Objective: To study the significance of E-selectin andits ligand-sLeX in the metastasis of hepatocellularcarcinoma (HCC).Methods: Flow cytometry and immunohistochemistrywere used to detect the expression of E-selectin...Objective: To study the significance of E-selectin andits ligand-sLeX in the metastasis of hepatocellularcarcinoma (HCC).Methods: Flow cytometry and immunohistochemistrywere used to detect the expression of E-selectin and itsligand-sLeX in both HCC cell lines and human HCCtissues.Results: The positive rate of E-selectin in vascular en-dothelial cells adjacent to cancer was 67.9% (19/28). Theexpression of E-selectin in tumors accompanied withemboli or satellite foci was significantly higher than thatin tumors without emboli or satellite foci (P<0.05),and it was not related to tumor size, tumor capsule,AFP content, and the degree of differentiation. Thepositive expression of sLeX in SMMU-7721, PLF/PRF/5 and HepGⅡ cell lines was 7.03%, 63.35% and97.29% respectively. The positive cells of sLeX mainlydistributed in the margin of tumor tissues. The positiveexpression of sLeX in HCC cells in emboli or invasivetumor tissues was much higher than in primary foci.Conclusion: E-selectin and its ligand-sLeX are closelycorrelated with the metastasis of HCC.展开更多
文摘Objective: To study the significance of E-selectin andits ligand-sLeX in the metastasis of hepatocellularcarcinoma (HCC).Methods: Flow cytometry and immunohistochemistrywere used to detect the expression of E-selectin and itsligand-sLeX in both HCC cell lines and human HCCtissues.Results: The positive rate of E-selectin in vascular en-dothelial cells adjacent to cancer was 67.9% (19/28). Theexpression of E-selectin in tumors accompanied withemboli or satellite foci was significantly higher than thatin tumors without emboli or satellite foci (P<0.05),and it was not related to tumor size, tumor capsule,AFP content, and the degree of differentiation. Thepositive expression of sLeX in SMMU-7721, PLF/PRF/5 and HepGⅡ cell lines was 7.03%, 63.35% and97.29% respectively. The positive cells of sLeX mainlydistributed in the margin of tumor tissues. The positiveexpression of sLeX in HCC cells in emboli or invasivetumor tissues was much higher than in primary foci.Conclusion: E-selectin and its ligand-sLeX are closelycorrelated with the metastasis of HCC.
