Helicobacter pylori infection, gastrin and cyclooxygenase-2 in gastric carcinogenesis

Gastric cancer is one of the most frequent neoplasms and a main cause of death worldwide, especially in China and Japan. Numerous epidemiological, animal and experimental studies support a positive association between chronic Helicobacter pylori(H. pylori) infection and the development of gastric cancer. However, the exact mechanism whereby H. pylori causes gastric carcinogenesis remains unclear. It has been demonstrated that expression of cyclooxygenase-2(COX-2) is elevated in gastric carcinomas and in their precursor lesions. In this review, we present the latest clinical and experimental evidence showing the role of gastrin and COX-2 in H. pylori-infected patients and their possible association with gastric cancer risk.

Gastric cancer cell lines induced by trichostatin A

AIM: To explore the effect of trichostatin A (TSA) on apoptosis and acetylated histone H3 levels in gastric cancer cell lines BGC-823 and SGC-7901. METHODS: The effect of TSA on growth inhibition and apoptosis was examined by MTT, fluorescence microscopy and PI single-labeled flow cytometry. The acetylated histone H3 level was detected by Western blot. RESULTS: TSA induced apoptosis in gastric cancer cell lines BGC-823 and SGC-7901 was in a dose and time-dependent manner. Apoptotic cells varied significantly between TSA treated groups (37.5 ng/mL 72 h for BGC-823 cell line and 75 ng/mL 72 h for SGC-7901 cell line) and control group (0.85 ± 0.14 vs 1.14 ± 0.07, P = 0.02; 0.94 ± 0.07 vs 1.15 ± 0.06, P = 0.02). Morphologic changes of apoptosis, including nuclear chromatin condensation and fluorescence strength, were observed under fluorescence microscopy. TSA treatment in BGC-823 and SGC-7901 cell lines obviously induced cell apoptosis, which was demonstrated by the increased percentage of sub-G1 phase cells, the reduction of G1-phase cells and the increase of apoptosis rates in flow cytometric analysis. The result of Western blot showed that the expression of acetylated histone H3 increased in BGC-823 and SGC-7901 TSA treatment groups as compared with the control group.CONCLUSION: TSA can induce cell apoptosis in BGC-823 and SGC-7901 cell lines. The expression of acetylated histone H3 might be correlated with apoptosis.

Effects of Pitavastatin on Lipoprotein Subfractions and Oxidized Low-density Lipoprotein in Patients with Atherosclerosis

It has been demonstrated that pitavastatin can significantly reduce low-density lipoprotein(LDL)cholesterol(LDL-C),but its impact on lipoprotein subfractions and oxidized low-density lipoprotein(oxLDL)has not been determined.The aim of the present study was to investigate the potential effects of pitavastatin on subfractions of LDL and high-density lipoprotein(HDL)as well as oxLDL in untreated patients with coronary atherosclerosis(AS).Thirty-six subjects were enrolled in this study.O f them,18 patients with AS were administered pitavastatin 2 mg/day for 8 weeks and 18 healthy subjects without therapy served as controls.The plasma lipid profile,lipoprotein subfractions and circulating oxLDL were determined at baseline and 8 weeks respectively.The results showed that pitavastatin treatment indeed not only decreased LDL-C,total cholesterol(TC),triglycerides(TG)and apolipoprotein B(ApoB)levels,and increased HDL cholesterol(HDL-C),but also reduced the cholesterol concentration of all of the LDL subfractions and the percentage of intermediate and small LDL subfractions.Meanwhile,pitavastatin could decrease plasma oxLDL levels.Furthermore,a more close correlation was found between oxLDL and LDL-C as well as LDL subfractions after pitavastatin treatment.We concluded that a moderate dose of pitavastatin therapy not only decreases LDL-C and oxLDL concentrations but also improves LDL subfractions in patients with AS.

