The method of nonlinear finite element reliability analysis (FERA) of slope stability using the technique of slip surface stress analysis (SSA) is studied. The limit state function that can consider the direction of s...The method of nonlinear finite element reliability analysis (FERA) of slope stability using the technique of slip surface stress analysis (SSA) is studied. The limit state function that can consider the direction of slip surface is given, and the formula-tions of FERA based on incremental tangent stiffness method and modified Aitken accelerating algorithm are developed. The limited step length iteration method (LSLIM) is adopted to calculate the reliability index. The nonlinear FERA code using the SSA technique is developed and the main flow chart is illustrated. Numerical examples are used to demonstrate the efficiency and robustness of this method. It is found that the accelerating convergence algorithm proposed in this study proves to be very efficient for it can reduce the iteration number greatly, and LSLIM is also efficient for it can assure the convergence of the iteration of the reliability index.展开更多
Testicular endothelial cells have been found to play an important role in spermatogenesis and fertility,but their mechanism is obscure.Exosomes released by various cells are recognized as cell–cell communication medi...Testicular endothelial cells have been found to play an important role in spermatogenesis and fertility,but their mechanism is obscure.Exosomes released by various cells are recognized as cell–cell communication mediators during the initiation and progression of many diseases.Therefore,the current study aimed to investigate the protein and miRNA components of human testicular endothelial cell-derived exosomes(HTEC-Exos)and to explore their potential effects on spermatogenesis.In this study,HTEC-Exos were first isolated by the ultracentrifugation method,and then identified by nanoparticle tracking analysis,transmission electron microscopy(TEM),and western blotting.The characteristics of HTEC-Exos were examined by liquid chromatography–mass spectrometry and microRNA(miRNA)chip analysis.Bioinformatics analysis was performed to explore the potential role of the exosomal content on spermatogenesis.A total of 945 proteins were identified,11 of which were closely related to spermatogenesis.A total of 2578 miRNAs were identified.Among them,30 miRNAs demonstrated potential associations with male reproductive disorders,such as azoospermia,and spermatogenesis disorders.In particular,11 out of these 30 miRNAs have been proven to be involved in spermatogenesis based on available evidence.This study provides a global view of the proteins and miRNAs from HTEC-Exos,suggesting that HTEC-Exos may function as potential effectors during the process of spermatogenesis.展开更多
In engineering practice,the stability of a slope is often analyzed as a 2D problem assuming a plane-strain condition,which may lead to significant errors.In this paper,a comprehensive study is carried out to compare t...In engineering practice,the stability of a slope is often analyzed as a 2D problem assuming a plane-strain condition,which may lead to significant errors.In this paper,a comprehensive study is carried out to compare the results of 2D and 3D slope stability analyses,using the strength reduction method for deterministic analysis and the random field approach for probabilistic analysis,respectively.The results of this comparison study confirm that in the deterministic stability evaluation,the 2D analysis tends to obtain a smaller factor of safety than does its 3D counterpart.In the probabilistic evaluation that considers the spatial variability of soil properties,the 2D analysis tends to yield a larger probability of failure than its 3D counterpart.A significant feature of the 3D probabilistic slope stability analysis is the presence of multiple local failures distributed along the slope longitudinal direction.This paper provides insights regarding the degree of errors in modeling a 3D slope as a 2D problem,which can be regarded as a complement to the previous 3D slope stability analyses.展开更多
基金supported by the National Natural Science Foundation of China (No. 50748033)the Specific Foundation for PhD of Hefei University of Technology (No. 2007GDBJ044), China
文摘The method of nonlinear finite element reliability analysis (FERA) of slope stability using the technique of slip surface stress analysis (SSA) is studied. The limit state function that can consider the direction of slip surface is given, and the formula-tions of FERA based on incremental tangent stiffness method and modified Aitken accelerating algorithm are developed. The limited step length iteration method (LSLIM) is adopted to calculate the reliability index. The nonlinear FERA code using the SSA technique is developed and the main flow chart is illustrated. Numerical examples are used to demonstrate the efficiency and robustness of this method. It is found that the accelerating convergence algorithm proposed in this study proves to be very efficient for it can reduce the iteration number greatly, and LSLIM is also efficient for it can assure the convergence of the iteration of the reliability index.
基金funded by the Horizontal Subject and the National Natural Science Foundation of China(Grant No.81401194).
文摘Testicular endothelial cells have been found to play an important role in spermatogenesis and fertility,but their mechanism is obscure.Exosomes released by various cells are recognized as cell–cell communication mediators during the initiation and progression of many diseases.Therefore,the current study aimed to investigate the protein and miRNA components of human testicular endothelial cell-derived exosomes(HTEC-Exos)and to explore their potential effects on spermatogenesis.In this study,HTEC-Exos were first isolated by the ultracentrifugation method,and then identified by nanoparticle tracking analysis,transmission electron microscopy(TEM),and western blotting.The characteristics of HTEC-Exos were examined by liquid chromatography–mass spectrometry and microRNA(miRNA)chip analysis.Bioinformatics analysis was performed to explore the potential role of the exosomal content on spermatogenesis.A total of 945 proteins were identified,11 of which were closely related to spermatogenesis.A total of 2578 miRNAs were identified.Among them,30 miRNAs demonstrated potential associations with male reproductive disorders,such as azoospermia,and spermatogenesis disorders.In particular,11 out of these 30 miRNAs have been proven to be involved in spermatogenesis based on available evidence.This study provides a global view of the proteins and miRNAs from HTEC-Exos,suggesting that HTEC-Exos may function as potential effectors during the process of spermatogenesis.
基金supported by the Major Program of National Natural Science Foundation of China(No.42090055)the National Natural Science Foundation of China(No.41977242)the Fundamental Research Funds for the Central Universities,China(No.CUGGC09)。
文摘In engineering practice,the stability of a slope is often analyzed as a 2D problem assuming a plane-strain condition,which may lead to significant errors.In this paper,a comprehensive study is carried out to compare the results of 2D and 3D slope stability analyses,using the strength reduction method for deterministic analysis and the random field approach for probabilistic analysis,respectively.The results of this comparison study confirm that in the deterministic stability evaluation,the 2D analysis tends to obtain a smaller factor of safety than does its 3D counterpart.In the probabilistic evaluation that considers the spatial variability of soil properties,the 2D analysis tends to yield a larger probability of failure than its 3D counterpart.A significant feature of the 3D probabilistic slope stability analysis is the presence of multiple local failures distributed along the slope longitudinal direction.This paper provides insights regarding the degree of errors in modeling a 3D slope as a 2D problem,which can be regarded as a complement to the previous 3D slope stability analyses.
