Raman spectroscopy plays a crucial role in biochemical analysis.Recently,superhydrophobic surface-enhanced Raman scattering(SERS)substrates have enhanced detection limits by concentrating target molecules into small a...Raman spectroscopy plays a crucial role in biochemical analysis.Recently,superhydrophobic surface-enhanced Raman scattering(SERS)substrates have enhanced detection limits by concentrating target molecules into small areas.However,due to the wet transition phenomenon,further reduction of the droplet contact area is prevented,and the detection limit is restricted.This paper proposes a simple method involving femtosecond laser-induced forward transfer for preparing a hybrid superhydrophilic–superhydrophobic SERS(HS-SERS)substrate by introducing a superhydrophilic pattern to promote the target molecules to concentrate on it for ultratrace detection.Furthermore,the HS-SERS substrate is heated to promote a smaller concentrated area.The water vapor film formed by the contact of the solution with the substrate overcomes droplet collapse,and the target molecules are completely concentrated into the superhydrophilic region without loss during evaporation.Finally,the concentrated region is successfully reduced,and the detection limit is enhanced.The HS-SERS substrate achieved a final contact area of 0.013mm2,a 12.1-fold decrease from the unheated case.The reduction of the contact area led to a detection limit concentration as low as 10−16 M for a Rhodamine 6G solution.In addition,the HS-SERS substrate accurately controlled the size of the concentrated areas through the superhydrophilic pattern,which can be attributed to the favorable repeatability of the droplet concentration results.In addition,the preparation method is flexible and has the potential for fluid mixing,fluid transport,and biochemical sensors,etc.展开更多
In recent years,the incidence of myopia has increased at an alarming rate among children and adolescents in China.The exploration of an effective prevention and control method for myopia is in urgent need.With the dev...In recent years,the incidence of myopia has increased at an alarming rate among children and adolescents in China.The exploration of an effective prevention and control method for myopia is in urgent need.With the development of information technology in the past decade,artificial intelligence with the Internet of Things technology(AIoT)is characterized by strong computing power,advanced algorithm,continuous monitoring,and accurate prediction of long-term progression.Therefore,big data and artificial intelligence technology have the potential to be applied to data mining of myopia etiology and prediction of myopia occurrence and development.More recently,there has been a growing recognition that myopia study involving AIoT needs to undergo a rigorous evaluation to demonstrate robust results.展开更多
PML nuclear bodies(NBs),which are increasingly recognized as the central hub of many cellular signaling events,are superassembled spherical complexes with diameters of 0.1–2mm.Recent studies reveal that RING tetramer...PML nuclear bodies(NBs),which are increasingly recognized as the central hub of many cellular signaling events,are superassembled spherical complexes with diameters of 0.1–2mm.Recent studies reveal that RING tetramerization and B1-box polymerization are key factors to the overall PML NBs assembly.The productive RBCC oligomerization allows subsequent PML biogenesis steps,including the PML auto-sumoylation and partners recruitment via SUMO–SIM interactions.In promyelocytic leukemia,the oncoprotein PML/RARa(P/R)inhibits PML NBs assembly and leads to a full-fledged leukemogenesis.In this review,we review the recent progress in PML and acute promyelocytic leukemia fields,highlighting the protein oligomerization as an important direction of future targeted therapy.展开更多
基金This work was supported by the National Key R&D Program of China(2018YFB1107200)the National Natural Science Foundation of China(51675049)+1 种基金the Natural Science Foundation of Beijing Municipality(3172027)the Young Elite Scientists Sponsorship Program(2016QNRC001).
文摘Raman spectroscopy plays a crucial role in biochemical analysis.Recently,superhydrophobic surface-enhanced Raman scattering(SERS)substrates have enhanced detection limits by concentrating target molecules into small areas.However,due to the wet transition phenomenon,further reduction of the droplet contact area is prevented,and the detection limit is restricted.This paper proposes a simple method involving femtosecond laser-induced forward transfer for preparing a hybrid superhydrophilic–superhydrophobic SERS(HS-SERS)substrate by introducing a superhydrophilic pattern to promote the target molecules to concentrate on it for ultratrace detection.Furthermore,the HS-SERS substrate is heated to promote a smaller concentrated area.The water vapor film formed by the contact of the solution with the substrate overcomes droplet collapse,and the target molecules are completely concentrated into the superhydrophilic region without loss during evaporation.Finally,the concentrated region is successfully reduced,and the detection limit is enhanced.The HS-SERS substrate achieved a final contact area of 0.013mm2,a 12.1-fold decrease from the unheated case.The reduction of the contact area led to a detection limit concentration as low as 10−16 M for a Rhodamine 6G solution.In addition,the HS-SERS substrate accurately controlled the size of the concentrated areas through the superhydrophilic pattern,which can be attributed to the favorable repeatability of the droplet concentration results.In addition,the preparation method is flexible and has the potential for fluid mixing,fluid transport,and biochemical sensors,etc.
基金The Science and Technology Planning Projects of Guangdong Province(Grant No.2018B010109008)National Key R&D Program of China(Grant No.2018YFC0116500).
文摘In recent years,the incidence of myopia has increased at an alarming rate among children and adolescents in China.The exploration of an effective prevention and control method for myopia is in urgent need.With the development of information technology in the past decade,artificial intelligence with the Internet of Things technology(AIoT)is characterized by strong computing power,advanced algorithm,continuous monitoring,and accurate prediction of long-term progression.Therefore,big data and artificial intelligence technology have the potential to be applied to data mining of myopia etiology and prediction of myopia occurrence and development.More recently,there has been a growing recognition that myopia study involving AIoT needs to undergo a rigorous evaluation to demonstrate robust results.
基金supported by research grants 81970132,81770142,81370620,81570120,31070645,81800144,and 31800642 from National Natural Science Foundation of Chinaa research grant 20152504 from Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support+4 种基金The Program for Professor of Special Appointment(Eastern Scholar)at Shanghai Institute of Higher Learninga research grant 11JC1407200 from SMSTCa research grant 12ZZ109 from SMEProgram for New Century Excellent Talents in University(NCET-10–9571)Samuel Waxman Cancer Research Foundation.
文摘PML nuclear bodies(NBs),which are increasingly recognized as the central hub of many cellular signaling events,are superassembled spherical complexes with diameters of 0.1–2mm.Recent studies reveal that RING tetramerization and B1-box polymerization are key factors to the overall PML NBs assembly.The productive RBCC oligomerization allows subsequent PML biogenesis steps,including the PML auto-sumoylation and partners recruitment via SUMO–SIM interactions.In promyelocytic leukemia,the oncoprotein PML/RARa(P/R)inhibits PML NBs assembly and leads to a full-fledged leukemogenesis.In this review,we review the recent progress in PML and acute promyelocytic leukemia fields,highlighting the protein oligomerization as an important direction of future targeted therapy.