Clinical features of coronary artery ectasia in the elderly

ObjectiveTo 与冠的动脉 ectasia (CAE ) 在老病人调查发生，成像和临床的特征 .MethodsA 回顾的分析与 CAE 在病人上被进行经历了在 2006 年 1 月和 2012 年 12 月之间的冠的 angiography。根据年龄，注册病人被划分成二个组(老组，年龄 ≥；65 年；非老的组，年龄 <；65 年) 。临床的特征，成像特征和二个组的 5 年的幸存率是在老病人的 CAE 的 compared.ResultsThe 流行是 0.33% 。在老组的病人被发现有女性的显著地更高的比例(30.1% 对 10.1% ， P <；0.001 ) ，三容器的疾病(60.5% 对 45.2% ， P = 0.003 ) 并且局部性的 ectasia (55.0% 对 40.2% ， P = 0.003 ) 。另外，身体团索引(20.90 ±；2.71 kg/m 2 对 22.31 ±；2.98 kg/m 2, P <；0.001 ) 并且当前的吸烟者的百分比(45.0% 对 64.6% ， P <；0.001 ) 在老组是显著地更低的。累积幸存曲线与非老的组相比在老组在后续表明了减少的 5 年的累积幸存(88.0% 对 96.0% ， P = 0.002 ) 。但是免费幸存率没能显示出二之间的重要差别的 5 年的事件组织(31.0% 对 35.0% ， P = 0.311 ) 在老病人的 CAE 的 .ConclusionThe 流行是 0.33% ，它关于 CAE 病人的全部数字的 1/3。在之间有重要差别老并且有 CAE 的非老的病人以冠的动脉疾病冒险因素和冠的动脉 ectatic 特征。CAE 可能与增加的死亡风险被联系在老。

High-fat diet aggravates colitis via mesenteric adipose tissue derived exosome metastasis-associated lung adenocarcinoma transcript 1

BACKGROUND Obesity is associated with an increased risk of developing Crohn’s disease(CD),higher disease activity,and comparatively worse clinical outcomes.AIM To investigate the role of mesenteric adipose tissue-derived exosomes in the pathogenesis of CD aggravation in obese individuals.METHODS First,we induced colitis in mice initiated on high-fat and normal diets and compared the severity of colitis.We then extracted and identified exosomes from mesenteric adipose tissue and determined the levels of metastasis-associated lung adenocarcinoma transcript 1(MALAT1)in mesenteric adipose tissue-derived exosomes and the colon.Next,we demonstrated an interaction between MALAT1 and the miR-15a-5p/activating transcription factor 6(ATF6)axis.Finally,we explored the effects of mesenteric adipose tissue-derived exosomes extracted from mice fed a high-fat or normal diet on the severity of 2,4,6-trinitrobe-nzenesulfonic acid(TNBS)-induced colitis and ATF6-related endoplasmic reticulum stress pathways.RESULTS High-fat diet was found to aggravate TNBS-induced colitis in mice.The expression of MALAT1 in mesenteric adipose tissue-derived exosomes of high-fat diet-fed mice increased.The increased expression of MALAT1 in colon tissue exacerbated TNBS-induced colitis and activated the ATF6 endoplasmic reticulum stress pathway.This effect was partially reversed by the reduced expression of MALAT1 and overexpression of miR-15a-5p.CONCLUSION Mesenteric adipose tissue-derived exosome-encapsulated long noncoding RNAs MALAT1 targets the colon and aggravates TNBS-induced colitis in obese mice,which may potentially act on the miR-15a-5p/ATF6 axis and activate endoplasmic reticulum stress.

改良传统功法“七字专注功”对注意缺陷多动障碍儿童注意力及感觉统合能力的影响

目的探索改良传统功法“七字专注功”对注意缺陷多动障碍(attention deficit and hyperactive disorder,ADHD)儿童注意力及感觉统合能力的影响。方法选取上海市7~12岁ADHD儿童23例为研究对象,分为试验组(12例)和对照组(11例)。试验组行“七字专注功”练习,对照组行常规认知及注意力训练。以小组课形式开展试验,每节课的患儿控制在3~5人以保证试验效果,每周每位患儿开展1节课,时长60 min,试验共8周。所有患儿均在干预前、干预后、干预结束3周后接受SNAP-IV量表、Conners量表和SPM感觉加工量表评估,并比较评估结果。结果干预后,试验组在过度活跃、品行问题、学习问题、社会参与等方面较对照组有明显改善(P<0.05);视觉、听觉、触觉、躯体知觉、平衡和运动等指数得分均显著高于对照组(P<0.05)。结论“七字专注功”在改善ADHD儿童注意力及感觉统合能力方面有显著的成效。

