Comparative proteomics reveals the response and adaptation mechanisms of white Hypsizygus marmoreus against the biological stress caused by Penicillium

White Hypsizygus marmoreus is a popular edible mushroom.Its mycelium is easy to be contaminated by Penicillium,which leads to a decrease in its quality and yield.Penicillium could compete for limited space and nutrients through rapid growth and produce a variety of harmful gases,such as benzene,aldehydes,phenols,etc.,to inhibit the growth of H.marmoreus mycelium.A series of changes occurred in H.marmoreus proteome after contamination when detected by the label-free tandem mass spectrometry(MS/MS)technique.Some proteins with up-regulated expression worked together to participate in some processes,such as the non-toxic transformation of harmful gases,glutathione metabolism,histone modification,nucleotide excision repair,clearing misfolded proteins,and synthesizing glutamine,which were mainly used in response to biological stress.The proteins with down-regulated expression are mainly related to the processes of ribosome function,protein processing,spliceosome,carbon metabolism,glycolysis,and gluconeogenesis.The reduction in the function of these proteins affected the production of the cell components,which might be an adjustment to adapt to growth retardation.This study further enhanced the understanding of the biological stress response and the growth restriction adaptation mechanisms in edible fungi.It also provided a theoretical basis for protein function exploration and edible mushroom food safety research.

Headspace Solid-Phase Micro-Extraction Gas Chromatography/Mass Spectrometry(HS-SPME-GC/MS)-Based Untargeted Metabolomics Analysis for Comparing the Volatile Components from 12 Panax Herbal Medicines

Quality control of ginseng currently is mainly based on ginsenoside analysis,but rarely focuses on the volatile organic components.In the current work,an untargeted metabolomics approach,by headspace solid-phase micro-extraction gas chromatography/mass spectrometry(HS-SPME-GC/MS),was elaborated and further employed to holistically compare the compositional difference of the volatile components simultaneously from 12 Panax herbal medicines,which included P.ginseng(PG),P.quinquefolius(PQ),P.notoginseng(PN),red ginseng(PGR),P.ginseng leaf(PGL),P.quinquefolius leaf(PQL),P.notoginseng leaf(PNL),P.ginseng flower(PGF),P.quinquefolius flower(PQF),P.notoginseng flower(PNF),P.japonicus(PJ),and P.japonicus var.major(PJvm).Chromatographic separation was performed on an HP-5MS elastic quartz capillary column using helium as the carrier gas,enabling good resolution within 1 h.We were able to characterize totally 259 volatile compounds,including 82 terpenes(T),46 alcohols(Alc),29 ketones(K),25 aldehydes(Ald),21 esters(E),and the others.By analyzing 90 batches of ginseng samples based on the untargeted metabolomics workflows,236 differential ions were unveiled,and accordingly 36 differential volatile components were discovered.It is the first report that simultaneously compares the compositional difference of volatile components among 12 Panax herbal medicines,and useful information is provided for the quality control of ginseng aside from the well-known ginsenosides.

循环肿瘤细胞和循环肿瘤内皮细胞对尿路上皮癌预后的预测价值

目的:探究术前外周血中循环肿瘤细胞(circulating tumor cell,CTC)和循环肿瘤内皮细胞(circulating tumor-derived endothelial cell,CTEC)对尿路上皮癌(urothelial carcinoma,UC)患者预后的预测作用。方法:收集2018年5月至2020年1月于承德医学院附属医院诊治的161例患者的临床病理资料,以100例UC为试验组,46例尿路上皮的良性疾病和15例健康患者为对照组。术前采用差相富集联合免疫染色荧光原位杂交技术(SE-iFISH)富集并染色CTC和CTEC。通过受试者工作特征曲线(receiver operating characteristic curve,ROC)确定CTC和CTEC诊断的截断值,分为阳性组和阴性组,分析其与UC患者临床特征的关系。采用Kaplan-Meier法和Cox比例风险回归模型多因素分析进行生存分析。结果:ROC曲线分析显示术前CTC和CTEC的截断值均为0.5。CTC阳性组和CTEC阳性组中位随访时间分别为12.0个月和12.5个月,阴性组分别为20.0个月和16.5个月。Cox比例风险回归多因素分析显示术前CTEC阳性是UC患者预后的独立危险因子。结论:术前患者外周循环血中的CTC和CTEC对UC患者的预后有预测作用,CTEC为影响预后的独立因素。

