Objective:To analyze the expression and clinical significance of the zinc finger protein ZNF207 gene in liver hepatocellular carcinoma(LIHC)based on The Cancer Genome Atlas(TCGA)database.Methods:The mRNA sequencing da...Objective:To analyze the expression and clinical significance of the zinc finger protein ZNF207 gene in liver hepatocellular carcinoma(LIHC)based on The Cancer Genome Atlas(TCGA)database.Methods:The mRNA sequencing data of 371 cases of primary liver cancer,50 cases of normal tissues,and 3 cases of recurrent liver cancer were downloaded from the TCGA database.The corresponding clinical information of the 371 cases of hepatocellular carcinoma was subsequently analyzed.The difference in ZNF207 expression between normal and tumor tissues was analyzed using the UALCAN online database.The impact of ZNF207 expression on survival prognosis was assessed using the Kaplan-Meier method in R software.The GO and KEGG pathways of ZNF207 were analyzed.The Cox proportional hazards model was used to evaluate the prognostic factors of patients with LIHC.RT-qPCR was employed to verify the expression of ZNF207 in LIHC cells.Results:ZNF207 was highly expressed in LIHC tissues and HepG2 cells,with a significant difference(P<0.05).Multivariate Cox regression analysis revealed that patients with high ZNF207 expression had a significantly shorter overall survival time compared to those with low ZNF207 expression(HR=1.466,95%CI:1.011-2.126,P<0.05).GO enrichment analysis suggested that ZNF207 may influence the onset and progression of hepatocellular carcinoma by regulating mRNA splicing and mRNA transcription processing through the spliceosome.KEGG pathway enrichment analysis indicated that ZNF207 might affect the onset and progression of hepatocellular carcinoma through mitophagy,mRNA surveillance,homologous recombination,spliceosome,and nuclear-cytoplasmic transport.Conclusion:The expression of ZNF207 may be an independent predictor of the prognosis of patients with LIHC and could influence the development of hepatocellular carcinoma through various gene functions and pathways.It has the potential to serve as a novel molecular marker for predicting the prognosis of hepatocellular carcinoma.展开更多
Recently,increasing evidence suggests that DNA methylation plays a crucial role in fruit ripening.However,the role of DNA methylation in regulating specific traits,such as flavor,remains unclear.Here,we report a role ...Recently,increasing evidence suggests that DNA methylation plays a crucial role in fruit ripening.However,the role of DNA methylation in regulating specific traits,such as flavor,remains unclear.Here,we report a role of DNA methylation in affecting furanone biosynthesis in strawberry.Strawberry quinone oxidoreductase(FaQR)is a key enzyme in furanone biosynthesis.There are four FaQR homologs in strawberry cultivar‘Yuexin’,and one of them,FaQR3,contributes∼50%of FaQR transcripts,indicating a major role of FaQR3 in furanone biosynthesis.Through characterization of levels of DNA methylation and FaQR3 transcript and furanone contents during fruit ripening and after the application of DNA methylation inhibitor,we found that the DNA methylation level of the FaQR3 promoter was negatively correlated with FaQR3 expression and furanone accumulation,suggesting that DNA methylation may be involved in furanone biosynthesis through adjusting FaQR3 expression,and responded to different temperatures consistently.In addition,transient expression of a gene in the RNA-directed DNA methylation(RdDM)pathway,FaAGO4,and enrichment analysis of the 24-nucleotide siRNAs suggested that DNA methylation in the FaQR3 promoter is mediated by the RdDM pathway.Transient RNA interference(RNAi)of FaDML indicated that the demethylation pathway may be involved in regulating furanone accumulation.These findings provide new insights into the role of DNA methylation and demethylation in affecting flavor quality in strawberry during fruit ripening.展开更多
This paper is concerned with some chaotic properties of a kind of coupled map lattices, which is proposed by Kaneko. First, this research discussed the sensitivity, infinite sensitivity, transitivity, accessibility, d...This paper is concerned with some chaotic properties of a kind of coupled map lattices, which is proposed by Kaneko. First, this research discussed the sensitivity, infinite sensitivity, transitivity, accessibility, densely Li-Yorke sensitivity and exact of coupled map lattices. Then, some sufficient conditions under which <img src="Edit_c0fc315a-d176-4c9e-9e41-5cb6bc6d679d.bmp" alt="" /> is Kato chaotic, positive entropy chaotic and Ruelle-Takens chaos are obtained.展开更多