内镜黏膜下剥离术治疗早期胃癌术后复发的相关因素分析

目的探讨内镜黏膜下剥离术(ESD)治疗早期胃癌(EGC)的术后复发情况及相关危险因素。方法回顾性分析2016年1月-2019年12月青海省人民医院消化内科实施ESD治疗且病理结果为EGC的258例患者的临床及随访资料,分析影响术后复发的相关因素。结果258例患者术后均获得长期随访,随访期间14例复发,复发率为5.4%,平均复发时间为(10.6±4.3)个月。经单因素和多因素统计分析,病变浸润深度为SM2或以上(OR=5.22,95%CI:1.257~21.648,P=0.023)、病变最大径≥4.0 cm(OR=5.23,95%CI:1.331~20.568,P=0.018)是影响ESD术后复发的独立危险因素。结论ESD治疗EGC临床疗效好,病变的浸润深度及大小是影响ESD术后复发的独立危险因素。

Investigation on hydrogen absorption/desorption properties of as-cast La(1-x)Mg_xNi4.25Al0.75(x=0.0, 0.1, 0.2, 0.3) alloys for tritium storage

La_((1-x))Mg_xNi_(4.25)Al_(0.75)(x = 0.0, 0.1, 0.2, 0.3)alloys for tritium storage were prepared by a method of electromagnetic induction melting. The crystal structure and hydrogen storage performance of the as-cast alloys were investigated. The results showed that a single phase of La Ni_4Al was in the alloys with x = 0.0 and 0.1 and that LaNi_4Al and second phase of(La,Mg)Ni)_3 and AlNi_3 were in the alloys with x = 0.2 and 0.3. On the other hand, the plateau pressures of P–C isotherms of the alloys were increased with the rise of the x value from 0.2 to 0.3 and the hydrogen storage capacity was obviously degraded simultaneously. It was found that the alloy had faster absorption kinetics as the proportion of Mg increased from 0.1 to 0.3.

An Overview of Personal Credit Scoring: Techniques and Future Work

Personal credit scoring is the application of financial risk forecasting. It becomes an even important task as financial institutions have been experiencing serious competition and challenges. In this paper, the techniques used for credit scoring are summarized and classified and the new method—ensemble learning model is introduced. This article also discusses some problems in current study. It points out that changing the focus from static credit scoring to dynamic behavioral scoring and maximizing revenue by decreasing the Type I and Type II error are two issues in current study. It also suggested that more complex models cannot always been applied to actual situation. Therefore, how to use the assessment models widely and improve the prediction accuracy is the main task for future research.

Free triiodothyronine in relation to coronary severity at different ages： Gensini score assessment in 4206 euthyroid patients

为了学习在正常范围以内的免费 triiodothyronine ( FT3 )是否在不同的性和年龄 groups.MethodsA 在冠的动脉疾病( CAD )的存在和严厉上有效果， 4206 个 euthyroid 病人总计连续地被注册并且把组划分了成 CAD ( n = 3306 )并且 非CAD 组( n = 900 )。所有病人经历了冠的 angiography (CAG ) 。Gensini 分数(GS ) 被用来决定冠的动脉狭窄的严厉。严重 CAD 被定义为 GS > 32 并且温和 CAD 被定义为 GS 32。逻辑回归分析和线性回归分析被进行与不同的性在病人与 CAD 决定 FT3 的协会， FT3 的 ages.ResultsConcentration 在 angiography 正常的控制组比那在有 CAD 的病人是更低的(P < 0.05 ) 。另外， FT3 的集中在温和 CAD 比那在严重 CAD 是更低的。在为传统的心血管的风险因素和潜在的 confounders 调整以后， FT3 否定地与 CAD 的存在被相关，然而并非在老病人(> 65 岁) 。Multivariable 线性回归分析证明 FT3 否定地在正常以内与稳定的 CAD 在男、年轻的病人，然而并非在旧 patients.ConclusionsLow FT3 与 GS 被联系范围否定地在年轻病人，然而并非在旧的与 CAD 的存在和严厉被联系。进一步的研究被需要证实我们的调查结果。

Manipulating transition of a two-component Bose–Einstein condensate with a weak δ-shaped laser

We theoretically study the transition dynamics of a two-component Bose-Einstein condensate driven by a train of weak δ-shaped laser pulses. We find that the atomic system can experience peculiar resonant transition even under weak optical excitations and derive the resonance condition by the perturbation method. Employing this mechanism, we propose a scheme to obtain an atomic ensemble with desired odd/even atom number and also a scheme to prepare a nonclassical state of the many-body system with fixed atom number.

