increase through tyrosinase levels after shrimp death causes enzymatic browning,affecting the appearance and freshness of products,and it is essential to establish a simple and rapid method to detect tyrosinase in the aquatic product supply chain to control the market’s good quality.In this study,we used a constant potential deposition method to create a [(Polystyrene Sodium Sulfonate(PS S)/Polypyrrole(PPy))(P2Mo17Ni/PPy)5] polystyrene sodium sulfonate polymetallic oxide thin film electrodes that can realize the detection of tyrosinase(TYR) in shrimp samples.The method yielded a limit of detection(Signal-Noise ratio(S/N)=3) of 0.1028 U·mL^(-1) within the linear detection of 3.22-26.04 U·mL^(-1),and the recoveries of the samples ranged from 96.37% to 102.40%.The above data confirmed that the method showed some accuracy in the detection of TYR content in shrimps and provided a theoretical basis for the preparation of tyrosinase sensors for the application of polyoxometalates in aquatic products.
