Dental caries （tooth decay） is caused by a specific group of cariogenic bacteria, like Streptococcus mutans, which convert dietary sugars into acids that dissolve the mineraI in tooth structure. Killing cariogenic b...Dental caries （tooth decay） is caused by a specific group of cariogenic bacteria, like Streptococcus mutans, which convert dietary sugars into acids that dissolve the mineraI in tooth structure. Killing cariogenic bacteria is an effective way to control or prevent tooth decay. In a previous study, we discovered a novel compound （Glycyrrhizol A）, from the extraction of licorice roots, with strong antimicrobial activity against cariogenic bacteria. In the current study, we developed a method to produce these specific herbal extracts in large quantities, and then used these extracts to develop a sugar-free lollipop that effectively kills cariogenic bacteria like Streptococcus mutans. Further studies showed that these sugar-free lollipops are safe and their antimicrobial activity is stable. Two pilot human studies indicate that a brief application of these lollipops （twice a day for ten days） led to a marked reduction of cariogenic bacteria in oral cavity among most human subjects tested. This herbal lollipop could be a novel tool to promote oral health through functional foods.展开更多
Although implantation of biomaterials carrying mesenchymal stem cells(MSCs)is considered as a promising strategy for ameliorating neural function after spinal cord injury(SCI),there are still some challenges including...Although implantation of biomaterials carrying mesenchymal stem cells(MSCs)is considered as a promising strategy for ameliorating neural function after spinal cord injury(SCI),there are still some challenges including poor cell survival rate,tumorigenicity and ethics concerns.The performance of the secretome derived from MSCs was more stable,and its clinical transformation was more operable.Cytokine antibody array demonstrated that the secretome of MSCs contained 79 proteins among the 174 proteins analyzed.Three-dimensional(3D)printed collagen/silk fibroin scaffolds carrying MSCs secretome improved hindlimb locomotor function according to the Basso–Beattie–Bresnahan scores,the inclined-grid climbing test and electrophysiological analysis.Parallel with locomotor function recovery,3D printed collagen/silk fibroin scaffolds carrying MSCs secretome could further facilitate nerve fiber regeneration,enhance remyelination and accelerate the establishment of synaptic connections at the injury site compared to 3D printed collagen/silk fibroin scaffolds alone group according to magnetic resonance imaging,diffusion tensor imaging,hematoxylin and eosin staining,Bielschowsky’s silver staining,immunofluorescence staining and transmission electron microscopy.These results indicated the implantation of 3D printed collagen/silk fibroin scaffolds carrying MSCs secretome might be a potential treatment for SCI.展开更多
基金supported by C3 Jian Inc, Delta Dental-California and UCLA Oppenheimer Award
文摘Dental caries （tooth decay） is caused by a specific group of cariogenic bacteria, like Streptococcus mutans, which convert dietary sugars into acids that dissolve the mineraI in tooth structure. Killing cariogenic bacteria is an effective way to control or prevent tooth decay. In a previous study, we discovered a novel compound （Glycyrrhizol A）, from the extraction of licorice roots, with strong antimicrobial activity against cariogenic bacteria. In the current study, we developed a method to produce these specific herbal extracts in large quantities, and then used these extracts to develop a sugar-free lollipop that effectively kills cariogenic bacteria like Streptococcus mutans. Further studies showed that these sugar-free lollipops are safe and their antimicrobial activity is stable. Two pilot human studies indicate that a brief application of these lollipops （twice a day for ten days） led to a marked reduction of cariogenic bacteria in oral cavity among most human subjects tested. This herbal lollipop could be a novel tool to promote oral health through functional foods.
基金supported by the National Key Research and Development Plan of China(2021YFF1200800)the National Nature Scientific Fund of China(82171861,81771352,81971782,81671222,81771350)the Nature Scientific Fund of Tianjin(18JCJQJC48500,19JCYBJC27900).
文摘Although implantation of biomaterials carrying mesenchymal stem cells(MSCs)is considered as a promising strategy for ameliorating neural function after spinal cord injury(SCI),there are still some challenges including poor cell survival rate,tumorigenicity and ethics concerns.The performance of the secretome derived from MSCs was more stable,and its clinical transformation was more operable.Cytokine antibody array demonstrated that the secretome of MSCs contained 79 proteins among the 174 proteins analyzed.Three-dimensional(3D)printed collagen/silk fibroin scaffolds carrying MSCs secretome improved hindlimb locomotor function according to the Basso–Beattie–Bresnahan scores,the inclined-grid climbing test and electrophysiological analysis.Parallel with locomotor function recovery,3D printed collagen/silk fibroin scaffolds carrying MSCs secretome could further facilitate nerve fiber regeneration,enhance remyelination and accelerate the establishment of synaptic connections at the injury site compared to 3D printed collagen/silk fibroin scaffolds alone group according to magnetic resonance imaging,diffusion tensor imaging,hematoxylin and eosin staining,Bielschowsky’s silver staining,immunofluorescence staining and transmission electron microscopy.These results indicated the implantation of 3D printed collagen/silk fibroin scaffolds carrying MSCs secretome might be a potential treatment for SCI.
