Objective:To study the effect of Tangshenjiangzhuo granules on serum cytokines as well as the oxidative stress and endoplasmic reticulum stress in kidney tissue of diabetic rats. Methods:SPF male Wistar rats were sele...Objective:To study the effect of Tangshenjiangzhuo granules on serum cytokines as well as the oxidative stress and endoplasmic reticulum stress in kidney tissue of diabetic rats. Methods:SPF male Wistar rats were selected as experimental animals and randomly divided into control group, model group and intervention group. Model group received intragastric administration of saline after diabetes model was established, and the intervention group received intragastric administration of Tangshenjiangzhuo granules after diabetes model was established. 8 weeks after treatment, serum was collected to detect the levels of pro-inflammatory cytokines and adipocytokines, and kidney tissue was collected to detect the levels of oxidative stress molecules and endoplasmic reticulum stress molecules. Results:Serum interleukin-6 (IL-6), macrophage migration inhibitory factor (MIF), regulated upon activation normal T cell expressed secreted (RANTES), Chemerin and Nesfatin levels of model group were significantly higher than those of control group (P<0.05), and serum IL-6, MIF, RANTES, Chemerin and Nesfatin levels of intervention group were significantly lower than those of model group (P<0.05);reactive oxygen species (ROS), malondialdehyde (MDA), advanced oxidation protein products (AOPP), 8-hydroxy-2-deoxyguanosine (8-OHdG), glucose-regulated protein (GRP78), CCAAT/enhancer-binding protein homologous protein (CHOP) and ATF4 protein levels in kidney tissue of model group were significantly higher than those of control group (P<0.05), and ROS, MDA, AOPP, 8-OHdG, GRP78, CHOP and ATF4 levels in kidney tissue of intervention group were significantly lower than model group (P<0.05). Conclusions:Tangshenjiangzhuo granules can adjust the secretion of pro-inflammatory factors and adipocytokines, and inhibit oxidative stress and endoplasmic reticulum stress in kidney tissue of diabetic rat models.展开更多
文摘Objective:To study the effect of Tangshenjiangzhuo granules on serum cytokines as well as the oxidative stress and endoplasmic reticulum stress in kidney tissue of diabetic rats. Methods:SPF male Wistar rats were selected as experimental animals and randomly divided into control group, model group and intervention group. Model group received intragastric administration of saline after diabetes model was established, and the intervention group received intragastric administration of Tangshenjiangzhuo granules after diabetes model was established. 8 weeks after treatment, serum was collected to detect the levels of pro-inflammatory cytokines and adipocytokines, and kidney tissue was collected to detect the levels of oxidative stress molecules and endoplasmic reticulum stress molecules. Results:Serum interleukin-6 (IL-6), macrophage migration inhibitory factor (MIF), regulated upon activation normal T cell expressed secreted (RANTES), Chemerin and Nesfatin levels of model group were significantly higher than those of control group (P<0.05), and serum IL-6, MIF, RANTES, Chemerin and Nesfatin levels of intervention group were significantly lower than those of model group (P<0.05);reactive oxygen species (ROS), malondialdehyde (MDA), advanced oxidation protein products (AOPP), 8-hydroxy-2-deoxyguanosine (8-OHdG), glucose-regulated protein (GRP78), CCAAT/enhancer-binding protein homologous protein (CHOP) and ATF4 protein levels in kidney tissue of model group were significantly higher than those of control group (P<0.05), and ROS, MDA, AOPP, 8-OHdG, GRP78, CHOP and ATF4 levels in kidney tissue of intervention group were significantly lower than model group (P<0.05). Conclusions:Tangshenjiangzhuo granules can adjust the secretion of pro-inflammatory factors and adipocytokines, and inhibit oxidative stress and endoplasmic reticulum stress in kidney tissue of diabetic rat models.