Beam-Switching Antenna Based on Plane Dipole Structure

A novel method for designing a beam-switching antenna with the plane dipole is presented. The antenna is composed of double dipoles placed at the center of an active square structure that is divided in four equal sectors by metallic sheets. Metallic patches at the outside of the structure are used to enhance the radiation performance of the antenna. In each step, the diodes in one sector are on, whereas other diodes are off. The sector with off-state diodes defines the direction of the radiation pattern. An antenna model is designed on the substrate of FR4. The proposed antenna operates from 4.8 GHz to 5.5 GHz with gain of 6.3 dBi and F/B （front to back ratio） of 13.2 dBi when the operating frequency is 5.2 GHz. The antenna radiation pattern can be swept in the entire azimuth plane in four steps with a 3 dB beamwidth of 90%. The results reveal that the antenna could be used in the base station of the wireless communication systems.

A novel mutation in PRPF31,causative of autosomal dominant retinitis pigmentosa,using the BGISEQ-500 sequencer

AIM： To study the genes responsible for retinitis pigmentosa.METHODS： A total of 15 Chinese families with retinitis pigmentosa, containing 94 sporadically afflicted cases, were recruited. The targeted sequences were captured using the Target_Eye_365_V3 chip and sequenced using the BGISEQ-500 sequencer, according to the manufacturer＇s instructions. Data were aligned to UCSC Genome Browser build hg19, using the Burroughs Wheeler Aligner MEM algorithm. Local realignment was performed with the Genome Analysis Toolkit（GATK v.3.3.0） Indel Realigner, and variants were called with the Genome Analysis Toolkit Haplotypecaller, without any use of imputation. Variants were filtered against a panel derived from 1000 Genomes Project, 1000 G_ASN, ESP6500, Ex AC and db SNP138. In all members of Family ONE and Family TWO with available DNA samples, the genetic variant was validated using Sanger sequencing.RESULTS： A novel, pathogenic variant of retinitis pigmentosa, c.357_358 del AA（p.Ser119 Serfs X5） was identified in PRPF31 in 2 of 15 autosomal-dominant retinitis pigmentosa（ADRP） families, as well as in one, sporadic case. Sanger sequencing was performed uponprobands, as well as upon other family members. This novel, pathogenic genotype co-segregated with retinitis pigmentosa phenotype in these two families. CONCLUSION： ADRP is a subtype of retinitis pigmentosa, defined by its genotype, which accounts for 20%-40% of the retinitis pigmentosa patients. Our study thus expands the spectrum of PRPF31 mutations known to occur in ADRP, and provides further demonstration of the applicability of the BGISEQ500 sequencer for genomics research.

Mass spectrometry imaging and single-cell transcriptional profiling reveal the tissue-specific regulation of bioactive ingredient biosynthesis in Taxus leaves

Taxus leaves provide the raw industrial materials for taxol,a natural antineoplastic drug widely used in the treatment of various cancers.However,the precise distribution,biosynthesis,and transcriptional regulation of taxoids and other active components in Taxus leaves remain unknown.Matrix-assisted laser desorption/ionization–mass spectrometry imaging analysis was used to visualize various secondary metabolites in leaf sections of Taxus mairei,confirming the tissue-specific accumulation of different active metabolites.Single-cell sequencing was used to produce expression profiles of 8846 cells,with a median of 2352 genes per cell.Based on a series of cluster-specific markers,cells were grouped into 15 clusters,suggesting a high degree of cell heterogeneity in T.mairei leaves.Our data were used to create the first Taxus leaf metabolic single-cell atlas and to reveal spatial and temporal expression patterns of several secondary metabolic pathways.According to the cell-type annotation,most taxol biosynthesis genes are expressed mainly in leaf mesophyll cells;phenolic acid and flavonoid biosynthesis genes are highly expressed in leaf epidermal cells(including the stomatal complex and guard cells);and terpenoid and steroid biosynthesis genes are expressed specifically in leaf mesophyll cells.A number of novel and cell-specific transcription factors involved in secondary metabolite biosynthesis were identified,including MYB17,WRKY12,WRKY31,ERF13,GT_2,and bHLH46.Our research establishes the transcriptional landscape of major cell types in T.mairei leaves at a single-cell resolution and provides valuable resources for studying the basic principles of cell-type-specific regulation of secondary metabolism.

