Objective: To observe the expression and significance of fibronectin and metalloproteinase-3(MMP-3) in patients with ankylosing spondylitis(AS). Methods: A total of 30 AS patients in our hospital and 30 healthy volunt...Objective: To observe the expression and significance of fibronectin and metalloproteinase-3(MMP-3) in patients with ankylosing spondylitis(AS). Methods: A total of 30 AS patients in our hospital and 30 healthy volunteers were selected in our study. Fibronectin and MMP-3 were measured and compared between these two groups. The AS group received sulfasalazine2 g daily for 3 months. Bath ankylosing spondylitis disease activity index, bath ankylosing spondylitis functional index, bath ankylosing spondylitis metrology index, erythrocyte sedimentation rate and C-reactive protein were compared before and after treatment.Pearson's linear-correlation analysis was used to determine relationships between parameters.Results: Totally 28 patients in the AS group completed the study. Fibronectin and MMP-3 in peripheral blood of AS patients were evidently higher than that in the normal control group(P<0.05). After treated by sulfasalazine, the level of expressing Fibronectin and MMP-3 significantly decreased compared with baseline values(P<0.05). Pearson's linear-correlation analysis showed that serum fibronectin and MMP-3 level had a positive correlation with bath ankylosing spondylitis disease activity index global assessment, spine pain, night pain, general pain, erythrocyte sedimentation rate and C-reactive protein(P<0.05). Conclusions: The expression of fibronectin and MMP-3 in AS patients were significantly higher than that in the normal control group, and they all decreased significantly after treatment. It indicated that both fibronectin and MMP-3 were correlated closely with the onset of AS.展开更多
In this paper,we design and fabricate a novel three-dimensional passive-micro-mixer by using Polymethyl methacrylate( PMMA). The mixer is fabricated by using ultra-precision engraving machine and bonded by an organic ...In this paper,we design and fabricate a novel three-dimensional passive-micro-mixer by using Polymethyl methacrylate( PMMA). The mixer is fabricated by using ultra-precision engraving machine and bonded by an organic solvent fumigation bonding method. The mixer combines two fluid streams into a mixing chamber integrated with T-shaped pre-mixing and six tortuous shaped mixing elements. We have employed three-dimensional numerical simulations to evaluate the mixing efficiency. The simulation results indicate,under inlet fluid pressure p = 10( Pa), compared to the planar serpentine mixer and tortuous mixer,the concentration fluctuation range at the outlet diagonal reduce to 48% and 71. 6% respectively. And the mixing concentration variances also show that the mixing efficiency has a significant increase. We characterize the device by using visualization microscope,and the results are consistent with the simulation data. The device demonstrates the promising capabilities for micro total analysis system integration.展开更多
基金supported by Chinese Medicine Research Program of Zhejiang Province(No.2015ZB121)
文摘Objective: To observe the expression and significance of fibronectin and metalloproteinase-3(MMP-3) in patients with ankylosing spondylitis(AS). Methods: A total of 30 AS patients in our hospital and 30 healthy volunteers were selected in our study. Fibronectin and MMP-3 were measured and compared between these two groups. The AS group received sulfasalazine2 g daily for 3 months. Bath ankylosing spondylitis disease activity index, bath ankylosing spondylitis functional index, bath ankylosing spondylitis metrology index, erythrocyte sedimentation rate and C-reactive protein were compared before and after treatment.Pearson's linear-correlation analysis was used to determine relationships between parameters.Results: Totally 28 patients in the AS group completed the study. Fibronectin and MMP-3 in peripheral blood of AS patients were evidently higher than that in the normal control group(P<0.05). After treated by sulfasalazine, the level of expressing Fibronectin and MMP-3 significantly decreased compared with baseline values(P<0.05). Pearson's linear-correlation analysis showed that serum fibronectin and MMP-3 level had a positive correlation with bath ankylosing spondylitis disease activity index global assessment, spine pain, night pain, general pain, erythrocyte sedimentation rate and C-reactive protein(P<0.05). Conclusions: The expression of fibronectin and MMP-3 in AS patients were significantly higher than that in the normal control group, and they all decreased significantly after treatment. It indicated that both fibronectin and MMP-3 were correlated closely with the onset of AS.
基金Sponsored by the Natural Science Foundation of Heilongjiang Province(Grant No.F201007)the Fundamental Research Funds for the Central Universities(Grant No.HIT.NSRIF.2010077)
文摘In this paper,we design and fabricate a novel three-dimensional passive-micro-mixer by using Polymethyl methacrylate( PMMA). The mixer is fabricated by using ultra-precision engraving machine and bonded by an organic solvent fumigation bonding method. The mixer combines two fluid streams into a mixing chamber integrated with T-shaped pre-mixing and six tortuous shaped mixing elements. We have employed three-dimensional numerical simulations to evaluate the mixing efficiency. The simulation results indicate,under inlet fluid pressure p = 10( Pa), compared to the planar serpentine mixer and tortuous mixer,the concentration fluctuation range at the outlet diagonal reduce to 48% and 71. 6% respectively. And the mixing concentration variances also show that the mixing efficiency has a significant increase. We characterize the device by using visualization microscope,and the results are consistent with the simulation data. The device demonstrates the promising capabilities for micro total analysis system integration.
基金Project supported by the National Natural Science Foundation of China(Nos.81171472 and 81201407)the Innovation Team Project of Sichuan Provincial Education Department(No.13TD0030)+1 种基金the Major Transformation Cultivation Project of Sichuan Provincial Education Department(No.15CZ0021)the Science and Technology Project of Nanchong City(No.14A0021),China