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SAPT:a synchrotron-based proton therapy facility in Shanghai 被引量:2
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作者 Man-Zhou Zhang De-Ming Li +45 位作者 Li-Ren Shen Hai-Rong Zhang Zhi-Ling Chen Han-Wen Du Ming Gu Rui Li De-Kang Liu Yue-Hu Pu Jun-Feng Yu Jian-Feng Chen Chu Chen Chun-Long Guo Hao Guo Ge-Yang Jiang Zhi-Qiang Jiang Lin Jin Wen-Jing Li Xiu-Fang Li Ye Lin Ming Liu Yong-Hao Liu Ya-Juan Liu Ming Lv Qing-Ru Mi Lian-Hua Ouyang Wei-Guo Shi Hang Shu Qi-Sheng Tang Kun Wang Zhi-Shan Wang Jun wu xiao-bing wu Jia-Qiang Xu Wen-Zhen Xu Chong-Xian Yin Cheng Yu Ren-Xian Yuan Qi-Bing Yuan Hai-Qun Zhang Miao Zhang Wen-Zhi Zhang Li-Ying Zhao Wei-Min Zhou Shou-Xian Fang Xi-Dong Sun Zhen-Tang Zhao 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2023年第10期36-46,共11页
Because of its excellent dose distribution,proton therapy is becoming increasingly popular in the medical application of cancer treatment.A synchrotron-based proton therapy facility was designed and constructed in Sha... Because of its excellent dose distribution,proton therapy is becoming increasingly popular in the medical application of cancer treatment.A synchrotron-based proton therapy facility was designed and constructed in Shanghai.The synchrotron,beam delivery system,and other technical systems were commissioned and reached their expected performances.After a clinical trial of 47 patients was finished,the proton therapy facility obtained a registration certificate from the National Medical Products Administration.The characteristics of the accelerator and treatment systems are described in this article. 展开更多
关键词 Proton therapy SYNCHROTRON Slow extraction GANTRY Beam delivery system
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Evaluation of trabecular meshwork-specific promoters in vitro and in vivo using scAAV2 vectors expressing C3 transferase 被引量:2
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作者 Jun-Kai Tan Ying Xiao +9 位作者 Guo Liu Long-Xiang Huang Wen-Hao Ma Yan Xia Xi-Zhen Wang Xian-Jun Zhu Su-Ping Cai xiao-bing wu Yun Wang Xu-Yang Liu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第8期1196-1209,共14页
AIM:To evaluate the potential of two trabecular meshwork(TM)-specific promoters,Chitinase 3-like 1(Ch3L1)and matrix gla protein(MGP),for improving specificity and safety in glaucoma gene therapy based on self-compleme... AIM:To evaluate the potential of two trabecular meshwork(TM)-specific promoters,Chitinase 3-like 1(Ch3L1)and matrix gla protein(MGP),for improving specificity and safety in glaucoma gene therapy based on self-complementary AAV2(scAAV2)vector technologies.METHODS:An scAAV2 vector with C3 transferase(C3)as the reporter gene(scAAV2-C3)was selected.The scAAV2-C3 vectors were driven by Ch3L1(scAAV2-Ch3L1-C3),MGP(scAAV2-MGP-C3),enhanced MGP(scAAV2-eMGP-C3)and cytomegalovirus(scAAV2-CMV-C3),respectively.The cultured primary human TM cells were treated with each vector at different multiplicities of infections.Changes in cell morphology were observed by phase contrast microscopy.Actin stress fibers and Rho GTPases/Rho-associated protein kinase pathway-related molecules were assessed by immunofluorescence staining,real-time quantitative polymerase chain reaction and Western blot.Each vector was injected intracamerally into the one eye of each rat at low and high doses respectively.In vivo green fluorescence was visualized by a Micron III Retinal Imaging Microscope.Intraocular pressure(IOP)was monitored using a rebound tonometer.Ocular responses were evaluated by slit-lamp microscopy.Ocular histopathology analysis was examined by hematoxylin and eosin staining.RESULTS:In TM cell culture studies,the vectormediated C3 expression induced morphologic changes,disruption of actin cytoskeleton and reduction of fibronectin expression in TM cells by inhibiting the Rho GTPases/Rhoassociated protein kinase signaling pathway.At