In this study, we hypothesized that an increase in integrin αβand its co-activator vascular endothelial growth factor play important neuroprotective roles in ischemic injury. We performed ischemic preconditioning wi...In this study, we hypothesized that an increase in integrin αβand its co-activator vascular endothelial growth factor play important neuroprotective roles in ischemic injury. We performed ischemic preconditioning with bilateral common carotid artery occlusion for 5 minutes in C57BL/6J mice. This was followed by ischemic injury with bilateral common carotid artery occlusion for 30 minutes. The time interval between ischemic preconditioning and lethal ischemia was 48 hours. Histopathological analysis showed that ischemic preconditioning substantially diminished damage to neurons in the hippocampus 7 days after ischemia. Evans Blue dye assay showed that ischemic preconditioning reduced damage to the blood-brain barrier 24 hours after ischemia. This demonstrates the neuroprotective effect of ischemic preconditioning. Western blot assay revealed a significant reduction in protein levels of integrin αβ, vascular endothelial growth factor and its receptor in mice given ischemic preconditioning compared with mice not given ischemic preconditioning 24 hours after ischemia. These findings suggest that the neuroprotective effect of ischemic preconditioning is associated with lower integrin αβand vascular endothelial growth factor levels in the brain following ischemia.展开更多
By using the three-dimensional classical ensemble model,the recollision dynamics in nonsequential double ionization(NSDI)of Ar by 780-nm laser pulses at(6-1.2)×10^(14)W/cm^(2) was extensively studied.We revealed ...By using the three-dimensional classical ensemble model,the recollision dynamics in nonsequential double ionization(NSDI)of Ar by 780-nm laser pulses at(6-1.2)×10^(14)W/cm^(2) was extensively studied.We revealed the picture of multiplerecollision in the double ionization events at the laser intensity region below the recollision-ionization threshold.Via tracing the NSDI trajectories,it was found that the contribution of these multiple-recollision events increases as the laser intensity decreases.In this low intensity region,many multiple-recollision induced NSDI trajectories occur through the doubly excited states.The decay speed of the doubly excited state decreases with the decreasing laser intensity.展开更多
基金supported by grants from the National Natural Science Foundation of China,No.81071068the Israel Science Foundation-the National Natural Science Foundation of China(Joint Program),No.813111290the Natural Science Foundation of Guangdong Province in China,No.2014A030313172
文摘In this study, we hypothesized that an increase in integrin αβand its co-activator vascular endothelial growth factor play important neuroprotective roles in ischemic injury. We performed ischemic preconditioning with bilateral common carotid artery occlusion for 5 minutes in C57BL/6J mice. This was followed by ischemic injury with bilateral common carotid artery occlusion for 30 minutes. The time interval between ischemic preconditioning and lethal ischemia was 48 hours. Histopathological analysis showed that ischemic preconditioning substantially diminished damage to neurons in the hippocampus 7 days after ischemia. Evans Blue dye assay showed that ischemic preconditioning reduced damage to the blood-brain barrier 24 hours after ischemia. This demonstrates the neuroprotective effect of ischemic preconditioning. Western blot assay revealed a significant reduction in protein levels of integrin αβ, vascular endothelial growth factor and its receptor in mice given ischemic preconditioning compared with mice not given ischemic preconditioning 24 hours after ischemia. These findings suggest that the neuroprotective effect of ischemic preconditioning is associated with lower integrin αβand vascular endothelial growth factor levels in the brain following ischemia.
基金Project supported by the Natural Science Foundation of Hubei Province,China(Grant No.2020CFB362)Scientific Research Program of Hubei Provincial Department of Education,China(Grant No.B2020176)the National Natural Science Foundation of China(Grant No.12104389)。
文摘By using the three-dimensional classical ensemble model,the recollision dynamics in nonsequential double ionization(NSDI)of Ar by 780-nm laser pulses at(6-1.2)×10^(14)W/cm^(2) was extensively studied.We revealed the picture of multiplerecollision in the double ionization events at the laser intensity region below the recollision-ionization threshold.Via tracing the NSDI trajectories,it was found that the contribution of these multiple-recollision events increases as the laser intensity decreases.In this low intensity region,many multiple-recollision induced NSDI trajectories occur through the doubly excited states.The decay speed of the doubly excited state decreases with the decreasing laser intensity.