A deep learning interatomic potential suitable for simulating radiation damage in bulk tungsten

So far, it has been a challenge for existing interatomic potentials to accurately describe a wide range of physical properties and maintain reasonable efficiency. In this work, we develop an interatomic potential for simulating radiation damage in body-centered cubic tungsten by employing deep potential, a neural network-based deep learning model for representing the potential energy surface. The resulting potential predicts a variety of physical properties consistent with first-principles calculations, including phonon spectrum, thermal expansion, generalized stacking fault energies, energetics of free surfaces, point defects, vacancy clusters, and prismatic dislocation loops. Specifically, we investigated the elasticity-related properties of prismatic dislocation loops, i.e., their dipole tensors, relaxation volumes, and elastic interaction energies. This potential is found to predict the maximal elastic interaction energy between two 1/2 <1 1 1> loops better than previous potentials, with a relative error of only 7.6%. The predicted threshold displacement energies are in reasonable agreement with experimental results, with an average of 128 eV. The efficiency of the present potential is also comparable to the tabulated gaussian approximation potentials and modified embedded atom method potentials, meanwhile, can be further accelerated by graphical processing units. Extensive benchmark tests indicate that this potential has a relatively good balance between accuracy, transferability, and efficiency.

A Nomogram for Predicting the Likelihood of Obstructive Sleep Apnea to Reduce the Unnecessary Polysomnography Examinations

Background：The currently available polysomnography （PSG） equipments and operating personnel are facing increasing pressure,such situation may result in the problem that a large number of obstructive sleep apnea （OSA） patients cannot receive timely diagnosis and treatment,we sought to develop a nomogram quantifying the risk of OSA for a better decision of using PSG,based on the clinical syndromes and the demographic and anthropometric characteristics.Methods：The nomogram was constructed through an ordinal logistic regression procedure.Predictive accuracy and performance characteristics were assessed with the area under the curve （AUC） of the receiver operating characteristics and calibration plots,respectively.Decision curve analyses were applied to assess the net benefit of the nomogram.Results：Among the 401 patients,73 （18.2%） were diagnosed and grouped as the none OSA （apnea-hypopnea index [AHI] 〈5）,67 （16.7%） the mild OSA （5 ≤ AHI 〈 15）,82 （20.4%） the moderate OSA （15 ≤ AHI 〈 30）,and 179 （44.6%） the severe OSA （AHI ≥ 30）.The multivariable analysis suggested the significant factors were duration of disease,smoking status,difficulty of falling asleep,lack of energy,and waist circumference.A nomogram was created for the prediction of OSA using these clinical parameters and was internally validated using bootstrapping method.The discrimination accuracies of the nomogram for any OSA,moderate-severe OSA,and severe OSA were 83.8%,79.9%,and 80.5%,respectively,which indicated good calibration.Decision curve analysis showed that using nomogram could reduce the unnecessary polysomnography （PSG） by 10% without increasing the false negatives.Conclusions：The established clinical nomogram provides high accuracy in predicting the individual risk of OSA.This tool may help physicians better make decisions on PSG arrangement for the patients referred to sleep centers.

