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数据驱动的人体正常和癌症组织中糖基磷脂酰肌醇锚定蛋白(GPI-AP)相关基因表达谱的综合分析
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作者 孔维泽 柳艺石 +1 位作者 高晓冬 藤田盛久 《遗传》 CAS CSCD 北大核心 2023年第8期669-683,共15页
人体细胞中含有超过146种GPI锚定蛋白(glycosylphosphatidylinositol-anchored protein,GPI-AP),包括受体、配体、粘附分子和酶等。这些蛋白通过GPI锚定在细胞膜表面的脂筏中,发挥多种重要生物学功能。目前,已经对GPI锚定蛋白的生物合... 人体细胞中含有超过146种GPI锚定蛋白(glycosylphosphatidylinositol-anchored protein,GPI-AP),包括受体、配体、粘附分子和酶等。这些蛋白通过GPI锚定在细胞膜表面的脂筏中,发挥多种重要生物学功能。目前,已经对GPI锚定蛋白的生物合成开展了大量研究,其中GPI-AP的生物合成包括至少20步反应,已鉴定出超过40个GPI-AP合成相关基因,但是仍缺乏对于GPI-AP相关基因在正常组织与癌症组织中表达调控的研究。本研究利用来自于TCGA数据库和GTEx portal的基因表达数据,同时结合使用GlycoMaple糖基化通路分析工具,对正常组织与癌症组织中参与GPI-AP合成和编码GPI-AP的基因的表达情况进行了全面分析。研究发现,与正常组织相比,在早期胶质瘤、多形性胶质母细胞瘤、胰腺癌、睾丸生殖细胞癌、原发性皮肤黑色素瘤和转移性皮肤黑色素瘤中,参与GPI-AP合成的基因表达发生了显著变化,其中PIGY在这6种癌症中表达量均有上升。此外,GPI锚定蛋白编码基因CD14在这6种癌症中的表达量上升。GPI锚定蛋白编码基因GAS1、GPC2、GPC4仅在早期胶质瘤和多形性胶质母细胞瘤中表达量上升,说明部分GPI锚定蛋白如GAS1等可以作为早期胶质瘤和多形性胶质母细胞瘤生物标志物。本研究为GPI-AP相关基因在正常组织与癌症组织中的表达情况提供了新的见解,为GPI-AP作为生物标志物的开发打下了坚实基础。 展开更多
关键词 糖基磷脂酰肌醇锚定蛋白(GPI-AP) TCGA 癌症 胶质瘤 标志物
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Investigation of TLP's hydrodynamic response with different tendon connection angles
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作者 Yang Yu Aaron Hardowar +2 位作者 Jianxing Yu Shuai Hao xiaodong gao 《Theoretical & Applied Mechanics Letters》 CAS CSCD 2018年第4期291-297,301,共8页
This paper aims to investigate the hydrodynamic behavior of a tension leg platform(TLP)when the tendon connection angles are varied at 90°,70°,50°,and 30°.Three different types of loading condition... This paper aims to investigate the hydrodynamic behavior of a tension leg platform(TLP)when the tendon connection angles are varied at 90°,70°,50°,and 30°.Three different types of loading conditions are applied to the TLP.Conditions include 100-year hurricane storm period,regular waves and no loading.The TLP displayed major response in the pitch degree of freedom.A maximum reduction of 14% in pitch rotation is achieved when 100-year hurricane storm conditions are applied to the TLP.This occurred in 0°loadings at 30°tendon connection angle as compared to 90°tendon connection angle.Reduction in pitch rotation is also achieved in the regular wave loadings.A maximum of 9% in pitch rotation is achieved during 0°wave loading at 30°tendon connection angle as compared to 90°.When the tendon connection angle is reduced from 90°to 30°,the natural frequency of the TLP increased both in pitch and yaw degrees of freedom by 2.55% and 2.40%,respectively. 展开更多
关键词 TENDON CONNECTION IRREGULAR WAVES Regular WAVES Pitch Yaw
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Interactive effect of thermal aging and proton irradiation on microstructural evolution and hardening ofδ-ferrite in 308L stainless steel weld metal
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作者 xiaodong gao xiaodong Lin +6 位作者 Tao Guo Lining Xu Yaolei Han Baolong Jiang Xingyuan Mei Qunjia Peng Lijie Qiao 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2024年第3期140-155,共16页
