Objective The aim of this study was to examine the effects of photodynamic therapy(PDT)on the expression of Nav1.7 in spinal dorsal root ganglion(DRG)neurons.Methods The primary DRG neurons from newborn SD rats were c...Objective The aim of this study was to examine the effects of photodynamic therapy(PDT)on the expression of Nav1.7 in spinal dorsal root ganglion(DRG)neurons.Methods The primary DRG neurons from newborn SD rats were cultured.The cells were identified by neuron-specific enolase immunofluorescence staining.DRG neurons were divided into four groups:control group,photosensitizer group,laser group,and PDT group.The cell viability was detected by a cell counting kit-8(CCK8)assay.qRT-PCR and Western blotting were used to determine the mRNA and protein expression levels of Nav1.7 in DRG neurons.Results The purity of the cultured primary DRG neurons was greater than 90%.Compared with the control group,no significant change was found in the cell viability of the photosensitizer group,while the viability in the laser group and the PDT group was significantly reduced.The mRNA and protein expression levels of Nav1.7 were significantly greater in the laser group and the PDT group than in the control group.At the same time,the mRNA and protein expression levels of Nav1.7 were greater in the laser group than in the PDT group.Conclusion Both laser and PDT could upregulate the expression of Nav1.7 in DRG neurons,and the promoting effect might be related to the pain induced by clinical treatment.This study provides a research basis for the use of laser and PDT to treat pain.A better understanding of the relationship between Nav1.7 and PDT can help clinicians better manage PDT-related pain.展开更多
BACKGROUND:The study aims to investigate an optimal indicator for changing the filter during the continuous renal replacement therapy(CRRT)in intensive care unit(ICU)patients with acute kidney injury(AKI).METHODS:Pati...BACKGROUND:The study aims to investigate an optimal indicator for changing the filter during the continuous renal replacement therapy(CRRT)in intensive care unit(ICU)patients with acute kidney injury(AKI).METHODS:Patients with AKI requiring CRRT in an ICU were randomly divided into two groups for crossover trial,i.e.,groups A and B.Patients in the group A were firstly treated with continuous veno-venous hemofiltration(CVVH),followed by continuous veno-venous hemodiafiltration(CVVHDF).Patients in the group B were firstly treated with CVVHDF followed by CVVH.Delivered doses of solutes with different molecular weights at the indicated time points between groups were compared.A correlation analysis between the delivered dose and pre-filter pressure(P_(PRE))and transmembrane pressure(P_(TM))was performed.Receiver operating characteristic(ROC)curves were constructed to evaluate the accuracy of P_(TM) as an indicator for filter replacement.RESULTS:A total of 50 cases were analyzed,27 in the group A and 23 in the group B.Delivered doses of different molecular-weight solutes significantly decreased before changing the filter in both modalities,compared with those at the initiation of treatment(all P<0.05).In the late stage of CRRT,the possible rebound of serum medium-molecular-weight solute concentration was observed.P_(TM) was negatively correlated with the delivered dose of medium-molecular-weight solute in both modalities.The threshold for predicting the rebound of serum concentration of medium-molecularweight solute by P_(TM) was 146.5 mm Hg(1 mm Hg=0.133 k Pa).CONCLUSIONS:The filter can be used as long as possible within the manufacturer’s safe use time limits to remove small-molecular-weight solutes.P_(TM) of 146.5 mm Hg may be an optimal indicator for changing the filter in CRRT therapies to remove medium-molecular-weight solutes.展开更多
Objective To investigate the difference in interictal perfusion patterns between refractory and non-refractory temporal lobe epilepsies evaluated with flow-sensitive alternating inversion recovery (FAIR) magnetic reso...Objective To investigate the difference in interictal perfusion patterns between refractory and non-refractory temporal lobe epilepsies evaluated with flow-sensitive alternating inversion recovery (FAIR) magnetic resonance (MR) sequence. Methods Nine patients with refractory temporal lobe epilepsy, 21 patients with non-refractory temporal lobe epilepsy, and 13 normal volunteers underwent FAIR MR scanning. The relative cerebral blood flow (rCBF) in bilateral