the same dose,these changes were significant in TM cells treated with scAAV2-CMV-C3 or scAAV2-Ch3L1-C3,but not in cells treated with scAAV2-eMGP-C3 or scAAV2-MGP-C3.At lowinjected dose,the IOP was significantly decreased in the scAAV2-Ch3L1-C3-injected eyes but not in scAAV2-MGPC3-injected and scAAV2-eMGP-C3-injected eyes.At highinjected dose,significant IOP reduction was observed in the scAAV2-eMGP-C3-injected eyes but not in scAAV2-MGP-C3-injected eyes.Similar to scAAV2-CMV-C3,scAAV2-Ch3L1-C3 vector showed efficient transduction both in the TM and corneal endothelium.In anterior segment tissues of scAAV2-eMGP-C3-injected eyes,no obvious morphological changes were found except for the TM.Inflammation was absent.CONCLUSION:In scAAV2-transduced TM cells,the promoter-driven efficiency of Ch3L1 is close to that of cytomegalovirus,but obviously higher than that of MGP.In the anterior chamber of rat eye,the transgene expression pattern of scAAV2 vector is presumably affected by MGP promoter,but not by Ch3L1 promoter.These findings would provide a useful reference for improvement of specificity and safety in glaucoma gene therapy using scAAV2 vector. 展开更多
关键词 self-complementary AAV2 chitinase 3-like 1 matrix gla protein trabecular meshwork C3 transferase
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A simple taurocholate-induced model of severe acute pancreatitis in rats 被引量:15
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作者 Zhong-Hui Liu Jun-Sheng Peng +6 位作者 Chu-Jun Li Zu-Li Yang Jun Xiang Hu Song xiao-bing wu Jun-Rong Chen De-Chang Diao 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第45期5732-5739,共8页
AIM: To investigate gut barrier damage and intestinal bacteria translocation in severe acute pancreatitis (SAP), a simple rat model of SAP was induced and studied.METHODS: Pancreatitis was induced by uniformly distrib... AIM: To investigate gut barrier damage and intestinal bacteria translocation in severe acute pancreatitis (SAP), a simple rat model of SAP was induced and studied.METHODS: Pancreatitis was induced by uniformly distributed injection of 3.8% Na taurocholate (1 mL/kg) beneath the pancreatic capsule. Rats in the control group were injected with normal saline in the identical location. RESULTS: Serum amylase, plasma endotoxin, intestinal permeability, and pancreatitis pathology scores were all markedly higher in the pancreatitis group than in the control group (P < 0.01). The bacterial infection rate was signif icantly higher in the SAP group than in the control group (P < 0.01), observed in parallel by both bacterial culture and real-time polymerase chain reaction. Acute damage of the pancreas was observed histologically in SAP rats, showing interstitial edema, leukocyte infiltration, acinar cell necrosis and hemorrhage. The microstructure of the intestinal mucosa of SAP ratsappeared to be destroyed with loose, shortened microvilli and rupture of the intercellular junction, as shown by electron microscopy. CONCLUSION: Significant gut barrier damage and intestinal bacterial translocation were def initely observed with few potential study confounders in this SAP rat model, suggesting that it may be an appropriate animal model for study of gut barrier damage and bacterial translocation in SAP. 展开更多
关键词 Acute pancreatitis Bacterial translocation INFLAMMATION Real-time polymerase chain reaction
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Scientific footprints: In memory of crocodile expert,Professor Bi-Hui Chen(1931–2022)
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作者 xiao-bing wu 《Zoological Research》 SCIE CAS CSCD 2022年第6期923-926,共4页
At 10:00 am on 28 August 2022,we received the sad news that our beloved supervisor,Professor Bi-Hui Chen(陈壁辉),a world-renowned expert on the Chinese alligator(Alligator sinensis),passed away from multiple organ fai... At 10:00 am on 28 August 2022,we received the sad news that our beloved supervisor,Professor Bi-Hui Chen(陈壁辉),a world-renowned expert on the Chinese alligator(Alligator sinensis),passed away from multiple organ failure at the age of 91(Figure 1).Professor Bi-Hui Chen was born in September 1931 in Pinghe County,Fujian Province,China. 展开更多