Let <img alt="" src="Edit_6a94976d-35be-4dd4-b74f-d0bf6f497453.png" />be a non-autonomous discrete system and <img alt="" src="Edit_3516e048-3d23-4ae8-81ac-e7e732efbc89...Let <img alt="" src="Edit_6a94976d-35be-4dd4-b74f-d0bf6f497453.png" />be a non-autonomous discrete system and <img alt="" src="Edit_3516e048-3d23-4ae8-81ac-e7e732efbc89.png" /> be a set-valued discrete system induced by it. Where, <img alt="" src="Edit_f67612c1-bbf4-4c21-8b37-7d156ca9502d.png" />is the space formed by all non-empty compact subsets of <em>X</em> endowed with the Hausdorff metric <em>H</em>, <img alt="" src="Edit_cca16788-f64a-47c4-9645-e9c8cf9080fd.png" />is a set-valued mapping sequence induced by <img alt="" src="Edit_5a6d2e7f-3245-4dbd-98ec-dc977e23f3d8.png" />. It is proved that <img alt="" src="Edit_a25ef428-a2ff-46d5-9109-dcc67b57fbec.png" /> is <img alt="" src="Edit_ee8759ba-215c-4088-8590-db9f57eb4a7c.png" />-chaos, then <img alt="" src="Edit_f54b347a-033e-43e2-a3a1-d2fe5ac1f39d.png" />is <img alt="" src="Edit_72a57e59-dc43-4071-b0fe-432e379ddcc9.png" />-chaos. Where <img alt="" src="Edit_97813401-14af-4776-99fe-1e6cd08c3df1.png" />-chaos is denoted to <img alt="" src="Edit_9e2d88b4-7ece-430e-8978-800ff3280799.png" />-sensitive, <img alt="" src="Edit_440b79c1-f679-4571-b14d-6f804f402d75.png" />-sensitive, <img alt="" src="Edit_839b7b55-9961-4d80-b5cb-e7219a0ae871.png" />-transitive, <img alt="" src="Edit_feb0a032-255b-4cbd-b489-6a937c5a287a.png" />-accessible, <img alt="" src="Edit_3ba59c02-6df0-4ae1-8ac0-5c1b620e4a88.png" />-weakly mixing, <img alt="" src="Edit_7362ed03-8686-4cf7-94df-f0933b7abbff.png" />-<em>m</em>-sensitive, infinitely sensitive, or syndetically transitive.展开更多
Ultrasonic extraction conditions of polyphenols from the needles of black pine(Pinus thunbergii) were optimized by single factors analysis and Box-Behnken experiment design. The results showed that both of ethanol con...Ultrasonic extraction conditions of polyphenols from the needles of black pine(Pinus thunbergii) were optimized by single factors analysis and Box-Behnken experiment design. The results showed that both of ethanol concentration and ultrasonic time had a significant effect on polyphenol extraction, and there was significant interaction between any two variables in the three parameters of ethanol concentration, ultrasonic temperature and ultrasonic time. The optimal parameters for polyphenol extraction were the ethanol concentration of 52.67%, ultrasonic temperature at 49.21 ℃and ultrasonic time of 30.76 min. Under these conditions, the actual content of polyphenols was close to the predicted value. Therefore, the process can be used to extract polyphenols from black pine needles.展开更多
The growth and development of rice are closely related with temperature. In order to clarify the mechanism of high temperature resistance in riee, in this study, using high temperature-resistant Indian rice cultivar N...The growth and development of rice are closely related with temperature. In order to clarify the mechanism of high temperature resistance in riee, in this study, using high temperature-resistant Indian rice cultivar N22 as the experimental material, Osa-rniR159c, Osa-miR159d, Osa-miR159f, Osa-miR164d, Osa- rrdR529b and Osa-miR166h-3p obtained by high-throughput sequencing as target genes, the expression patterns of these genes in young panicles of rice under high temperature stress were analyzed by RNA-tailing and primer-extension RT-PCR, which provided theoretical basis for breeding high temperature-resistant rice eultivars.展开更多
Atmospheric pressure micro-discharges in helium gas with a mixture of 0.5%water vapor between two pin electrodes are generated with nanosecond overvoltage pulses.The temporal and spatial characteristics of the dischar...Atmospheric pressure micro-discharges in helium gas with a mixture of 0.5%water vapor between two pin electrodes are generated with nanosecond overvoltage pulses.The temporal and spatial characteristics of the discharges are investigated by means of time-resolved imaging and optical emission spectroscopy with respect to the discharge morphology,gas temperature,electron density,and excited species.The evolution of micro-discharges is captured by intensified CCD camera and electrical properties.The gas temperature is diagnosed by a two-temperature fit to the ro-vibrational OH(A^(2)Σ^(+)–X^П(2),0–0)emission band and is found to remain low at 425 K during the discharge pulses.The profile of electron density performed by the Stark broadening of Ha 656.1-nm and He I 667.8-nm lines is uniform across the discharge gap at the initial of discharge and reaches as high as 10^(23)m^(-3).The excited species of He,OH,and H show different spatio-temporal behaviors from each other by the measurement of their emission intensities,which are discussed qualitatively in regard of their plasma kinetics.展开更多
