Multiple myeloma(MM)is a malignant neoplasm characterized by clonal proliferation of abnormal plasma cells.In many countries,it ranks as the second most prevalent malignant neoplasm of the hematopoietic system.Althoug...Multiple myeloma(MM)is a malignant neoplasm characterized by clonal proliferation of abnormal plasma cells.In many countries,it ranks as the second most prevalent malignant neoplasm of the hematopoietic system.Although treatment methods for MM have been continuously improved and the survival of patients has been dramatically prolonged,MM remains an incurable disease with a high probability of recurrence.As such,there are still many challenges to be addressed.One promising approach is singlecell RNA sequencing(scRNA-seq),which can elucidate the transcriptome heterogeneity of individual cells and reveal previously unknown cell types or states in complex tissues.In this review,we outlined the experimental workflow of scRNA-seq in MM,listed some commonly used scRNA-seq platforms and analytical tools.In addition,with the advent of scRNA-seq,many studies have made new progress in the key molecular mechanisms during MM clonal evolution,cell interactions and molecular regulation in the microenvironment,and drug resistance mechanisms in target therapy.We summarized the main findings and sequencing platforms for applying scRNA-seq to MM research and proposed broad directions for targeted therapies based on these findings.展开更多
Objective:To observe the clinical therapeutic effects on nervous olfactory disorder treated with acupuncture at three nasal points and scalp acupuncture.Methods:Acupuncture was applied in 30 eligible patients with ner...Objective:To observe the clinical therapeutic effects on nervous olfactory disorder treated with acupuncture at three nasal points and scalp acupuncture.Methods:Acupuncture was applied in 30 eligible patients with nervous olfactory disorder.The points included three nasal points,i.e.,Yíngxiāng(迎香 LI 20),Shàngyíngxiāng(上迎香 EX-HN 8)and Yìntáng(印堂EX-HN 3),ézhōngxiàn(额中线 middle line of forehead,MS1)and bilateral Nièhòuxiàn(颖后线 posterior temporal line,MS11).The even-needling technique was used to stimulate the three nasal points to ensure deqi. In scalp acupuncture,the needle was rotated continuously at 200 times per min,for 2 min at each scalp acupuncture line.The needles were retained for 40 min at above points and lines and were manipulated once every 20 min during needle retaining.The treatment was given once a day,6-day treatment was as one course and the next course of treatment started after the interval of 1 day.Totally,4 courses of treatment were required.Before and after treatment,T&T olfactory was adopted to test the olfactory function and the score was compared in order to evaluate the therapeutic effect.Results:Of 30 cases,8 cases were cured,20 cases remarkably effective and 2 cases failed.The total effective rate was 93%.Conclusion:Acupuncture at three nasal points and scalp acupuncture are effective approach to nervous olfactory disorder.展开更多
基金supported by the Shanghai Shenkang Hospital Development Center(No.SHDC2020CR2070B).
文摘Multiple myeloma(MM)is a malignant neoplasm characterized by clonal proliferation of abnormal plasma cells.In many countries,it ranks as the second most prevalent malignant neoplasm of the hematopoietic system.Although treatment methods for MM have been continuously improved and the survival of patients has been dramatically prolonged,MM remains an incurable disease with a high probability of recurrence.As such,there are still many challenges to be addressed.One promising approach is singlecell RNA sequencing(scRNA-seq),which can elucidate the transcriptome heterogeneity of individual cells and reveal previously unknown cell types or states in complex tissues.In this review,we outlined the experimental workflow of scRNA-seq in MM,listed some commonly used scRNA-seq platforms and analytical tools.In addition,with the advent of scRNA-seq,many studies have made new progress in the key molecular mechanisms during MM clonal evolution,cell interactions and molecular regulation in the microenvironment,and drug resistance mechanisms in target therapy.We summarized the main findings and sequencing platforms for applying scRNA-seq to MM research and proposed broad directions for targeted therapies based on these findings.
文摘Objective:To observe the clinical therapeutic effects on nervous olfactory disorder treated with acupuncture at three nasal points and scalp acupuncture.Methods:Acupuncture was applied in 30 eligible patients with nervous olfactory disorder.The points included three nasal points,i.e.,Yíngxiāng(迎香 LI 20),Shàngyíngxiāng(上迎香 EX-HN 8)and Yìntáng(印堂EX-HN 3),ézhōngxiàn(额中线 middle line of forehead,MS1)and bilateral Nièhòuxiàn(颖后线 posterior temporal line,MS11).The even-needling technique was used to stimulate the three nasal points to ensure deqi. In scalp acupuncture,the needle was rotated continuously at 200 times per min,for 2 min at each scalp acupuncture line.The needles were retained for 40 min at above points and lines and were manipulated once every 20 min during needle retaining.The treatment was given once a day,6-day treatment was as one course and the next course of treatment started after the interval of 1 day.Totally,4 courses of treatment were required.Before and after treatment,T&T olfactory was adopted to test the olfactory function and the score was compared in order to evaluate the therapeutic effect.Results:Of 30 cases,8 cases were cured,20 cases remarkably effective and 2 cases failed.The total effective rate was 93%.Conclusion:Acupuncture at three nasal points and scalp acupuncture are effective approach to nervous olfactory disorder.