Use of a modified ureteral access sheath in semi-rigid ureteroscopy to treat large upper ureteral stones is associated with high stone free rates

Objective:To examine differences in outcomes of semi-rigid ureteroscopy(URS)with or without a modified-ureteral-access-sheath(mUAS)to treat large upper ureteral stones.Methods:Patients with single,radio-opaque large upper ureteral stone(≥10 mm)treated using semi-rigid URS between August 2013 and October 2016 were retrospectively evaluated.The stone-free status was determined from Kidney-ureter-bladder(KUB)X-ray films taken on postoperative Day 1 and after 1 month.Results:Of 103 patients meeting inclusion criteria,43(41.75%)and 60(58.25%)were treated with semi-rigid URS with and without mUAS,respectively.The immediate stone-free rate(SFR)for the mUAS group was significantly higher than the non-mUAS group(40[93.0%]vs.46[76.7%];p=0.033).The SFR at 1 month was also high for patients treated using mUAS,but not statistically different from patients not treated with mUAS(41[95.3%]mUAS vs.51[85.0%]non-mUAS;p=0.115).Auxiliary procedure rates were significantly lower for mUAS patients compared to non-mUAS patients(2[4.7%]vs.14[23.3%];p=0.01).There were no significant differences in surgical duration and hospital stays,and the overall complication rates were statistically similar for mUAS patients compared to non-mUAS patients(1[2.3%]vs.3[5.0%];p=0.638).

循环肿瘤细胞核型对非肌层浸润性膀胱癌预后的预测价值

目的:探究外周血中循环肿瘤细胞(circulating tumor cell,CTC)和循环肿瘤细胞核型对非肌层浸润性膀胱癌(non-muscle invasive bladder cancer,NMIBC)患者预后的预测作用。方法:选取2018年5月至2019年5月于承德医学院附属医院首次诊治的109例患者临床资料,其中NMIBC组58例、良性病变组51例。采用差相富集和免疫荧光染色-染色体荧光原位杂交(SE-iFISH)技术行CTC鉴定及核型分析,以患者CTC三倍体数目≥60%为三倍体组,数目<60%为非三倍体组,并行生存和预后分析。结果:NMIBC组患者的CTC阳性率为81.0%(47/58),显著高于良性病变组的9.8%(5/51)。Kaplan-Meier法和Log-rank法分析显示,术前、术后三倍体组患者的无病生存(disease-free survival,DFS)期明显短于非三倍体组(P<0.001),术后CTC阳性患者的DFS短于CTC阴性患者(P<0.05)。行Cox比例风险回归模型多因素分析显示术前、术后CTC核型是影响NMIBC患者预后的独立危险因素。结论:术前、术后监测CTC和CTC核型对NMIBC患者具有预测作用,CTC核型是影响预后的独立因素。

CPFD modeling of hydrodynamics,combustion and NOx emissions in an industrial CFB boiler

The ultra-low NOx emission requirement(50 mg/m^(3))brings great challenge to CFB boilers in China.To further tap the NOx abatement potential,full understanding the fundamentals behind CFB boilers is needed.To achieve this,a comprehensive CPFD model is established and verified;gas-solid flow,combustion,and NOx emission behavior in an industrial CFB boiler are elaborated;influences of primary air volume and coal particle size on furnace performance are evaluated.Simulation results indicate that there exists a typical core-annular flow structure in the boiler furnace.Furnace temperature is highest in the bottom dense-phase zone(about 950℃)and decreases gradually along the furnace height.Oxygen-deficient combustion results in high CO concentration and strong reducing atmosphere in the lower furnace.NOx concentration gradually increases in the bottom furnace,reaches maximum at the elevation of secondary air inlet,and then decreases slightly in the upper furnace.Appropriate decreasing the primary air volume and coal particle size would increase the CO concentration and intensify the in-furnace reducing atmosphere,which favors for NOx reduction and low NOx emission from CFB boilers.