This paper is concerned with the sensitivity of set-valued discrete systems. Firstly, this paper obtained the equivalence between <img src="Edit_7024f70b-0568-4ca8-a554-c0d05abc0df0.bmp" alt="" ...This paper is concerned with the sensitivity of set-valued discrete systems. Firstly, this paper obtained the equivalence between <img src="Edit_7024f70b-0568-4ca8-a554-c0d05abc0df0.bmp" alt="" />or <img src="Edit_95636a59-7d5d-4b6c-8bd5-f699dd9208df.bmp" alt="" /> and the product system <img src="Edit_c714caaf-0ed9-46bc-b3e1-b0223474a8f5.bmp" alt="" /> in sensitivity, infinite sensitivity, <em>F</em>-sensitivity, (<em>F</em><sub>1</sub>, <em>F</em><sub>2</sub>)-sensitivity. Then, the relation between (<em>X</em>, <em>f</em><sub>1,∞</sub>) or (<em>Y</em>, <em>g</em><sub>1,∞</sub>) and <img src="Edit_55b4ce47-89f3-4476-a8a8-4d4db5a4e8eb.bmp" alt="" /> in ergodic sensitivity is obtained. Where <img src="Edit_a99604c4-2f72-4e75-a998-8057b8790e03.bmp" alt="" /> is the set-valued dynamical system induced by a non-autonomous discrete dynamical system (<em>X</em>, <em>f</em><sub>1,∞</sub>).展开更多
In this study,waste pigeon guano(PG)was re-utilized as an ideal biomass adulterant to improve the photocatalytic activity of the pristine graphitic carbon nitride(g-C_(3)N_(4)).Waste PG and melamine were employed as p...In this study,waste pigeon guano(PG)was re-utilized as an ideal biomass adulterant to improve the photocatalytic activity of the pristine graphitic carbon nitride(g-C_(3)N_(4)).Waste PG and melamine were employed as precursors to fabricate a novel porous multielement-doped g-C_(3)N_(4)(CN-PG-S)nanosheets photocatalyst via in situ thermal polycondensation coupled with thermal exfoliation strategy.The CN-PG-S owned abundant uniformly porous structures,superior conductivity,and excellent photocatalytic abilities,resulting in highly-efficient H_(2)-production(1950μmol g^(–1) h^(–1))and Cr(Ⅵ)reduction(99.1%)un-der visible light,which increased by 22.9-folds and 5.3-folds more than that of pristine g-C_(3)N_(4).The non-metallic(P,S,and O)and metallic elements in CN-PG-S played a crucial role in expanding the visible-light absorption range and promoting the separation-migration of photogenerated electron-hole pairs.And the uniformly porous nanosheet structure of CN-PG-S shortens the diffusion paths of photogenerated carri-ers and exposes more active sites for photocatalytic reactions.This study proposed an eco-friendly re-sources integration strategy of waste PG to prepare excellent CN-PG-S photocatalysts for highly-efficient H_(2)-production and Cr(VI)reduction.展开更多
Cultivated strawberry(Fragaria×ananassa),a world-famous fruit,is subjected to rapid softening during ripening,resulting in a shorter shelf life and severe economic losses during storage and transportation.However...Cultivated strawberry(Fragaria×ananassa),a world-famous fruit,is subjected to rapid softening during ripening,resulting in a shorter shelf life and severe economic losses during storage and transportation.However,there is limited understanding of the molecular mechanism underlying differences in fruit firmness during ripening and postharvest among cultivated strawberries.Here,we explored this molecular mechanism by comparing three cultivated strawberries via firmness measurement,transcriptome analysis,quantitative real-time polymerase chain reaction,and correlation analysis,and revealed FaEXP7,FaPG2,FaPLA,and Faβ-Gal4 as potential softening activators expressed before harvest to determine fruit with more softened texture and shorter shelf life,and that extremely high expression levels of FaCEL1-1 and FaCEL1-3 during ripening might be accelerators to intensify this situation.Additionally,both the enzyme activities of FaCEL and the expression pattern of FaCEL1-3 showed a significantly negative correlation with fruit firmness after harvest,suggesting that FaCEL1-3 might play a key role in promoting strawberry fruit softening not only during ripening but also postharvest.These results showed that the difference in fruit firmness and shelf life among cultivated strawberries was controlled by the temporal expression pattern of a legion of cell wall-associated genes during ripening and postharvest.展开更多