Factors Related to Complicated Grief among Bereave Individuals after the Wenchuan Earthquake in China

Background： The Wenchuan earthquake in China caused shock and grief worldwide. Sudden bereavement caused by the earthquake led to physical disorders as well as psychological disturbances in the bereaved individuals. The bereaved had a high risk for complicated grief （CG）, which may have led to significant distress and impairment in their health. However, there was few available studies on CG among disaster-bereaved individuals in China after the disaster. The aim of this study was to identify factors （demographic characteristics and disaster-related variables） associated with symptoms of CG among the bereaved 18 months after the Wenchuan earthquake. Methods： This study was conducted with a cross-sectional design and a convenience sample of 271 bereaved individuals from three of the hardest hit areas. Data were collected by questionnaires and the instruments used in the study were： General questionnaire and Inventory of CG （ICG）. Multivariate linear regression analysis was used to identify factors associated with symptoms of CG. Results： The mean score on ICG was 52.77 （standard deviation： 10.00）. Being female and loss of a child were related to higher level of CG while having another child after the disaster and receiving psychological counseling experience were associated with lower level of CG. Forty-nine percent of the variance of CG was explained by these identified factors. Conclusions： Eighteen months after the Wenchuan earthquake, the symptoms of CG among the bereaved were higher than the previous studies with bereaved individuals. This study uncovers a vulnerable population of the bereaved at high risk for CG. Early assessments, targeted interventions, and policy support tailored for the disaster-bereaved individuals are necessary to identify and alleviate symptoms of CG and to improve their well-being.

Coordinated Workload Scheduling in Hierarchical Sensor Networks for Data Fusion Applications

To minimize the execution time of a sensing task over a multi-hop hierarchical sensor network, we present a coordinated scheduling method following the divisible load scheduling paradigm. The proposed scheduling strategy builds on eliminating transmission collisions and idle gaps between two successive data transmissions. We consider a sensor network consisting of several clusters. In a cluster, after related raw data measured by source nodes are collected at the fusion node, in-network data aggregation is further considered. The scheduling strategies consist of two phases： intra-cluster scheduling and inter-cluster scheduling. Intra-cluster scheduling deals with assigning different fractions of a sensing workload among source nodes in each cluster; inter-cluster scheduling involves the distribution of fused data among all fusion nodes. Closed-form solutions to the problem of task scheduling are derived. Finally, numerical examples are presented to demonstrate the impacts of different system parameters such as the number of sensor nodes, measurement, communication, and processing speed, on the finish time and energy consumption.

Efficient vulnerability detection based on an optimized rule-checking static analysis technique

Static analysis is an efficient approach for software assurance. It is indicated that its most effective usage is to perform analysis in an interactive way through the software development process, which has a high performance requirement. This paper concentrates on rule-based static analysis tools and proposes an optimized rule-checking algorithm. Our technique improves the performance of static analysis tools by filtering vulnerability rules in terms of characteristic objects before checking source files. Since a source file always contains vulnerabilities of a small part of rules rather than all, our approach may achieve better performance. To investigate our technique's feasibility and effectiveness, we implemented it in an open source static analysis tool called PMD and used it to conduct experiments. Experimental results show that our approach can obtain an average performance promotion of 28.7% compared with the original PMD. While our approach is effective and precise in detecting vulnerabilities, there is no side effect.

Influence of cooling rate on phase transformation and precipitation behavior of Ti-bearing steel in continuous cooling process

The influence of cooling rate on microstructural evolution and precipitation behavior in Ti,Ti–Nb and Ti-Mo low-carbon steels during the continuous cooling process was studied by dilatometer method,optical microscopy,and transmission electron microscopy.The results indicated that austenite transformation temperature decreased with the increasing cooling rate in three steels.The addition of Nb and Mo promoted bainite and martensite transformation and improved the hardenability of steels.In addition,precipitates formed in deformed austenite and ferrite can be observed simultaneously.Deformation in the austenite non-recrystallization zone can introduce a large number of deformation bands,and then,the precipitates preferentially nucleated in these deformation bands.In the following process,randomly distributed precipitates and interphase precipitates will be formed in ferrite.The precipitates formed in deformed austenite obey Kurdjumov-Sachs orientation relationship with the matrix,while the precipitates formed in ferrite obey Baker-Nutting orientation relationship with the matrix.The addition of Nb and Mo in Ti-bearing steels decreased the precipitates size and increased the number density of precipitates and then improved the precipitation hardening.And the effect of Mo addition is more obvious than that of Nb addition.