Effect of seawater salinity on the synthesis of zeolite from coal fly ash

A novel method for the synthesis of zeolite was developed in this paper. The synthesis was carried out by hydrothermal activation after alkali fusion and coal fly ash （CFA） was used as raw material with seawater of different salinities. Seawater salinity was varied from 32 to 88 for zeolite crystallization during the hydrothermal process. The results show that seawater salinity plays an important role in zeolite synthesis with CFA during hydrothermal treatment. The products were a mixture of NaX zeolite and hydroxysodalite; seawater salinity more strongly affected the crystallization than the type and chemical composition of the zeolites. The yield of CFA transformed into zeolite gradually rose with the increase in salinity, reaching a transformation rate of 48%--62% as the salinity increased from 32 to 88, respectively. The proposed method allows for the efficient disposal of by-products; therefore, the application of seawater in zeolite synthesis presents promising economic and ecological benefits.

Revealing the surface electronic structures of AIGaN deep-ultraviolet multiple quantum wells with lateral polarity domains

We report on the carrier dynamic and electronic structure investigations on AlGaN-based deep-ultraviolet multiple quantum wells (MQWs)with lateral polarity domains.The localized potential maximum is predicted near the domain boundaries by first-principle calculation,suggesting carrier localization and efficient radiative recombination.More importantly,lateral band diagrams of the MQWs are proposed based on electron affinities and valance band levels calculated from ultraviolet(UV)photoelectron spectroscopy.The proposed lateral band diagram is further demonstrated by surface potential distribution collected by Kelvin probe microscopy and the density-of-state calculation of energy bands.This work illustrates that lateral polarity structures are playing essential roles in the electronic properties of II nitride photonic devices and may provide novel perspective in the realization of high-efficiency UV emitters.

Tapering-induced enhancement of light extraction efficiency of nanowire deep ultraviolet LED by theoretical simulations

A nanowire(NW) structure provides an alternative scheme for deep ultraviolet light emitting diodes(DUV-LEDs)that promises high material quality and better light extraction efficiency(LEE). In this report, we investigate the influence of the tapering angle of closely packed Al Ga N NWs, which is found to exist naturally in molecular beam epitaxy(MBE) grown NW structures, on the LEE of NW DUV-LEDs. It is observed that, by having a small tapering angle, the vertical extraction is greatly enhanced for both transverse magnetic(TM) and transverse electric(TE) polarizations. Most notably, the vertical extraction of TM emission increased from 4.8% to 24.3%,which makes the LEE reasonably large to achieve high-performance DUV-LEDs. This is because the breaking of symmetry in the vertical direction changes the propagation of the light significantly to allow more coupling into radiation modes. Finally, we introduce errors to the NW positions to show the advantages of the tapered NW structures can be projected to random closely packed NW arrays. The results obtained in this paper can provide guidelines for designing efficient NW DUV-LEDs.

Carrier behavior in the vicinity of pit defects in GaN characterized by ultraviolet light-assisted Kelvin probe force microscopy

Surface potentials in the vicinity of V-pits(cone bottom) and U-pits(blunt bottom) on epitaxial GaN surface have been systematically studied using ultraviolet(UV) light-assisted Kelvin probe force microscopy(KPFM). The band structure models are established to understand variation of the surface potentials at the pits and planar surface with and without UV light. The photo-generated carrier behavior at the pit defects is studied. According to the surface potential results, it can be deduced that the carrier distributions around the V-and U-pits are uneven. In dark, the electron concentration at the bottom of V-pit(30 n_0) and Upit(15 n_0) are higher than that at planar surface(n_0). Under UV light, for V-pit, the electron concentration at the cone bottom(4.93×10^(11) n_0) is lower than that at the surrounding planar surface(5.68×10^(13) n_0). For U-pit, the electron concentration at the blunt bottom is 1.35×10^(12) n_0, which is lower than that at the surrounding planar surface(6.13×10^(13) n_0). The non-equilibrium electron concentrations at different locations are calculated. Based on the non-equilibrium electron concentration, it can be concluded that the carrier recombination rate at pit defects is higher than that at planar surface.