Organic matter(OM) is an important component of sediment. Bioturbation/bioirrigation can remobilize OM and heavy metals that were previously buried in the sediment. The remobilization of buried organic matter, thalliu...Organic matter(OM) is an important component of sediment. Bioturbation/bioirrigation can remobilize OM and heavy metals that were previously buried in the sediment. The remobilization of buried organic matter, thallium(Tl), cadmium(Cd), copper(Cu) and zinc(Zn) from sediment was studied in a laboratory experiment with three organisms: tubificid,chironomid larvae and loach. Results showed that bioturbation/bioirrigation promoted the release of dissolved organic matter(DOM) and dissolved Tl, Cd, Cu and Zn, but only dissolved Zn concentrations decreased with exposure time in overlying water. The presence of organisms altered the compositions of DOM released from sediment,considerably increasing the percentage of fulvic acid-like materials(FA) and humic acidlike materials(HA). In addition, bioturbation/bioirrigation accelerated the growth and reproduction of bacteria to enhance the proportion of soluble microbial byproduct-like materials(SMP). The DOM was divided into five regions in the three-dimensional excitation emission matrix(3 D-EEM), and each part had different correlation with the dissolved heavy metal concentrations. Dissolved Cu had the best correlation with each of the DOM compositions, indicating that Cu in the sediment was in the organic-bound form.Furthermore, the organism type and heavy metal characteristics both played a role in influencing the remobilization of heavy metal.展开更多
The heterogeneous Fenton reaction can generate highly reactive hydroxyl radicals(·OH)from reactions between recyclable solid catalysts and H2O2 at acidic or even circumneutral pH.Hence,it can effectively oxidiz...The heterogeneous Fenton reaction can generate highly reactive hydroxyl radicals(·OH)from reactions between recyclable solid catalysts and H2O2 at acidic or even circumneutral pH.Hence,it can effectively oxidize refractory organics in water or soils and has become a promising environmentally friendly treatment technology.Due to the complex reaction system,the mechanism behind heterogeneous Fenton reactions remains unresolved but fascinating,and is crucial for understanding Fenton chemistry and the development and application of efficient heterogeneous Fenton technologies.Iron-based materials usually possess high catalytic activity,low cost,negligible toxicity and easy recovery,and are a superior type of heterogeneous Fenton catalysts.Therefore,this article reviews the fundamental but important interfacial mechanisms of heterogeneous Fenton reactions catalyzed by iron-based materials..OH,hydroperoxyl radicals/superoxide anions(HO2./O2^-.)and high-valent iron are the three main types of reactive oxygen species(ROS),with different oxidation reactivity and selectivity.Based on the mechanisms of ROS generation,the interfacial mechanisms of heterogeneous Fenton systems can be classified as the homogeneous Fenton mechanism induced by surface-leached iron,the heterogeneous catalysis mechanism,and the heterogeneous reaction-induced homogeneous mechanism.Different heterogeneous Fenton systems catalyzed by characteristic iron-based materials are comprehensively reviewed.Finally,related future research directions are also suggested.展开更多
Biogenic particles discharged by wastewater treatment plants play important roles in receiving water because of the large specific surface area and good mobility of the particles. In this study, the changes induced in...Biogenic particles discharged by wastewater treatment plants play important roles in receiving water because of the large specific surface area and good mobility of the particles. In this study, the changes induced in biogenic particles by natural sunlight were investigated to understand the phototransformation of the particles in the receiving waters. The results showed that photoexposure resulted in significant decreases in the sizes of the biogenic particles but that photoexposure did not impact the zeta potentials. In addition, the photodissolution of biogenic particulate organic matter(POM) led to the generation of biogenic dissolved organic matter(DOM). Characterization using excitation–emission matrix(EEM) spectroscopy showed that photoexposure changed both the shapes and the intensities of the EEM spectra of the biogenic POM; the regions of the T1 and T2 peaks were susceptible to photoexposure. Modeling by parallel factor analysis(PARAFAC) decomposed the EEMs of the biogenic POM into four valid components, i.e., terrestrial or microbial humic-like substances, tryptophan-like proteins, tyrosinelike proteins and hydrophobic proteins. The humic-like substances in the biogenic POM from the effluents were subject to lower decreases(17.0 % and 11.6 %).Throughout the entire incubation time, the PARAFAC components in the biogenic DOM were dominated by elimination mechanisms rather than by production through photodissolution of the biogenic POM.展开更多
利用光热材料吸收太阳能产生局域化的热效应实现太阳能蒸汽转化是一项环境友好型海水淡化和废水净化的技术.本文受自然界中叶片蒸腾作用的启发,设计了一种新型仿生叶片结构,以实现高效的太阳能蒸汽转化.这种新型仿生叶片结构由三个部分...利用光热材料吸收太阳能产生局域化的热效应实现太阳能蒸汽转化是一项环境友好型海水淡化和废水净化的技术.本文受自然界中叶片蒸腾作用的启发,设计了一种新型仿生叶片结构,以实现高效的太阳能蒸汽转化.这种新型仿生叶片结构由三个部分组成:(1)亲水性聚乙烯醇(PVA)海绵层(相当于叶子中的叶肉组织层),具有高孔隙率和低导热性;(2)聚吡咯(PPy)层(相当于叶子中的叶绿素层)负载于PVA海绵表面以吸收太阳能;(3)微/纳米尺度的多孔疏水表面层(相当于叶片中的气孔层).其中,亲水海绵的PVA聚合物网络提供了高效水传输通道的同时实现了内部水蒸发焓的降低;PPy层吸收太阳能并转化为内能加热气液蒸汽界面的水,实现热局部化,大大减小了热损耗;疏水性多孔表面层的边缘效应,为水蒸气提供了高效逃逸通道.基于仿生叶片结构的太阳能蒸汽转化装置在一倍太阳光强(1 kW m^(−2))下蒸发率高达3.09 kg m^(−2) h^(−1).在室外自然光照条件下成功地对盐水和海水进行了脱盐和净化,平均净水速率为1.4 L m^(−2) h^(−1).这种基于仿生叶片结构的太阳能蒸汽转化装置提供了一种极具潜力的高效水净化方法,有助于利用可持续能源缓解全球水危机.展开更多