关键词 SINENSIS Figure EXPERT
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Optimization of extraction of phenolics from leaves of Ficus virens 被引量:3
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作者 Xiao-xin CHEN xiao-bing wu +4 位作者 Wei-ming CHAI Hui-ling FENG Yan SHI Han-tao ZHOU Qing-xi CHEN 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2013年第10期903-915,共13页
In this research,the conditions for extraction of phenolics from leaves of Ficus virens were optimized using response surface methodology(RSM).The extraction abilities of phenolics(EAP) and flavonoids(EAF),the 2,2-dip... In this research,the conditions for extraction of phenolics from leaves of Ficus virens were optimized using response surface methodology(RSM).The extraction abilities of phenolics(EAP) and flavonoids(EAF),the 2,2-diphenyl-1-pierylhydrazyl(DPPH) free-radical scavenging potential,and the ferric reducing/antioxidant power(FRAP) were used as quality indicators.The results of single-factor experiments showed that temperature,ethanol concentration,extraction time,and the number of extraction cycles were the main influencing variables,and these provided key information for the central composite design.The results of RSM fitted well to a second degree polynomial model and more than 98% of the variability was explained.The ideal extraction conditions for EAP,EAF,DPPH free-radical scavenging potential,and FRAP were obtained.Considering the four quality indicators overall,the ideal extraction conditions were 58% ethanol at 57 °C for 37 min with three extraction cycles.At the ideal extraction conditions,the values of EAP,EAF,DPPH free-radical scavenging potential,and FRAP were 5.72%,3.09%,58.88 mg ascorbic acid equivalent(AAE)/g dry weight(DW),and 15.86 mg AAE/g DW,respectively.In addition,linear correlations were observed between EAP,EAF,and antioxidant potential. 展开更多
关键词 Ficus virens Phenolics FLAVONOIDS ANTIOXIDANTS Response surface methodology(RSM)
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Minimum Time Extrema Estimation for Large-Scale Radio-Frequency Identification Systems
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作者 Xiao-Jun Zhu Li-Jie Xu +1 位作者 xiao-bing wu Bing Chen 《Journal of Computer Science & Technology》 SCIE EI CSCD 2020年第5期1099-1114,共16页
We consider the extrema estimation problem in large-scale radio-frequency identification(RFID)systems,where there are thousands of tags and each tag contains a finite value.The objective is to design an extrema estima... We consider the extrema estimation problem in large-scale radio-frequency identification(RFID)systems,where there are thousands of tags and each tag contains a finite value.The objective is to design an extrema estimation protocol with the minimum execution time.Because the standard binary search protocol wastes much time due to inter-frame overhead,we propose a parameterized protocol and treat the number of slots in a frame as an unknown parameter.We formulate the problem and show how to find the best parameter to minimize the worst-case execution time.Finally,we propose two rules to further reduce the execution time.The first is to find and remove redundant frames.The second is to concatenate a frame from minimum value estimation with a frame from maximum value estimation to reduce the total number of frames.Simulations show that,in a typical scenario,the proposed protocol reduces execution time by 79%compared with the standard binary search protocol. 展开更多
关键词 radio-frequency identification(RFID)system maximum value estimation minimum value estimation time efficient protocol
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