Esophagus squamous cell carcinoma(ESCC) is one of the most aggressive malignant tumors in the world. Our previous data demonstrates that oncoprotein MUC1 is related with metastasis and poor outcome of ESCC. However, a...Esophagus squamous cell carcinoma(ESCC) is one of the most aggressive malignant tumors in the world. Our previous data demonstrates that oncoprotein MUC1 is related with metastasis and poor outcome of ESCC. However, alteration of MUC1 in ESCC remains unclear. Using ONCOMINE and COSMIC databases, we analyzed MUC1 gene copy numbers and gene mutations and found that MUC1 had high expression level but few gene mutations in ESCC. Further study of ESCC samples indicated that MUC1 O-glycosylation levels were higher in tumor tissues than that in para-carcinoma tissues in 10 of 14 pairs of ESCC samples. Moreover, we verified a potential link between MUC1 O-glycosylation and C1 GALT1, which was further supported by IHC analysis on 38 ESCC and 19 para-carcinoma samples. More importantly, co-expression of MUC1 Oglycosylation and C1 GALT1 presented positive correlations with both lymph node metastasis and survival time of ESCC patients. Our work collectively indicates that C1 GALT1 is associated with O-glycosylated MUC1 in ESCC, not only suggesting a diagnostic significance of C1 GALT1 and MUC1 O-glycosylation in ESCC, but also opening novel insights into targeting C1 GALT1 and MUC1 O-glycosylation to suppress ESCC cells metastasis in patients.展开更多
By homologous expressed sequence tag (EST) searching, one EST (GenBank: W29095) was obtained, which shows 75% identity in 435 bp overlap with the coding sequence of mouse Cacng2 gene. A 1 545 bp cDNA fragment was obta...By homologous expressed sequence tag (EST) searching, one EST (GenBank: W29095) was obtained, which shows 75% identity in 435 bp overlap with the coding sequence of mouse Cacng2 gene. A 1 545 bp cDNA fragment was obtained from the nested polymerase chain reaction (PCR) and rapid applification of cDNA end (RACE) reaction in the human brain prefrontal cortex cDNA library and the human brain Ready cDNA with the primers designed on W29095. The fragment contained a 948-bp open reading frame (ORF) encoding 315 amino acids, and was named CACNG3. As it was identical to a BAC clone (GenBank: AC004125) from chromosome 16p12-p13.1, the CACNG3 gene was mapped to human chromosome 16p12-p13.1, and the coding region was composed of 4 exons. Reverse transcription PCR (RT-PCR) analysis showed that the CACNG3 gene expressed in human adult brain and fetal brain. Single strand comformation polymorphism (SSCP) analysis was performed in 3 pedigrees with autosomal recessive retinitis pigmentosa, 8 pedigrees with autosomal展开更多
Dear Editor,Mucin 1(MUC1)contains N-and C-subunit that forms a heterodimer on the apical surface of luminal epithelial cells.Nevertheless,MUC1 is aberrantly overexpressed in various cancers,such as breast cancer,lung ...Dear Editor,Mucin 1(MUC1)contains N-and C-subunit that forms a heterodimer on the apical surface of luminal epithelial cells.Nevertheless,MUC1 is aberrantly overexpressed in various cancers,such as breast cancer,lung cancer,and prostatic cancer,which critically contributes to tumorigenesis and poor clinical outcomes.1 Several strategies have been developed for targeted-inhibition of MUC1 including vaccines,monoclonal antibodies(MAb),and polypeptide.展开更多
基金supported by the National Natural Science Foundation of China (81472461)
文摘Esophagus squamous cell carcinoma(ESCC) is one of the most aggressive malignant tumors in the world. Our previous data demonstrates that oncoprotein MUC1 is related with metastasis and poor outcome of ESCC. However, alteration of MUC1 in ESCC remains unclear. Using ONCOMINE and COSMIC databases, we analyzed MUC1 gene copy numbers and gene mutations and found that MUC1 had high expression level but few gene mutations in ESCC. Further study of ESCC samples indicated that MUC1 O-glycosylation levels were higher in tumor tissues than that in para-carcinoma tissues in 10 of 14 pairs of ESCC samples. Moreover, we verified a potential link between MUC1 O-glycosylation and C1 GALT1, which was further supported by IHC analysis on 38 ESCC and 19 para-carcinoma samples. More importantly, co-expression of MUC1 Oglycosylation and C1 GALT1 presented positive correlations with both lymph node metastasis and survival time of ESCC patients. Our work collectively indicates that C1 GALT1 is associated with O-glycosylated MUC1 in ESCC, not only suggesting a diagnostic significance of C1 GALT1 and MUC1 O-glycosylation in ESCC, but also opening novel insights into targeting C1 GALT1 and MUC1 O-glycosylation to suppress ESCC cells metastasis in patients.
文摘By homologous expressed sequence tag (EST) searching, one EST (GenBank: W29095) was obtained, which shows 75% identity in 435 bp overlap with the coding sequence of mouse Cacng2 gene. A 1 545 bp cDNA fragment was obtained from the nested polymerase chain reaction (PCR) and rapid applification of cDNA end (RACE) reaction in the human brain prefrontal cortex cDNA library and the human brain Ready cDNA with the primers designed on W29095. The fragment contained a 948-bp open reading frame (ORF) encoding 315 amino acids, and was named CACNG3. As it was identical to a BAC clone (GenBank: AC004125) from chromosome 16p12-p13.1, the CACNG3 gene was mapped to human chromosome 16p12-p13.1, and the coding region was composed of 4 exons. Reverse transcription PCR (RT-PCR) analysis showed that the CACNG3 gene expressed in human adult brain and fetal brain. Single strand comformation polymorphism (SSCP) analysis was performed in 3 pedigrees with autosomal recessive retinitis pigmentosa, 8 pedigrees with autosomal
基金This work was supported by National Natural Science Foundation of China(grant numbers 82073111 and 81874197)to L.H.and Chinese Universities Scientific FundCAMS Innovation Fund for Medical Sciences(2019-I2M-5-051)to G.C.
文摘Dear Editor,Mucin 1(MUC1)contains N-and C-subunit that forms a heterodimer on the apical surface of luminal epithelial cells.Nevertheless,MUC1 is aberrantly overexpressed in various cancers,such as breast cancer,lung cancer,and prostatic cancer,which critically contributes to tumorigenesis and poor clinical outcomes.1 Several strategies have been developed for targeted-inhibition of MUC1 including vaccines,monoclonal antibodies(MAb),and polypeptide.