Synthetic Analogues of Betulinic Acid as Potent Inhibitors of PS1/BACE1 Interaction to Reduce Aβ Generation

The lupane-type triterpenoids are endowed with a wide range of biological activities such as antiviral, anti-inflammatory and anticancer activity. We describe here its potential application in Alzheimer＇s disease （AD） treatment as an inhibitor of PS1/BACE1 interaction. 3-a-Akebonoic acid, which emanated from a high throughput screening （HTS）, was discovered to interfere with P S 1/BACE 1 interaction and reduce amyloid β-protein （Aβ） production. In view of the limited source, we instead used naturally rich betulinic acid （compound 2） as starting material for lead optimization and a focused library of its derivatives was constructed to gain a better understanding of the structure activity relationship （SAR） of triterpenoid-type inhibitor of PS1/BACE1 interaction. Compound 22 was finally chosen as the most potent PS 1/BACE 1 interaction inhibitor, which reduced Aβgeneration effectively.

基于顶空进样气相色谱-质谱联用和非靶标代谢组学分析人参、西洋参和三七经蒸制后挥发性成分的转化

人参是全世界范围内广受欢迎的滋补品,经蒸制后补益效果更佳。先前对人参蒸制的研究主要集中在所含的皂苷,但对其挥发性成分研究较少。本研究建立了顶空进样气相色谱-质谱联用结合非靶标代谢组学方法,对人参、西洋参和三七在蒸制过程中挥发性成分的变化进行整体性表征。通过优化GC-MS色谱分离条件,采用HP-5MS弹性石英毛细管柱在38分钟内实现对人参、西洋参和三七生品及蒸制品(1-10 h)中挥发性成分的表征。从人参、西洋参和三七中分别鉴定出了106、106和105种挥发性成分。多元统计分析可以描述不同蒸制时间介导的人参、西洋参和三七的转化轨迹。最终,通过基于GC-MS的非靶标代谢组学分析,发现人参、西洋参和三七蒸制过程中分别有13、16和14种差异成分。值得注意的是,其中异戊醇、2-正戊基呋喃、正己醛、丁香烯和(-)-马兜铃烯可以区分人参的生品和炮制品;α-蒎烯、2-正戊基呋喃、己酸、2,3-丁二醇和丙酮酸可以区分西洋参生品和炮制品;莎草烯、糠醛、Δ-杜松烯、羟基丙酮和1-戊醇可以区分三七生品和炮制品。本研究所建立的气相色谱-质谱联用方法可以揭示中药中挥发性成分的变化,为进一步探讨中药炮制机理提供一种实用的分析工具。

Wide bandgap semiconductor-based integrated circuits

Wide-bandgap semiconductors exhibit much larger energybandgaps than traditional semiconductors such as silicon,rendering them very promising to be applied in the fields of electronics and optoelectronics.Prominent examples of semiconductors include SiC,GaN,ZnO,and diamond,which exhibitdistinctive characteristics such as elevated mobility and thermalconductivity.These characteristics facilitate the operation of awide range of devices,including energy-efficient bipolar junctiontransistors(BJTs)and metal-oxide-semiconductor field-effecttransistors(MOSFETs),as well as high-frequency high-electronmobility transistors(HEMTs)and optoelectronic components suchas light-emitting diodes(LEDs)and lasers.These semiconductorsare used in building integrated circuits(ICs)to facilitate theoperation of power electronics,computer devices,RF systems,andother optoelectronic advancements.These breakthroughs includevarious applications such as imaging,optical communication,andsensing.Among them,the field of power electronics has witnessedtremendous progress in recent years with the development of widebandgap(WBG)semiconductor devices,which is capable ofswitching large currents and voltages rapidly with low losses.However,it has been proven challenging to integrate these deviceswith silicon complementary metal oxide semiconductor(CMOS)logic circuits required for complex control functions.The monolithic integration of silicon CMOS with WBG devices increases thecomplexity of fabricating monolithically integrated smart integrated circuits(ICs).This review article proposes implementingCMOS logic directly on the WBG platform as a solution.However,achieving the CMOS functionalities with the adoption of WBGmaterials still remains a significant hurdle.This article summarizesthe research progress in the fabrication of integrated circuitsadopting various WBG materials ranging from SiC to diamond,with the goal of building future smart power ICs.