In the harsh service environment of high temperature and intense neutron irradiation in water-cooled nuclear reactors,the austenitic stainless steel weld overlay cladding on the inner surface of the reactor pressure v... In the harsh service environment of high temperature and intense neutron irradiation in water-cooled nuclear reactors,the austenitic stainless steel weld overlay cladding on the inner surface of the reactor pressure vessel suffers from thermal aging and irradiation damage simultaneously,which can induce microstructural evolution and hardening of the material.Since it is quite difficult to achieve this simul-taneous process out of the pile,two kinds of combined experiments,i.e.,post-irradiation thermal aging and post-aging irradiation were performed on 308 L stainless steel weld metals in this work.The interactive effect of thermal aging and proton irradiation on microstructural evolution and hardening ofδ-ferrite in 308 L weld metal was investigated by combining atom probe tomography,transmission elec-tron microscopy and nanoindentation tests.The results revealed that thermal aging could eliminate the dislocation loops induced by irradiation and affect the phase transition process by accelerating spinodal decomposition and G-phase precipitation,thus enhancing hardening of irradiatedδ-ferrite.For the effect of irradiation on the microstructure and hardening of thermally agedδ-ferrite,however,intensive collision cascades can intensify G-phase precipitation and dislocation loop formation but decrease spinodal decomposition,leading to a limited effect on hardening of thermally agedδ-ferrite.Furthermore,the interaction of thermal aging and irradiation can promote G-phase precipitation.Meanwhile,the interaction can causeδ-ferrite hardening,which is mainly influenced by spinodal decomposition,followed by G-phase and dislocation loops,where spinodal decomposition and G-phase cause hardening by inducing strain fields. 展开更多
关键词 Austenitic stainless steel weld metals Thermal aging Proton irradiation Interaction Microstructural evolution HARDENING
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The effect of lateral growth of self-assembled GaN microdisks on UV lasing action
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作者 Zhiwei Si Zongliang Liu +9 位作者 Xiaoxuan Wang Chunxiang Xu Wei Lin Xiaoxuan Luo Feng Li Xiaoming Dong Shunan Zheng xiaodong gao Jianfeng Wang Ke Xu 《Nano Research》 SCIE EI CSCD 2023年第8期11096-11106,共11页
There are significant differences in the extent of impurity incorporation on different crystallographic directions of GaN microstructures,and the impurity-related deep energy level behavior will have a significant imp... There are significant differences in the extent of impurity incorporation on different crystallographic directions of GaN microstructures,and the impurity-related deep energy level behavior will have a significant impact on device performance.However,a comprehensive understanding of the effect of lateral growth on device performance has not been achieved due to the