Astaxanthin（AST）, a carotenoid molecule extensively found in marine organisms and increasingly used as a dietary supplement, has been reported to have beneficial effects against oxidative stress. In the current pape...Astaxanthin（AST）, a carotenoid molecule extensively found in marine organisms and increasingly used as a dietary supplement, has been reported to have beneficial effects against oxidative stress. In the current paper, the effects of AST on viability of prostate cells were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide（MTT） assay; cell apoptosis and intracellular reactive oxygen species（ROS） levels were determined by flow cytometry; the mitochondrial membrane potential（MMP） was measured by fluorospectrophotometer; and activities of malondialdehyde（MDA）, superoxide dismutase（SOD）, catalase（CAT）, and glutathione peroxidase（GSH-Px） were evaluated by a detection kit. The results show that copper ion（Cu^2＋） induced apoptosis, along with the accumulation of intracellular ROS and MDA, in both prostate cell lines（RWPE-1 and PC-3）. AST treatments could decrease the MDA levels, increase MMP, and keep ROS stable in RWPE-1 cell line. An addition of AST decreased the SOD, GSH-Px, and CAT activities in PC-3 cell line treated with Cu^2＋, but had a contrary reaction in RWPE-1 cell lines. In conclusion, AST could contribute to protecting RWPE-1 cells against Cu^2＋-induced injuries but could cause damage to the antioxidant enzyme system in PC-3 cells.展开更多
基金Project supported by the Plan of Medicine and Health Science and Technology of the Zhejiang Province of China(No.2012RCA022)
文摘Astaxanthin（AST）, a carotenoid molecule extensively found in marine organisms and increasingly used as a dietary supplement, has been reported to have beneficial effects against oxidative stress. In the current paper, the effects of AST on viability of prostate cells were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide（MTT） assay; cell apoptosis and intracellular reactive oxygen species（ROS） levels were determined by flow cytometry; the mitochondrial membrane potential（MMP） was measured by fluorospectrophotometer; and activities of malondialdehyde（MDA）, superoxide dismutase（SOD）, catalase（CAT）, and glutathione peroxidase（GSH-Px） were evaluated by a detection kit. The results show that copper ion（Cu^2＋） induced apoptosis, along with the accumulation of intracellular ROS and MDA, in both prostate cell lines（RWPE-1 and PC-3）. AST treatments could decrease the MDA levels, increase MMP, and keep ROS stable in RWPE-1 cell line. An addition of AST decreased the SOD, GSH-Px, and CAT activities in PC-3 cell line treated with Cu^2＋, but had a contrary reaction in RWPE-1 cell lines. In conclusion, AST could contribute to protecting RWPE-1 cells against Cu^2＋-induced injuries but could cause damage to the antioxidant enzyme system in PC-3 cells.