hemispheres and mesial temporal lobes were measured. And we also calculated the asymmetry index (AI) values. Results The AI values of bilateral hemispheres in refractory and non-refractory epilepsy patients were both significantly different from those of volunteers (P=0.012 and 0.029, respectively). There was significant difference in AI values of bilateral mesial temporal lobes between non-refractory epilepsy patients and volunteers (P=0.049), while no significant difference between refractory epilepsy patients and volunteers. Conclusions The hypoperfusion pattern of interictal refractory temporal lobe epilepsy patients is different from that of non-refractory patients. Although the hypoperfusion tends to extend out of temporal lobes in all patients, the refractory epilepsy patients have a preference of bilateral mesial temporal hypoperfusion, which may be valuable for evaluating prognosis.展开更多
Objective:To investigate the inoculation status of Novel Coronavirus vaccine among college students in Hainan province and its related influencing factors,and to provide scientific basis for improving the inoculation ...Objective:To investigate the inoculation status of Novel Coronavirus vaccine among college students in Hainan province and its related influencing factors,and to provide scientific basis for improving the inoculation rate of this population.Methods:An electronic questionnaire survey was conducted among 7 colleges and universities in Hainan Province from April 16 to 19,2021.A total of 5896 valid questionnaires were collected with informed consent and voluntary filling.The questionnaire collected information including general demographic characteristics,knowledge attitude and behavioral problems related to COVID-19 vaccine.The difference in the vaccination rate of college students with different characteristics was compared.Multivariate Logistic regression analysis was used to screen the related factors affecting the vaccination rate of college students in Hainan.Results:There were 3,360(57%)female students,2,509(42.6%)medical students,2,380(40.4%)Hainan students,3,441(58.4%)undergraduates,3,281(57.3%)rural students,and 5,165(87.6%)Han students.The inoculation rate of COVID-19 vaccine among college students in Hainan province was 81.7%.The average score of knowledge about COVID-19 vaccine was 11.78±3.18,the total score was 17,and the relative attitude score was 47.77±6.10,the total score was 55.Univariate analysis found that difference gender,age,grade,stage of study,medical students,Hainanese origin,school organization vaccination,vaccine knowledge and attitude scores,the difference of inoculation rate was statistically significant(P<0.05).Multivariate analysis showed that women(OR=1.204),medical profession(OR=4.587),native place of Hainan(OR=1.541),rural area of origin(OR=1.311),school carrying out collective vaccine vaccination(OR=2.933),high scores of knowledge about COVID vaccine(OR=1.104)and attitude(OR=1.025)could increase the vaccination rate of college students(P<0.05).Conclusion:The vaccination rate of college students in Hainan Province still needs to be improved.It is necessary to strengthen the popularization of vaccine-related knowledge among male students and non-medical students,improve the mastery of the knowledge of the vaccine and enhance the confidence of the vaccine in China,which is conducive to the improvement of the vaccination rate of college students.展开更多
Objective:To understand the epidemic characters of COVID-19 epidemic in Hainan province of tropical China and provid evidences for the prevention and control of the epidemic.Methods:Data on the COVID-19 epidemic colle...Objective:To understand the epidemic characters of COVID-19 epidemic in Hainan province of tropical China and provid evidences for the prevention and control of the epidemic.Methods:Data on the COVID-19 epidemic collected from Health Commission of Hainan Province were analyzed by using the methods of retrospective descriptive epidemiology.Results:From January 22nd to February 21st,2020,COVID-19 was diagnosed in 168 confirmed cases(including 8 severe cases)in Hainan Province with the temporary fatality rate of 2.38%of 4 deaths,and 96 cases discharged from hospital,with the cure rate of 57.14%.The cases were distributed in altogether 15 cities and counties in Hainan Province,among which 121 cases were distributed in Sanya,Haikou,Danzhou and Wanning,accounting for 72.02%of the total number of cases.Cases in