Objective Berberine(BBR)has emerged as a promising therapeutic agent for nonalcoholic fatty liver disease(NAFLD).This study aims to elucidate the underlying molecular mechanisms.Methods In this study,db/db mice were c...Objective Berberine(BBR)has emerged as a promising therapeutic agent for nonalcoholic fatty liver disease(NAFLD).This study aims to elucidate the underlying molecular mechanisms.Methods In this study,db/db mice were chosen as an animal model for NAFLD.A total of 10 healthy C57BL/6J mice and 30 db/db mice were randomly allocated to one of 4 groups:the normal control(NC)group,the diabetic control(DC)group,the Metformin(MET)therapy group,and the BBR therapy group.The total cholesterol(TC),triacylglycerol(TG),low-density lipoprotein cholesterol(LDL-c),high-density lipoprotein cholesterol(HDL-c),aspartate aminotransferase(AST)and alanine aminotransferase(ALT)levels in the serum were measured.The glutathione peroxidase(GSH-Px),glutathione(GSH),malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT),interleukin(IL)-1β,tumor necrosis factor(TNF)-αand monocyte chemotactic protein 1(MCP-1)levels in liver tissue were measured.Hematoxylin and eosin(H&E),acid-Schiff(PAS)and TUNEL stanning was performed for histopathological analysis.Western blotting and immunohistochemistry were conducted to detect the expression levels of key proteins in the AMPK/SIRT1 pathway.Results BBR could improve lipid metabolism,attenuate hepatic steatosis and alleviate liver injury significantly.The excessive oxidative stress,high levels of inflammation and abnormal apoptosis in db/db mice were reversed after BBR intervention.BBR clearly changed the expression of AMP-activated protein kinase(AMPK)/Sirtuin 1(SIRT1),and their downstream proteins.Conclusion BBR could reverse NAFLD-related liver injury,likely by activating the AMPK/SIRT1 signaling pathway to inhibit oxidative stress,inflammation and apoptosis in hepatic tissue.展开更多
High internal phase emulsions (HIPEs) stabilized by nanoparticles based on biomacromolecules are challenging issues in recent decade.Herein,a newly developed HIPE was investigated by using heat-denatured porcine plasm...High internal phase emulsions (HIPEs) stabilized by nanoparticles based on biomacromolecules are challenging issues in recent decade.Herein,a newly developed HIPE was investigated by using heat-denatured porcine plasma protein (PPP) nanoparticles at pH 6.5 as emulsifier,and its emulsifying stability could be significantly enhanced by compounding carrageenan (CG).In the miscible system,PPP and CG formed hybrid particles through non-covalent interaction,and the sizes and zeta-potentials of the particles increased significantly along with addition of CG (from 0 to 0.7%,w/v),reached up to about 3.6 μm and −53 mV at 0.5% (w/v),respectively.CG weakened the ability of PPP to lower interfacial tension of oil/water (O/W),but increased the apparent viscosity of the system.The results from CLSM,rheology and stability experiments indicated a significant increasing trend of the HIPEs stability and solid-like characteristics along with addition of CG.Compared with the controls including bovine serum albumin (BSA),BSA-CG and CG alone,PPP-CG hybrid particles had good performance in fabricating and stabilizing the HIPEs.The work revealed the novel function of PPP as emulsifier of HIPEs and so offered the theoretical direction for application of PPP as a mass by-product,as well as an excellent HIPEs system for food,medicine and cosmetics fields.展开更多
基金supported by grants from the National Natural Science Foundation of China(No.81803799)Hubei Province Natural Science Foundation of China(No.2022CFB092).
文摘Objective Berberine(BBR)has emerged as a promising therapeutic agent for nonalcoholic fatty liver disease(NAFLD).This study aims to elucidate the underlying molecular mechanisms.Methods In this study,db/db mice were chosen as an animal model for NAFLD.A total of 10 healthy C57BL/6J mice and 30 db/db mice were randomly allocated to one of 4 groups:the normal control(NC)group,the diabetic control(DC)group,the Metformin(MET)therapy group,and the BBR therapy group.The total cholesterol(TC),triacylglycerol(TG),low-density lipoprotein cholesterol(LDL-c),high-density lipoprotein cholesterol(HDL-c),aspartate aminotransferase(AST)and alanine aminotransferase(ALT)levels in the serum were measured.The glutathione peroxidase(GSH-Px),glutathione(GSH),malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT),interleukin(IL)-1β,tumor necrosis factor(TNF)-αand monocyte chemotactic protein 1(MCP-1)levels in liver tissue were measured.Hematoxylin and eosin(H&E),acid-Schiff(PAS)and TUNEL stanning was performed for histopathological analysis.Western blotting and immunohistochemistry were conducted to detect the expression levels of key proteins in the AMPK/SIRT1 pathway.Results BBR could improve lipid metabolism,attenuate hepatic steatosis and alleviate liver injury significantly.The excessive oxidative stress,high levels of inflammation and abnormal apoptosis in db/db mice were reversed after BBR intervention.BBR clearly changed the expression of AMP-activated protein kinase(AMPK)/Sirtuin 1(SIRT1),and their downstream proteins.Conclusion BBR could reverse NAFLD-related liver injury,likely by activating the AMPK/SIRT1 signaling pathway to inhibit oxidative stress,inflammation and apoptosis in hepatic tissue.
基金supported by the National Natural Science Foundation of China(31371741).
文摘High internal phase emulsions (HIPEs) stabilized by nanoparticles based on biomacromolecules are challenging issues in recent decade.Herein,a newly developed HIPE was investigated by using heat-denatured porcine plasma protein (PPP) nanoparticles at pH 6.5 as emulsifier,and its emulsifying stability could be significantly enhanced by compounding carrageenan (CG).In the miscible system,PPP and CG formed hybrid particles through non-covalent interaction,and the sizes and zeta-potentials of the particles increased significantly along with addition of CG (from 0 to 0.7%,w/v),reached up to about 3.6 μm and −53 mV at 0.5% (w/v),respectively.CG weakened the ability of PPP to lower interfacial tension of oil/water (O/W),but increased the apparent viscosity of the system.The results from CLSM,rheology and stability experiments indicated a significant increasing trend of the HIPEs stability and solid-like characteristics along with addition of CG.Compared with the controls including bovine serum albumin (BSA),BSA-CG and CG alone,PPP-CG hybrid particles had good performance in fabricating and stabilizing the HIPEs.The work revealed the novel function of PPP as emulsifier of HIPEs and so offered the theoretical direction for application of PPP as a mass by-product,as well as an excellent HIPEs system for food,medicine and cosmetics fields.