Engineering of multiple bound states in the continuum by latent representation of freeform structures

We demonstrate a neural network capable of designing on-demand multiple symmetry-protected bound states in the continuum(BICs)in freeform structures with predefined symmetry.The latent representation of the freeform structures allows the tuning of the geometry in a differentiable,continuous way.We show the rich band inversion and accidental degeneracy in these freeform structures by interacting with the latent representation directly.Moreover,a high design accuracy is demonstrated for arbitrary control of multiple BIC frequencies by using a photonic property readout network to interpret the latent representation.

Enhanced complete photonic bandgap in a moderate refractive index contrast chalcogenide-air system with connected-annular-rods photonic crystals

Connected-annular-rods photonic crystals(CARPCs) in both triangular and square lattices are proposed to enhance the two-dimensional complete photonic bandgap(CPBG) for chalcogenide material systems with moderate refractive index contrast. For the typical chalcogenide-glass–air system with an index contrast of 2.8:1, the optimized square lattice CARPC exhibits a significantly larger normalized CPBG of about 13.50%, though the use of triangular lattice CARPC is unable to enhance the CPBG. It is almost twice as large as our previously reported result [IEEE J. Sel. Top. Quantum Electron. 22, 4900108(2016)]. Moreover, the CPBG of the square-lattice CARPC could remain until an index contrast as low as 2.24:1. The result not only favors wideband CPBG applications for index contrast systems near 2.8:1, but also makes various optical applications that are dependent on CPBG possible for more widely refractive index contrast systems.

Semiconductor UV photonics: feature introduction

Semiconductor UV photonics research has emerged as one of the most heavily invested areas among semiconductor photonics research due to numerous crucial applications such as sterilization,sensing,curing,and communication.The feature issue disseminates nine timely original research and two review papers from leading research groups and companies,covering most frontiers of the semiconductor UV photonics research,from epitaxy,device physics and design,nanostructures,fabrication,packaging,reliability,and application for light-emitting diodes,laser diodes,and photodetectors.

Etching-free pixel definition in InGaN green micro-LEDs

The traditional plasma etching process for defining micro-LED pixels could lead to significant sidewall damage.Defects near sidewall regions act as non-radiative recombination centers and paths for current leakage,significantly deteriorating device performance.In this study,we demonstrated a novel selective thermal oxidation(STO)method that allowed pixel definition without undergoing plasma damage and subsequent dielectric passivation.Thermal annealing in ambient air oxidized and reshaped the LED structure,such as p-layers and InGaN/GaN multiple quantum wells.Simultaneously,the pixel areas beneath the pre-deposited SiO_(2)layer were selectively and effectively protected.It was demonstrated that prolonged thermal annealing time enhanced the insulating properties of the oxide,significantly reducing LED leakage current.Furthermore,applying a thicker SiO_(2)protective layer minimized device resistance and boosted device efficiency effectively.Utilizing the STO method,InGaN green micro-LED arrays with 50-,30-,and 10-μm pixel sizes were manufactured and characterized.The results indicated that after 4 h of air annealing and with a 3.5-μm SiO_(2)protective layer,the 10-μm pixel array exhibited leakage currents density 1.2×10^(-6)A/cm^(2)at-10 V voltage and a peak on-wafer external quantum efficiency of~6.48%.This work suggests that the STO method could become an effective approach for future micro-LED manufacturing to mitigate adverse LED efficiency size effects due to the plasma etching and improve device efficiency.Micro-LEDs fabricated through the STO method can be applied to micro-displays,visible light communication,and optical interconnect-based memories.Almost planar pixel geometry will provide more possibilities for the monolithic integration of driving circuits with micro-LEDs.Moreover,the STO method is not limited to micro-LED fabrication and can be extended to design other III-nitride devices,such as photodetectors,laser diodes,high-electron-mobility transistors,and Schottky barrier diodes.