不同内固定方案治疗Meyers-Mckeever Ⅲ、Ⅳ型胫骨髁间棘骨折临床对比研究

目的探讨微创关节镜下医用钢丝与Orthocord缝线内固定方案治疗Meyers-Mckeever Ⅲ、Ⅳ型胫骨髁间棘骨折临床疗效及安全性差异。方法选取该院2011年6月-2015年6月收治胫骨远端闭合性骨折患者100例。以随机数字表法分为对照组(50例)和观察组(50例),分别采用微创关节镜下医用钢丝与Orthocord缝线内固定方案治疗。比较两组患者手术时间、止血带应用时间、骨性愈合时间、术后HSS评分、Lysholm评分、膝关节屈伸活动度、Lachman试验阳性率及并发症发生率等。结果观察组患者手术时间与对照组比较,差异有统计学意义(P<0.05),观察组短于对照组;两组患者止血带应用时间和骨性愈合时间比较差异无统计学意义(P>0.05);观察组患者术后HSS评分、Lysholm评分及膝关节屈伸活动度与对照组比较,差异有统计学意义(P<0.05),观察组均优于对照组;两组患者术后Lachman试验阳性率比较差异无统计学意义(P>0.05);两组患者术后并发症发生率比较差异无统计学意义(P>0.05)。结论微创关节镜下Orthocord缝线内固定方案治疗Meyers-MckeeverⅢ、Ⅳ型胫骨髁间棘骨折可有效缩短手术用时,提高术后关节功能恢复效果,且未增加并发症发生概率,价值优于医用钢丝。

RETINOIC ACID DOWN-REGULATES BONE MORPHOGENETIC PROTEIN 7 EXPRESSION IN RAT WITH CLEFT PALATE

Objective To evaluate the effects of retinoic acid(RA)on expression of bone morphogenetic protein 7(BMP-7)in rat fetus with cleft palate,and the effects of RA on proliferation and apoptosis of osteoblasts.Methods All-trans RA(ATRA)was used to induce congenital cleft palate in Wistar rat.BMP-7 mRNA expression in maxillary bone tissue of fetal rats was measured by Northern blotting analysis.Flow cytometry and MTT assay were used to measure the apoptosis and proliferation of ATRA-treated MC-3T3-E1 cells.BMP-7 mRNA and protein expressions in ATRA-treated MC-3T3-E1 cells were detected by RT-PCR and Western blotting analysis.Results ATRA could induce cleft palate of rat fetus.The incidence rate of cleft palate induced by 100 mg/kg ATRA(45.5%)was significantly higher than 50 mg/kg ATRA(12.5%,P<0.05).BMP-7 mRNA expression decreased in maxillary bone tissue of rat fetus with cleft palate.MC-3T3-E1 cells proliferation treated with 1×10-6 mol/LATRA decreased by 60%,the cell apoptosis increased by 2 times.BMP-7 mRNA and protein levels in MC-3T3-E1 cells treated with 1×10-6 mol/L ATRA decreased by 60% and 80%,respectively,compared with ATRA-untreated cells(P<0.05).Conclusions BMP-7 may play an important role in embryonic palate development.RA may possess the ability to down-regulate cell proliferation through regulation of BMP-7 gene expression.

东南景天叶肉细胞原生质体和液泡的分离与纯化技术（英文）

目的:在超积累植物东南景天对镉的区隔化过程中,叶肉细胞等内含大型液泡的薄壁细胞起重要作用。本文旨在建立并优化其叶肉细胞原生质体和液泡的提取和纯化技术,在技术层面上为东南景天的镉区隔化机理研究奠定基础,有助于深入探明其超积累镉的生理与分子机理。创新点:优化了东南景天叶片原生质体的提取和纯化技术,并建立了能较高效率获得膜完整性好、数量多、纯度高的液泡提取方法。方法:主要包括原生质体提取、液泡粗提和液泡纯化。原生质体提取:取东南景天叶片,切成1~2 mm的细条状后浸入经预热过的细胞裂解液中,震荡2 h后过滤,离心清洗后获得原生质体。液泡粗提:采用1-丙磺酸浓度为0.675 mmol/L的原生质体裂解液裂解原生质体,离心后获得粗提的液泡,并加入含0.8 mol/L甘露醇的液泡保护液。液泡纯化:往初提液泡的悬浮液下层加入质量体积比浓度为0.10 g/ml的Ficoll溶液,进行密度梯度离心,获取纯化的液泡。结论:细胞裂解液的预热处理可加速细胞壁降解,裂解时间设置为2 h有利于原生质体的高效提取;通过对原生质体裂解液浓度、细胞保护液浓度和梯度离心等参数的改良,可有效提取叶片细胞原生质体中的液泡。