Fenton oxidation is generally inhibited in the presence of a high concentration of chloride ions.This study investigated the feasibility of using benzoquinone(BQ) and hydroxylamine hydrochloride(HA) as Fenton enha...Fenton oxidation is generally inhibited in the presence of a high concentration of chloride ions.This study investigated the feasibility of using benzoquinone(BQ) and hydroxylamine hydrochloride(HA) as Fenton enhancers for the removal of glycerin from saline water under ambient temperature by accelerating the ferric system.It was found that organics removal was not obviously affected by chloride ions of low concentration(less than 0.1 mol/L),while the mineralization rate was strongly inhibited in the presence of a large amount of chloride ions.In addition,ferric hydrolysis-precipitation was significantly alleviated in the presence of HA and BQ,and HA was more effective in reducing ferric ions into ferrous ions than HA,while the H_2O_2 decomposition rate was higher in the BQ-Fenton system.Electron spin resonance analysis revealed that OH production was reduced in high salinity conditions,while it was enhanced after the addition of HA and BQ(especially HA).This study provided a possible solution to control and alleviate the inhibitory effect of chloride ions on the Fenton process for organics removal.展开更多
The effect of phosphate on adsorption and oxidation of catechol, 1,2-dihydroxybenzene,in a heterogeneous Fenton system was investigated. In situ attenuated total reflectance infrared spectroscopy(ATR-FTIR) was used ...The effect of phosphate on adsorption and oxidation of catechol, 1,2-dihydroxybenzene,in a heterogeneous Fenton system was investigated. In situ attenuated total reflectance infrared spectroscopy(ATR-FTIR) was used to monitor the surface speciation at the nano-Fe_3O_4 catalyst surface. The presence of phosphate decreased the removal rate of catechol and the abatement of dissolved organic compounds, as well as the decomposition of H2O2. This effect of phosphate was mainly due to its strong reaction with surface sites on the iron oxide catalyst. At neutral and acid pH, phosphate could displace the adsorbed catechol from the surface of catalyst and also could compete for surface sites with H2O2. In situ IR spectra indicated the formation of iron phosphate precipitation at the catalyst surface. The iron phosphate surface species may affect the amount of iron atoms taking part in the catalytic decomposition of H2O2 and formation of hydroxyl radicals,and inhibit the catalytic ability of Fe3O4 catalyst. Therefore, phosphate ions worked as stabilizer and inhibitor in a heterogeneous Fenton reaction at the same time, in effect leading to an increase in oxidation efficiency in this study. However, before use of phosphate as pH buffer or H2O2 stabilizer in a heterogeneous Fenton system, the possible inhibitory effect of phosphate on the actual removal of organic pollutants should be fully considered.展开更多
Effluent organic matter(Ef OM) from municipal wastewater treatment plants potentially has a detrimental effect on both aquatic organisms and humans.This study evaluated the removal and transformation of chromophoric...Effluent organic matter(Ef OM) from municipal wastewater treatment plants potentially has a detrimental effect on both aquatic organisms and humans.This study evaluated the removal and transformation of chromophoric dissolved organic matter(CDOM) and fluorescent dissolved organic matter(FDOM) in a full-scale wastewater treatment plant under different seasons.The results showed that bio-treatment was found to be more efficient in removing bulk DOM(in term of dissolved organic carbon,DOC) than CDOM and FDOM,which was contrary to the disinfection process.CDOM and FDOM were selectively removed at various stages during the treatment.Typically,the low molecular weight fractions of CDOM and protein-like FDOM were more efficiently removed during bio-treatment process,whereas the humic-like FDOM exhibited comparable decreases in both bio-treatment and disinfection processes.Overall,the performance of the WWTP was weak in terms of CDOM and FDOM removal,resulting in enrichment of CDOM and FDOM in effluent.Moreover,the total removal of the bulk DOM(P 〈 0.05) and the protein-like FDOM(P 〈 0.05) displayed a significant seasonal variation,with higher removal efficiencies in summer,whereas removal of CDOM and the humic-like FDOM showed little differences between summer and winter.In all,the results provide useful information for understanding the fate and transformation of DOM,illustrating that sub-fractions of DOM could be selectively removed depending on treatment processes and seasonality.展开更多