Sanya City were mainly found in Tianya district and Jiyang district,accounting for 69.81%of the total number of cases in Sanya.Cases in Haikou City were mainly found in Qiongshan district and Xiuying district,accounting for 72.5%of the total number of cases in Haikou.Among the patients,there were 81 males and 87 females,with a male-to-female ratio of 1.1;the youngest age was 3 months,the oldest was 79 years old,80%of the patients were in the age group of 30 to 69 years old,and the number of children and adolescents under 20 years old were relatively low.Conclusion:The daily number of new confirmed cases showed a gradual downward trend in Hainan province,and the epidemic situation was in transition from imported to local renewal,mainly in close contact between family members.With the increase of personnel mobility such as the resumption of work by enterprises and the return of migrant workers to the island,there is a risk of agglomeration epidemic.Prevention and control work in urban and rural areas should take a two-pronged approach.Amid preventing imported cases,more proactive measures should be taken to prevent and control the spread of local cases.展开更多
The mechanical anisotropy,structural properties,electronic band structures and thermal properties of C2N2(CH2),Si2N2(SiH2)and Ge2N2(GeH2)are detailed and investigated in this work.The novel silicon nitride phase Si2N2...The mechanical anisotropy,structural properties,electronic band structures and thermal properties of C2N2(CH2),Si2N2(SiH2)and Ge2N2(GeH2)are detailed and investigated in this work.The novel silicon nitride phase Si2N2(SiH2)and germanium nitride phase Ge2N2(GeH2)in the Cmc21 structure are proposed in this work.The novel proposed Si2N2(SiH2)and Ge2N2(GeH2)are both mechanically and dynamically stable.The electronic band calculation of the HSE06 hybrid functional shows that C2N2(CH2),Si2N2(SiH2)and Ge2N2(GeH2)are all wide band gap semiconductor materials,and C2N2(CH2)and Si2N2(SiH2)are direct band gap semiconductor materials,while Ge2N2(GeH2)is a quasi-direct band gap semiconductor material,the band gap of C2N2(CH2),Si2N2(SiH2)and Ge2N2(GeH2)are 5.634 eV,3.013 eV,and2.377 eV,respectively.The three-dimensional and plane distributions of Young’s modulus,shear modulus and Poisson’s ratio of C2N2(CH2),Si2N2(SiH2)and Ge2N2(GeH2)show that these materials have different degrees of mechanical anisotropy.The order of Young’s modulus of Si2N2(SiH2)and Ge2N2(GeH2)in different directions is different from that of C2N2(CH2).When the tensile axis is in a particular direction,the order of the Young’s modulus of Si2N2(SiH2):E[110]<E[120]<E[111]<E[101]<E[010]=E[100]<E[011]<E[001],and the order of the Young’s modulus of Ge2N2(GeH2):E[110]<E[111]<E[101]<E[120]<E[100]<E[010]<E[011]<E[001].展开更多
基金supported by Natural Science Foundation of Beijing Municipality(No.7202200)Central Military Commission Health Care Project(No.20BJZ13)Air Force Equipment Scientific Research Project(No.KJ20201A050226).
文摘Objective The aim of this study was to examine the effects of photodynamic therapy(PDT)on the expression of Nav1.7 in spinal dorsal root ganglion(DRG)neurons.Methods The primary DRG neurons from newborn SD rats were cultured.The cells were identified by neuron-specific enolase immunofluorescence staining.DRG neurons were divided into four groups:control group,photosensitizer group,laser group,and PDT group.The cell viability was detected by a cell counting kit-8(CCK8)assay.qRT-PCR and Western blotting were used to determine the mRNA and protein expression levels of Nav1.7 in DRG neurons.Results The purity of the cultured primary DRG neurons was greater than 90%.Compared with the control group,no significant change was found in the cell viability of the photosensitizer group,while the viability in the laser group and the PDT group was significantly reduced.The mRNA and protein expression levels of Nav1.7 were significantly greater in the laser group and the PDT group than in the control group.At the same time,the mRNA and protein expression levels of Nav1.7 were greater in the laser group than in the PDT group.Conclusion Both laser and PDT could upregulate the expression of Nav1.7 in DRG neurons,and the promoting effect might be related to the pain induced by clinical treatment.This study provides a research basis for the use of laser and PDT to treat pain.A better understanding of the relationship between Nav1.7 and PDT can help clinicians better manage PDT-related pain.