lack of comprehensive spatial distribution characterization of the optical behavior and impurity incorporation in GaN microstructures.We present a comprehensive study of the optical behavior and growth mechanism of self-assembled GaN microdisks using nanoscale spatially resolved cathodoluminescence(CL)mapping.We have found a clear growth orientation-dependent optical behavior of the lateral and vertical growth sectors of self-assembled GaN microcrystals.The lateral growth sector,i.e.,the{101¯1}-growth sector,forms six side facets of the microdisk and shows significant near-bandgap emission(NBE)and weak deep energy level luminescence.Cavity effect enhanced emission was found for the first time in such a truncated hexagonal Na-flux GaN microdisk system with an ultra-smooth surface(Ra<0.7 nm)and low stress.The self-assembled microdisk shows significant ultraviolet(UV)lasing action(main lasing peak wavelength 370.9 nm,quality factor 1278,threshold 6×10^(4)μJ/cm^(2))under pulsed optical pumping.We believe that the appearance of UV lasing action may be related to the light limitation on the six side facets of the lateral growth of the GaN microdisk,the high structural quality,the low content of deep energy level defects,the low surface roughness,and the low stress. 展开更多
关键词 homogeneous lateral epitaxy spatially resolved cathodoluminescence deep energy level GaN microdisk LASING
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构建酿酒酵母细胞工厂高效合成摩尔酸
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作者 高惠芳 邵明龙 +5 位作者 周武林 张显 杨套伟 徐美娟 高晓冬 饶志明 《微生物学报》 CAS CSCD 北大核心 2021年第11期3506-3519,共14页
【目的】摩尔酸作为齐墩果烷型三萜化合物具有抗HIV、抗炎等多种生物学活性,其前体物质是计曼尼醇,本研究基于合成生物学策略构建酿酒酵母细胞工厂高效合成摩尔酸。【方法】运用CRISPR/Cas9技术,首先分别整合不同来源的氧化鲨烯环化酶(O... 【目的】摩尔酸作为齐墩果烷型三萜化合物具有抗HIV、抗炎等多种生物学活性,其前体物质是计曼尼醇,本研究基于合成生物学策略构建酿酒酵母细胞工厂高效合成摩尔酸。【方法】运用CRISPR/Cas9技术,首先分别整合不同来源的氧化鲨烯环化酶(OSCs),筛选高产计曼尼醇底盘细胞;进一步异源表达长春花来源的细胞色素P450氧化酶(CYP716AL1)和麻风树来源的细胞色素P450还原酶(JcCPR),构建摩尔酸生物合成途径;并通过CYP716AL1和不同来源的CPR适配研究以及过表达甲羟戊酸(MVA)代谢途径中关键酶的方式提高摩尔酸的产量。【结果】整合苹果来源的氧化鲨烯环化酶MdOSC获得的重组菌株计曼尼醇产量最高,达68.3 mg/L;以此为底盘细胞进一步整合CYP716AL1和JcCPR实现了摩尔酸的生物合成,产量为15.0 mg/L;共表达CYP716AL1和拟南芥来源的CPR获得的重组菌株摩尔酸产量最高,达到24.3 mg/L;最后过表达MVA代谢途径中的关键酶法呢基焦磷酸合酶(ERG20)和鲨烯环氧酶(ERG1),获得的重组菌株摩尔酸产量高达34.1 mg/L。【结论】本研究实现了摩尔酸的高效生物合成,为构建高产齐墩果烷型三萜酿酒酵母细胞工厂提供了理论和技术依据。 展开更多
关键词 三萜 计曼尼醇 摩尔酸 合成生物学 酿酒酵母
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筛选抑制酿酒酵母生长的人类基因 被引量:1
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作者 王彩虹 高晓冬 中西秀树 《微生物学报》 CAS CSCD 北大核心 2018年第11期1884-1896,共13页
【目的】基于人类基因文库,构建一个筛选抑制酿酒酵母生长的人类基因的方法,并运用此方法筛选含有生长抑制性人源蛋白质的酿酒酵母,用于分析人类基因的生理功能及其抑制剂的寻找。【方法】利用Gateway^(TM)重组技术将人类蛋白质编码基... 【目的】基于人类基因文库,构建一个筛选抑制酿酒酵母生长的人类基因的方法,并运用此方法筛选含有生长抑制性人源蛋白质的酿酒酵母,用于分析人类基因的生理功能及其抑制剂的寻找。【方法】利用Gateway^(TM)重组技术将人类蛋白质编码基因构建到酿酒酵母表达质粒中。得到的质粒分别转化酿酒酵母细胞中,分析哪些基因的表达会抑制酿酒酵母的生长,并用绿色荧光蛋白标签对典型候选基因在酿酒酵母中的定位进行观察。【结果与结论】本研究建立了抑制酿酒酵母生长的人类基因的筛选方法,并运用此方法成功地从2991个人类蛋白质编码基因中筛选到29个显著抑制酿酒酵母生长的基因。其中一些是引起人类疾病的致病基因。例如,PDLIM4参与到骨质疏松症和前列腺癌的形成和发展,但其生理功能尚不清楚。我们的研究可能为揭示这些候选基因的功能和调节机制提供新的途径。 展开更多
关键词 筛选 人类基因 酿酒酵母 抑制生长 荧光定位
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