Single wall carbon nanotube(SWCNT)additives were formulated into(im-Si-graphite composite electrodes and tested in both half cells and full cells with high nickel cathodes.The critical role of small amount of SWCNT ad...Single wall carbon nanotube(SWCNT)additives were formulated into(im-Si-graphite composite electrodes and tested in both half cells and full cells with high nickel cathodes.The critical role of small amount of SWCNT addition(0.2 wt%)was found for significantly improving delithiation capacity,first cycle coulombic efficiency(FCE),and capacity retention.Particularly,Si(10 wt%)-graphite electrode exhibits 560 mAh/g delithiation capacity and 92%FCE at 0.2 C during the first chargedischarge cycle,and 91%capacity retention after 50 cycles(0.5 C)in a half cell.Scanning electron microscope(SEM)was used to illustrate the electrode morphology,compositions and promoting function of the SWCNT additives.In addition,full cells assembled with high nickel-NCM811 cathodes and fim-Si-graphite composite anodes were evaluated for the consistence between half and full cell performance,and the consideration for potential commercial application.Finally,criteria to assess Si-containing anodes are proposed and discussed from an industrial perspective.展开更多
文摘Objective:To analyze the expression and clinical significance of the zinc finger protein ZNF207 gene in liver hepatocellular carcinoma(LIHC)based on The Cancer Genome Atlas(TCGA)database.Methods:The mRNA sequencing data of 371 cases of primary liver cancer,50 cases of normal tissues,and 3 cases of recurrent liver cancer were downloaded from the TCGA database.The corresponding clinical information of the 371 cases of hepatocellular carcinoma was subsequently analyzed.The difference in ZNF207 expression between normal and tumor tissues was analyzed using the UALCAN online database.The impact of ZNF207 expression on survival prognosis was assessed using the Kaplan-Meier method in R software.The GO and KEGG pathways of ZNF207 were analyzed.The Cox proportional hazards model was used to evaluate the prognostic factors of patients with LIHC.RT-qPCR was employed to verify the expression of ZNF207 in LIHC cells.Results:ZNF207 was highly expressed in LIHC tissues and HepG2 cells,with a significant difference(P<0.05).Multivariate Cox regression analysis revealed that patients with high ZNF207 expression had a significantly shorter overall survival time compared to those with low ZNF207 expression(HR=1.466,95%CI:1.011-2.126,P<0.05).GO enrichment analysis suggested that ZNF207 may influence the onset and progression of hepatocellular carcinoma by regulating mRNA splicing and mRNA transcription processing through the spliceosome.KEGG pathway enrichment analysis indicated that ZNF207 might affect the onset and progression of hepatocellular carcinoma through mitophagy,mRNA surveillance,homologous recombination,spliceosome,and nuclear-cytoplasmic transport.Conclusion:The expression of ZNF207 may be an independent predictor of the prognosis of patients with LIHC and could influence the development of hepatocellular carcinoma through various gene functions and pathways.It has the potential to serve as a novel molecular marker for predicting the prognosis of hepatocellular carcinoma.
文摘Recently,increasing evidence suggests that DNA methylation plays a crucial role in fruit ripening.However,the role of DNA methylation in regulating specific traits,such as flavor,remains unclear.Here,we report a role of DNA methylation in affecting furanone biosynthesis in strawberry.Strawberry quinone oxidoreductase(FaQR)is a key enzyme in furanone biosynthesis.There are four FaQR homologs in strawberry cultivar‘Yuexin’,and one of them,FaQR3,contributes∼50%of FaQR transcripts,indicating a major role of FaQR3 in furanone biosynthesis.Through characterization of levels of DNA methylation and FaQR3 transcript and furanone contents during fruit ripening and after the application of DNA methylation inhibitor,we found that the DNA methylation level of the FaQR3 promoter was negatively correlated with FaQR3 expression and furanone accumulation,suggesting that DNA methylation may be involved in furanone biosynthesis through adjusting FaQR3 expression,and responded to different temperatures consistently.In addition,transient expression of a gene in the RNA-directed DNA methylation(RdDM)pathway,FaAGO4,and enrichment analysis of the 24-nucleotide siRNAs suggested that DNA methylation in the FaQR3 promoter is mediated by the RdDM pathway.Transient RNA interference(RNAi)of FaDML indicated that the demethylation pathway may be involved in regulating furanone accumulation.These findings provide new insights into the role of DNA methylation and demethylation in affecting flavor quality in strawberry during fruit ripening.