基金supported by Kunshan Science and Technology Special Fund(Social Development Category,KS18040)。
文摘BACKGROUND:The study aims to investigate an optimal indicator for changing the filter during the continuous renal replacement therapy(CRRT)in intensive care unit(ICU)patients with acute kidney injury(AKI).METHODS:Patients with AKI requiring CRRT in an ICU were randomly divided into two groups for crossover trial,i.e.,groups A and B.Patients in the group A were firstly treated with continuous veno-venous hemofiltration(CVVH),followed by continuous veno-venous hemodiafiltration(CVVHDF).Patients in the group B were firstly treated with CVVHDF followed by CVVH.Delivered doses of solutes with different molecular weights at the indicated time points between groups were compared.A correlation analysis between the delivered dose and pre-filter pressure(P_(PRE))and transmembrane pressure(P_(TM))was performed.Receiver operating characteristic(ROC)curves were constructed to evaluate the accuracy of P_(TM) as an indicator for filter replacement.RESULTS:A total of 50 cases were analyzed,27 in the group A and 23 in the group B.Delivered doses of different molecular-weight solutes significantly decreased before changing the filter in both modalities,compared with those at the initiation of treatment(all P<0.05).In the late stage of CRRT,the possible rebound of serum medium-molecular-weight solute concentration was observed.P_(TM) was negatively correlated with the delivered dose of medium-molecular-weight solute in both modalities.The threshold for predicting the rebound of serum concentration of medium-molecularweight solute by P_(TM) was 146.5 mm Hg(1 mm Hg=0.133 k Pa).CONCLUSIONS:The filter can be used as long as possible within the manufacturer’s safe use time limits to remove small-molecular-weight solutes.P_(TM) of 146.5 mm Hg may be an optimal indicator for changing the filter in CRRT therapies to remove medium-molecular-weight solutes.
文摘Objective To investigate the difference in interictal perfusion patterns between refractory and non-refractory temporal lobe epilepsies evaluated with flow-sensitive alternating inversion recovery (FAIR) magnetic resonance (MR) sequence. Methods Nine patients with refractory temporal lobe epilepsy, 21 patients with non-refractory temporal lobe epilepsy, and 13 normal volunteers underwent FAIR MR scanning. The relative cerebral blood flow (rCBF) in bilateral hemispheres and mesial temporal lobes were measured. And we also calculated the asymmetry index (AI) values. Results The AI values of bilateral hemispheres in refractory and non-refractory epilepsy patients were both significantly different from those of volunteers (P=0.012 and 0.029, respectively). There was significant difference in AI values of bilateral mesial temporal lobes between non-refractory epilepsy patients and volunteers (P=0.049), while no significant difference between refractory epilepsy patients and volunteers. Conclusions The hypoperfusion pattern of interictal refractory temporal lobe epilepsy patients is different from that of non-refractory patients. Although the hypoperfusion tends to extend out of temporal lobes in all patients, the refractory epilepsy patients have a preference of bilateral mesial temporal hypoperfusion, which may be valuable for evaluating prognosis.
基金Hainan Province Key Research and Development Project(No.zdyf2020109)。
文摘Objective:To investigate the inoculation status of Novel Coronavirus vaccine among college students in Hainan province and its related influencing factors,and to provide scientific basis for improving the inoculation rate of this population.Methods:An electronic questionnaire survey was conducted among 7 colleges and universities in Hainan Province from April 16 to 19,2021.A total of 5896 valid questionnaires were collected with informed consent and voluntary filling.The questionnaire collected information including general demographic characteristics,knowledge attitude and behavioral problems related to COVID-19 vaccine.The difference in the vaccination rate of college students with different characteristics was compared.Multivariate Logistic regression analysis was used to screen the related factors affecting the vaccination rate of college students in Hainan.Results:There were 3,360(57%)female students,2,509(42.6%)medical students,2,380(40.4%)Hainan students,3,441(58.4%)undergraduates,3,281(57.3%)rural students,and 5,165(87.6%)Han students.The inoculation rate of COVID-19 vaccine among college students in Hainan province was 81.7%.The average score of knowledge about COVID-19 vaccine was 11.78±3.18,the total score was 17,and the relative attitude score was 47.77±6.10,the total score was 55.Univariate analysis found that difference gender,age,grade,stage of study,medical students,Hainanese origin,school organization vaccination,vaccine knowledge and attitude scores,the difference of inoculation rate was statistically significant(P<0.05).Multivariate analysis showed that women(OR=1.204),medical profession(OR=4.587),native place of Hainan(OR=1.541),rural area of origin(OR=1.311),school carrying out collective vaccine vaccination(OR=2.933),high scores of knowledge about COVID vaccine(OR=1.104)and attitude(OR=1.025)could increase the vaccination rate of college students(P<0.05).Conclusion:The vaccination rate of college students in Hainan Province still needs to be improved.It is necessary to strengthen the popularization of vaccine-related knowledge among male students and non-medical students,improve the mastery of the knowledge of the vaccine and enhance the confidence of the vaccine in China,which is conducive to the improvement of the vaccination rate of college students.
