Effects of Ang Ⅱ perfusion on transmural heterogeneous of Cx43 in acute myocardial ischemia reperfusion

Objective:To observe the effects of angiotensin Ⅱ(Ang Ⅱ) pefusion on transmural heterogeneity of Cx43 expression in the rabbit model with acute myocardial ischemia reperfusion(MIR),and investigate the role of rennin-angiotensin system in malignant ventricular arrhythmia induced by MIR.Methods:Twenty rabbits were randomly divided into MIR group(n=10) and Ang Ⅱ group(n=10).MIR model was produced with traditional ligation and opening of the anterior descending coronary artery in all animal.The hearts in vitro in the MIR group and the Ang Ⅱ group were perfused with simply improved Tyrode's solution and containing Ang Ⅱ Tyrode's solution respectively.90%monophasic action potential repolarization duration,transmural dispersion of repolarization.Cx43 protein(Cx43-pro) and mRNA(Cx43-Cq) expression in subepicardial,midmyocardial and subendocardial myocardium were measured in both groups.The greatest differences of Cx43-pro and Cx43-Cq among three myocardial layers were calculated and shown with △Cx43-pro and △Cx43-Cq respectively.Results:After Ang Ⅱ perfusion,90%monophasic action potential repolarization duration among three myocardial layer were significantly prolonged(P < 0.05 and P < 0.01),and transmural dispersion of repolarization also significantly increased compared with the MIR group(P < 0.05).Compare with the MIR group,three myocardial Cx43-pro and Cx43-Cq expression in the Ang Ⅱ group were significantly decreased(P < 0.05 and P < 0.01).but△Cx43-pro and △Cx43-Cq were significant increased.Conclusions:Renin-angiotensin system increases transmural heterogeneity of Cx43 expression in the rabbit model with MIR by Ang Ⅱ,and enlarge transmural dispersion of repolarization among three myocardial layers of left ventricular which induces malignant ventricular arrhythmia.

Application of individualized nutritional intervention therapy in patients with gestational diabetes mellitu

Objective:To evaluate the effect of individualized nutritional intervention therapy in patients with gestational diabetes mellitus(GDM).Methods:80 GDM inpatients admitted to the First Affiliated Hospital of Hainan Medical College from December 2015 to June 2018 were enrolled.According to the random number table method,the patients were divided into 4 groups,with 20 cases in each group.Group 1 used food exchange combined glycemic index method,group 2 used food exchange combined blood glucose load method,group 3 used glycemic index combined with blood glucose load method,group 4 combined glycemic index and blood glucose load method based on food exchange.After the intervention,the changes of blood glucose level,biochemical indexes and fetal growth and development indexes of the four groups were observed.Results:After nutritional intervention,the blood glucose levels of the four groups of patients decreased to varying degrees compared with before the intervention.The difference was statistically significant(P<0.05).In order to evaluate the advantages and disadvantages of the four methods,the blood glucose levels of the four groups of patients were further compared between groups:compared with group 1,group 2,group 4 and group 3,the differences were statistically significant(P<0.01 or 0.05).There was significant difference in blood glucose 2 hours after dinner between group 4 and group 1 and group 2(P<0.05).Comparing the biochemical indexes and fetal growth and development indexes of the four groups,the differences were not statistically significant(P>0.05).Conclusion:Individualized nutritional intervention therapy can significantly improve the blood glucose level of GDM patients,and at the same time meet the needs of the mother's own physiology and fetal growth and development.Among them,the nutrition intervention method that combines the glycemic index and the blood glucose load on the basis of the food exchange portion has a better effect on improving the blood glucose level of the patient.

Study of MiRNA in autoimmune thyroid diseases

Objective: Recent studies have found that microRNA (miRNA) is a potential novel biomarker, and its abnormal expression is associated with autoimmune thyroid diseases, such as hashimoto's thyroiditis and diffuse toxic goitre. This paper reviews the biological origin of miRNA and the mechanism of miRNAs in autoimmune thyroid diseases.