文摘This paper is concerned with some chaotic properties of a kind of coupled map lattices, which is proposed by Kaneko. First, this research discussed the sensitivity, infinite sensitivity, transitivity, accessibility, densely Li-Yorke sensitivity and exact of coupled map lattices. Then, some sufficient conditions under which <img src="Edit_c0fc315a-d176-4c9e-9e41-5cb6bc6d679d.bmp" alt="" /> is Kato chaotic, positive entropy chaotic and Ruelle-Takens chaos are obtained.
文摘Let <img alt="" src="Edit_6a94976d-35be-4dd4-b74f-d0bf6f497453.png" />be a non-autonomous discrete system and <img alt="" src="Edit_3516e048-3d23-4ae8-81ac-e7e732efbc89.png" /> be a set-valued discrete system induced by it. Where, <img alt="" src="Edit_f67612c1-bbf4-4c21-8b37-7d156ca9502d.png" />is the space formed by all non-empty compact subsets of <em>X</em> endowed with the Hausdorff metric <em>H</em>, <img alt="" src="Edit_cca16788-f64a-47c4-9645-e9c8cf9080fd.png" />is a set-valued mapping sequence induced by <img alt="" src="Edit_5a6d2e7f-3245-4dbd-98ec-dc977e23f3d8.png" />. It is proved that <img alt="" src="Edit_a25ef428-a2ff-46d5-9109-dcc67b57fbec.png" /> is <img alt="" src="Edit_ee8759ba-215c-4088-8590-db9f57eb4a7c.png" />-chaos, then <img alt="" src="Edit_f54b347a-033e-43e2-a3a1-d2fe5ac1f39d.png" />is <img alt="" src="Edit_72a57e59-dc43-4071-b0fe-432e379ddcc9.png" />-chaos. Where <img alt="" src="Edit_97813401-14af-4776-99fe-1e6cd08c3df1.png" />-chaos is denoted to <img alt="" src="Edit_9e2d88b4-7ece-430e-8978-800ff3280799.png" />-sensitive, <img alt="" src="Edit_440b79c1-f679-4571-b14d-6f804f402d75.png" />-sensitive, <img alt="" src="Edit_839b7b55-9961-4d80-b5cb-e7219a0ae871.png" />-transitive, <img alt="" src="Edit_feb0a032-255b-4cbd-b489-6a937c5a287a.png" />-accessible, <img alt="" src="Edit_3ba59c02-6df0-4ae1-8ac0-5c1b620e4a88.png" />-weakly mixing, <img alt="" src="Edit_7362ed03-8686-4cf7-94df-f0933b7abbff.png" />-<em>m</em>-sensitive, infinitely sensitive, or syndetically transitive.
基金Supported by the Policy Guidance Plan of Jiangsu Province(Production and Research Cooperation)(BY2016061-27)National Spark Program(2015GA690284)College Students' Practice Innovation Training Program of Jiangsu Province(201711049094X,201811049048X)
文摘Ultrasonic extraction conditions of polyphenols from the needles of black pine(Pinus thunbergii) were optimized by single factors analysis and Box-Behnken experiment design. The results showed that both of ethanol concentration and ultrasonic time had a significant effect on polyphenol extraction, and there was significant interaction between any two variables in the three parameters of ethanol concentration, ultrasonic temperature and ultrasonic time. The optimal parameters for polyphenol extraction were the ethanol concentration of 52.67%, ultrasonic temperature at 49.21 ℃and ultrasonic time of 30.76 min. Under these conditions, the actual content of polyphenols was close to the predicted value. Therefore, the process can be used to extract polyphenols from black pine needles.
基金Supported by Science and Technology Support Project of 12thFive-Year Plan(2012BAD14B13)Dachuang Fund of Anhui Agricultural University(XJDC2014260)
文摘The growth and development of rice are closely related with temperature. In order to clarify the mechanism of high temperature resistance in riee, in this study, using high temperature-resistant Indian rice cultivar N22 as the experimental material, Osa-rniR159c, Osa-miR159d, Osa-miR159f, Osa-miR164d, Osa- rrdR529b and Osa-miR166h-3p obtained by high-throughput sequencing as target genes, the expression patterns of these genes in young panicles of rice under high temperature stress were analyzed by RNA-tailing and primer-extension RT-PCR, which provided theoretical basis for breeding high temperature-resistant rice eultivars.