文摘Objective:To understand the epidemic characters of COVID-19 epidemic in Hainan province of tropical China and provid evidences for the prevention and control of the epidemic.Methods:Data on the COVID-19 epidemic collected from Health Commission of Hainan Province were analyzed by using the methods of retrospective descriptive epidemiology.Results:From January 22nd to February 21st,2020,COVID-19 was diagnosed in 168 confirmed cases(including 8 severe cases)in Hainan Province with the temporary fatality rate of 2.38%of 4 deaths,and 96 cases discharged from hospital,with the cure rate of 57.14%.The cases were distributed in altogether 15 cities and counties in Hainan Province,among which 121 cases were distributed in Sanya,Haikou,Danzhou and Wanning,accounting for 72.02%of the total number of cases.Cases in Sanya City were mainly found in Tianya district and Jiyang district,accounting for 69.81%of the total number of cases in Sanya.Cases in Haikou City were mainly found in Qiongshan district and Xiuying district,accounting for 72.5%of the total number of cases in Haikou.Among the patients,there were 81 males and 87 females,with a male-to-female ratio of 1.1;the youngest age was 3 months,the oldest was 79 years old,80%of the patients were in the age group of 30 to 69 years old,and the number of children and adolescents under 20 years old were relatively low.Conclusion:The daily number of new confirmed cases showed a gradual downward trend in Hainan province,and the epidemic situation was in transition from imported to local renewal,mainly in close contact between family members.With the increase of personnel mobility such as the resumption of work by enterprises and the return of migrant workers to the island,there is a risk of agglomeration epidemic.Prevention and control work in urban and rural areas should take a two-pronged approach.Amid preventing imported cases,more proactive measures should be taken to prevent and control the spread of local cases.
基金Project supported by the National Natural Science Foundation of China(grant numbers 61864004 and 61564005)。
文摘The mechanical anisotropy,structural properties,electronic band structures and thermal properties of C2N2(CH2),Si2N2(SiH2)and Ge2N2(GeH2)are detailed and investigated in this work.The novel silicon nitride phase Si2N2(SiH2)and germanium nitride phase Ge2N2(GeH2)in the Cmc21 structure are proposed in this work.The novel proposed Si2N2(SiH2)and Ge2N2(GeH2)are both mechanically and dynamically stable.The electronic band calculation of the HSE06 hybrid functional shows that C2N2(CH2),Si2N2(SiH2)and Ge2N2(GeH2)are all wide band gap semiconductor materials,and C2N2(CH2)and Si2N2(SiH2)are direct band gap semiconductor materials,while Ge2N2(GeH2)is a quasi-direct band gap semiconductor material,the band gap of C2N2(CH2),Si2N2(SiH2)and Ge2N2(GeH2)are 5.634 eV,3.013 eV,and2.377 eV,respectively.The three-dimensional and plane distributions of Young’s modulus,shear modulus and Poisson’s ratio of C2N2(CH2),Si2N2(SiH2)and Ge2N2(GeH2)show that these materials have different degrees of mechanical anisotropy.The order of Young’s modulus of Si2N2(SiH2)and Ge2N2(GeH2)in different directions is different from that of C2N2(CH2).When the tensile axis is in a particular direction,the order of the Young’s modulus of Si2N2(SiH2):E[110]<E[120]<E[111]<E[101]<E[010]=E[100]<E[011]<E[001],and the order of the Young’s modulus of Ge2N2(GeH2):E[110]<E[111]<E[101]<E[120]<E[100]<E[010]<E[011]<E[001].