Effect of two surgical approaches on the lung function and prognosis of patients with combined esophagogastric cancer

BACKGROUND Adenocarcinoma of the esophagogastric junction has a center of origin within 5 cm of the esophagogastric junction.Surgical resection remains the main treatment.A transthoracic approach is recommended for Siewert I adenocarcinoma of the esophagogastric junction and a transabdominal approach is recommended for Siewert III adenocarcinoma of the esophagogastric junction.However,there is a need to determine the optimal surgical approach for Siewert II adenocarcinoma of the esophagogastric junction to improve lung function and the prognosis of patients.AIM To investigate and compare the surgical effects,postoperative changes in pulmonary function,and prognoses of two approaches to treating combined esophagogastric cancer.METHODS One hundred and thirty-eight patients with combined esophagogastric cancer treated by general and thoracic surgeries in our hospital were selected.They were divided into group A comprising 70 patients(transabdominal approach)and group B comprising 68 patients(transthoracic approach)based on the surgical approach.The indexes related to surgical trauma,number of removed lymph nodes,indexes of lung function before and after surgery,survival rate,and survival duration of the two groups were compared 3 years after surgery.RESULTS The duration of surgery,length of hospital stay,and postoperative drainage duration of the patients in group A were shorter than those of the patients in group B,and the volume of blood loss caused by surgery was lower for group A than for group B(P<0.05).At the one-month postoperative review,the first second,maximum ventilation volume,forceful lung volume,and lung volume values were higher for group A than for group B(P<0.05).Preoperatively,the QLQ-OES18 scale scores of the patients in group A were higher than those in group B on re-evaluation at 3 mo postoperatively(P<0.05).The surgical complication rate of the patients in group A was 10.00%,which was lower than that of patients in group B,which was 23.53%(P<0.05).CONCLUSION Transabdominal and transthoracic surgical approaches are comparable in treating combined esophagogastric cancer;however,the former results in lesser surgical trauma,milder changes in pulmonary function,and fewer complications.

LINC00511 promotes gastric cancer cell growth by acting as a ceRNA

BACKGROUND Gastric cancer(GC)is one of the most aggressive malignancies,with a high incidence and poor prognosis worldwide.Recently,accumulating evidence has illustrated that long noncoding RNAs(lnc RNAs)play pivotal roles in many cancers.It has been reported that LINC00511 contributes to tumorigenesis in various diseases.However,the role of LINC00511 in GC cell growth remains mostly unknown.AIM To determine whether the lnc RNA LINC00511 exerted its carcinogenic function in GC via the miR-124-3 p/PDK4 axis.METHODS Cell culture and transfection,RNA extraction and quantitative real-time PCR,CCK-8 assay,Colony formation assay,Luciferase reporter assay,RIP assay,RNA pull-down assay,and Western blot analysis were used to show expression and mechanisms of LINC00511 in GC progression and apoptosis.Rescue assays were performed to verify the relationships among LINC00511,miR-124-3 p and PDK4 further.RESULTS The expression of LINC00511 was remarkably upregulated in GC cells compared to that in corresponding normal cell lines.Compared to the controls,cell proliferation was inhibited,and cell apoptosis was increased upon LINC00511knockdown,demonstrating that LINC00511 influenced GC cell growth.An exploration of the molecular mechanism revealed that LINC00511 functioned as a molecular sponge of miR-124-3 p and that PDK4 was a downstream target of miR-124-3 p in GC.Rescue assays showed that the overexpression of PDK4 could partly restore the inhibitory function of si-LINC00511 in GC.CONCLUSION These data demonstrate that LINC00511 promotes gastric cancer cell growth by acting as a ce RNA to regulate the miR-124-3 p/PDK4 axis,which may be a promising therapeutic target for GC.

Expression of annexin A5 in serum and tumor tissue of patients with colon cancer and its clinical significance

AIM To investigate the expression of annexin A5 in serum and tumor tissue of patients with colon cancer and to analyze its clinical significance.METHODS Ninety-three patients with colon cancer treated at our hospital between February 2013 and March 2016 were included in an observation group, and 40 healthy individuals were included in a control group. Enzyme-linked immunosorbent assay was performed to determine the serum level of annexin A5, while immunohistochemistry was performed to determine the expression of annexin A5 in cancer tissues.RESULTS The serum level of annexin A5 was 0.184 ± 0.043 ng/m L in the observation group, which was significantly higher than that in the control group(P < 0.05). Annexin A5 expression was detected in 79.31% of the patients with lymph node metastasis, which was significantly higher than that in patients without lymph node metastasis(P < 0.05). Moreover, annexin A5 expression was detected in 86.96% of the patients with stage Ⅲ to Ⅳ disease, which was significantly higher than that in patients with stage Ⅰ to Ⅱ disease(P < 0.05). The serum level of annexin A5 was 0.215 ± 0.044 ng/m L in patients whose tumors were positive for annexin A5 expression, which was significantly higher than that in patients whose tumors were negative for annexin A5 expression(P < 0.05). The serum level of annexin A5 was correlated with annexin A5 expression in colon cancer tissues(r= 0.312, P < 0.05). When a cutoff value of > 0.148 ng/m L for serum level of annexin A5 was used in the diagnosis of colon cancer, the sensitivity was 83.90%, and the specificity was 57.50%.CONCLUSION For patients with colon cancer, annexin A5 expression in cancer tissues is related to lymph node metastasis and tumor grade. Serum level of annexin A5 is related to annexin A5 expression in cancer tissues and is of diagnostic relevance.

Enantioselective Intramolecular Desymmetric ct-Addition of Cyclohexa- none to Propiolamide Catalyzed by Sodium L-Prolinate

Summary of main observation and conclusion An enantioselective desymmetric nucleophilic α-addition of cyclohexanone to propiolamide has been developed through a 6-exo-dig cyclization reaction.By employing simple and readily available L-proline sodium salt as a bifunctional catalyst,a series of chiral 6,6-bicyclic bridged products bearing morphan scaffold have been isolated in good yields and excellent enantioselectivities.Density functional theory (DFT)calculations elucidated the origins of the enantioselectivity and regioselectivity of this transformation.A salt bridge that links the amide carbonyl group with proline carboxylate in the transition state was proven to be the driving force for the induction of excellent enantioselectivity.