Multi-environment BSA-seq using large F3 populations is able to achieve reliable QTL mapping with high power and resolution: An experimental demonstration in rice

Bulked-segregant analysis by deep sequencing(BSA-seq) is a widely used method for mapping QTL(quantitative trait loci) due to its simplicity, speed, cost-effectiveness, and efficiency. However, the ability of BSA-seq to detect QTL is often limited by inappropriate experimental designs, as evidenced by numerous practical studies. Most BSA-seq studies have utilized small to medium-sized populations, with F2populations being the most common choice. Nevertheless, theoretical studies have shown that using a large population with an appropriate pool size can significantly enhance the power and resolution of QTL detection in BSA-seq, with F_(3)populations offering notable advantages over F2populations. To provide an experimental demonstration, we tested the power of BSA-seq to identify QTL controlling days from sowing to heading(DTH) in a 7200-plant rice F_(3)population in two environments, with a pool size of approximately 500. Each experiment identified 34 QTL, an order of magnitude greater than reported in most BSA-seq experiments, of which 23 were detected in both experiments, with 17 of these located near41 previously reported QTL and eight cloned genes known to control DTH in rice. These results indicate that QTL mapping by BSA-seq in large F_(3)populations and multi-environment experiments can achieve high power, resolution, and reliability.

Generation and selection of immunized Fab phage display library against human B cell lymphoma

与 B 房间淋巴瘤在病人的管理把单音的同种细胞的抗体用作指向的治疗的赞同为这组病人导致了新治疗选择。由传统的 hybridoma 技术的单音的同种细胞的抗体的生产是昂贵的，并且结果鼠科的抗体经常有触发人的反老鼠抗体(HAMA ) 反应的劣势。因此，噬菌体产生的重组体 Fab 抗体显示技术能是在管理 B 房间淋巴瘤的一种合适的选择。在这研究，我们从与人的 B 淋巴瘤房间使免疫的 BALB/c 老鼠的怒气房间提取了全部的 RNA，并且使用了 RT-PCR 为 kappa 光链和重链的 Fd 碎片放大 cDNAscoding。在适当限制文摘以后，这些 cDNA 碎片连续地被插入到噬菌体构造一个使免疫的 Fab 噬菌体显示图书馆的中间的向量 pComb3H-SS。构造图书馆的差异是近似 1.94x10 (7 ) 。跟随五轮简历 panning，可溶的 Fab 抗体从 ELISA 识别的积极克隆被生产。从八积极克隆， FabC06， FabC21， FabC43 andFabC59 为顺序分析被选择。在氨基酸顺序的水平，可变的重域(V (H)) 和可变的轻域(V (L)) 被发现分享 88-92% 和 89-94% 相同，序列分别地由相应鼠科的细菌行基因编码了。而且，有 B 房间淋巴瘤的膜蛋白质的反应被 immunohistochemistryand 表明西方的弄污。这些使免疫的 Fab 抗体可以为 B 房间淋巴瘤的进一步的学习提供一个珍贵工具并且能也贡献疾病治疗的改进。

Improved bias proportional navigation with multiple constraints for guide ammunition

According to the infrared guidance ammunition(GA)attacking non-maneuvering targets on the ground or sea level,an improved bias proportional navigation(IBPN) is put forward,which can meet the constraints of the impact angle and the angle of attack(AOA). The motion equations and the collision triangle for the GA and the target are established in the two-dimensional plane. In accordance with the collision triangle, the integral value of the bias term is solved and BPN is designed on the basis of the relative velocity. To ensure the new method can be solved, closedloop equation of the IBPN is deduced. Considering the limitation of the AOA and the seeker angle, a four-phase IBPN is improved by setting different phases of the bias term. At the same time, the guidance law will make the impact angle achieve the desired angle and the normal acceleration also converges to zero. The simulation results show that the improved guidance law can be applied to various flight tasks and has great potential for engineering applications.

Extraction Process of Total Flavonoids from Eucommiae Cortex and Its in vitro Antioxidant Activity

[Objectives]To optimize the extraction process of total flavonoids from Eucommiae Cortex,establish the extraction and content determination methods of its medicinal materials,and study its in vitro antioxidant activity.[Methods]The total flavonoids from Eucommiae Cortex were extracted by reflux extraction method,and the effects of extraction method,extraction solvent concentration,extraction volume,and extraction time on the total flavonoids content of the medicinal materials were explored through the single factor experiment.Orthogonal experiment was carried out to optimize the extraction process conditions,and the optimal extraction process for total flavonoids from Eucommiae Cortex was screened out.The total antioxidant capacity index was used to determine the free radical scavenging ability of the total flavonoids from Eucommiae Cortex.[Results]The optimal extraction process of total flavonoids from Eucommiae Cortex was 50%ethanol,1∶40 solid-to-liquid-ratio,and 1.5 h reflux extraction time.Through the antioxidant experiment in vitro,it is found that the total flavonoids from Eucommiae Cortex have a higher scavenging ability for the total antioxidant capacity,and there is a certain positive correlation with the mass concentration of total flavonoids.[Conclusions]This method can effectively determine the total flavonoids content of Eucommiae Cortex medicinal material,and provide a certain scientific basis for the quality standard research of the medicinal material.The total flavonoids from Eucommiae Cortex have good in vitro antioxidant activity.And the method for extracting total flavonoids from Eucommiae Cortex medicinal material in this experiment has good repeatability,high stability and feasibility.

Research Progress on Antitumor Effect and Mechanism of Panax quinquefolium Linn.

Panax quinquefolium Linn.,belonging to Panax,Araliaceae,contains a variety of active ingredients.In recent years,many studies have shown that P.quinquefolium Linn.has high value and development prospect in inhibiting colon cancer,lung cancer and breast cancer.Recent advances in studies on chemical components,antitumor effects and mechanisms of P.quinquefolium Linn.are reviewed in the paper.

情景模拟训练结合Miller金字塔教学对手术室专科护士应急能力与护理技能影响的研究

目的探讨情景模拟训练结合Miller金字塔教学对手术室专科护士应急能力与护理技能的影响。方法选取2019年6月至2020年6月在电子科技大学医学院附属绵阳医院进行手术室专科轮转的灾害护理学员56人,将其按培训时间先后顺序分为对照组和研究组,每组28人。对照组采用传统带教,研究组采用情景模拟训练结合Miller金字塔教学。培训结束后通过考核专业理论知识和护理技能操作以评价专科护士培训教学效果;采用自拟考核量表评价专科护士培训的应急能力和培训效果的满意度。采用SPSS 22.0进行t检验和卡方检验。结果研究组手术室专科护士培训专业理论知识和护理技能操作考核成绩均高于对照组,差异有统计学意义(P<0.001)。研究组手术室专科护士培训应急能力考核评分均高于对照组,差异有统计学意义(P<0.001)。研究组在提升专科护理操作水平、提升突发事件应急能力、提升团队协作能力、提升分析解决问题能力、提升临床护理决策能力、提升独立思考能力及提升护患沟通能力7个方面的满意度均优于对照组,差异有统计学意义(P<0.05)。结论情景模拟训练结合Miller金字塔教学可提高手术室专科护士的应急能力、护理技能操作及培训满意度。