基金supported by the National Natural Science Foundation of China(Grant No.51806186)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(Grant No.20KJB140025)+1 种基金the Natural Science Foundation of Jiangsu Province,China(Grant No.BK20181050)the Scientific Research Project for the Introduction Talent of Yancheng Institute of Technology(Grant No.XJR2020)。
文摘Atmospheric pressure micro-discharges in helium gas with a mixture of 0.5%water vapor between two pin electrodes are generated with nanosecond overvoltage pulses.The temporal and spatial characteristics of the discharges are investigated by means of time-resolved imaging and optical emission spectroscopy with respect to the discharge morphology,gas temperature,electron density,and excited species.The evolution of micro-discharges is captured by intensified CCD camera and electrical properties.The gas temperature is diagnosed by a two-temperature fit to the ro-vibrational OH(A^(2)Σ^(+)–X^П(2),0–0)emission band and is found to remain low at 425 K during the discharge pulses.The profile of electron density performed by the Stark broadening of Ha 656.1-nm and He I 667.8-nm lines is uniform across the discharge gap at the initial of discharge and reaches as high as 10^(23)m^(-3).The excited species of He,OH,and H show different spatio-temporal behaviors from each other by the measurement of their emission intensities,which are discussed qualitatively in regard of their plasma kinetics.
文摘This paper is concerned with the sensitivity of set-valued discrete systems. Firstly, this paper obtained the equivalence between <img src="Edit_7024f70b-0568-4ca8-a554-c0d05abc0df0.bmp" alt="" />or <img src="Edit_95636a59-7d5d-4b6c-8bd5-f699dd9208df.bmp" alt="" /> and the product system <img src="Edit_c714caaf-0ed9-46bc-b3e1-b0223474a8f5.bmp" alt="" /> in sensitivity, infinite sensitivity, <em>F</em>-sensitivity, (<em>F</em><sub>1</sub>, <em>F</em><sub>2</sub>)-sensitivity. Then, the relation between (<em>X</em>, <em>f</em><sub>1,∞</sub>) or (<em>Y</em>, <em>g</em><sub>1,∞</sub>) and <img src="Edit_55b4ce47-89f3-4476-a8a8-4d4db5a4e8eb.bmp" alt="" /> in ergodic sensitivity is obtained. Where <img src="Edit_a99604c4-2f72-4e75-a998-8057b8790e03.bmp" alt="" /> is the set-valued dynamical system induced by a non-autonomous discrete dynamical system (<em>X</em>, <em>f</em><sub>1,∞</sub>).
基金This work was financially supported by the National Natural Science Foundation of China(Nos.52070171 and 52030003)China Postdoctoral Science Foundation(Grant No.2022M710138).
文摘In this study,waste pigeon guano(PG)was re-utilized as an ideal biomass adulterant to improve the photocatalytic activity of the pristine graphitic carbon nitride(g-C_(3)N_(4)).Waste PG and melamine were employed as precursors to fabricate a novel porous multielement-doped g-C_(3)N_(4)(CN-PG-S)nanosheets photocatalyst via in situ thermal polycondensation coupled with thermal exfoliation strategy.The CN-PG-S owned abundant uniformly porous structures,superior conductivity,and excellent photocatalytic abilities,resulting in highly-efficient H_(2)-production(1950μmol g^(–1) h^(–1))and Cr(Ⅵ)reduction(99.1%)un-der visible light,which increased by 22.9-folds and 5.3-folds more than that of pristine g-C_(3)N_(4).The non-metallic(P,S,and O)and metallic elements in CN-PG-S played a crucial role in expanding the visible-light absorption range and promoting the separation-migration of photogenerated electron-hole pairs.And the uniformly porous nanosheet structure of CN-PG-S shortens the diffusion paths of photogenerated carri-ers and exposes more active sites for photocatalytic reactions.This study proposed an eco-friendly re-sources integration strategy of waste PG to prepare excellent CN-PG-S photocatalysts for highly-efficient H_(2)-production and Cr(VI)reduction.
基金supported by the Key Research and Development Program of Shandong Province,China(No.2022TZXD0022)the National Natural Science Foundation of China(Nos.32102345 and 32002004)the 111 Project(No.B17039),China.
文摘Cultivated strawberry(Fragaria×ananassa),a world-famous fruit,is subjected to rapid softening during ripening,resulting in a shorter shelf life and severe economic losses during storage and transportation.However,there is limited understanding of the molecular mechanism underlying differences in fruit firmness during ripening and postharvest among cultivated strawberries.Here,we explored this molecular mechanism by comparing three cultivated strawberries via firmness measurement,transcriptome analysis,quantitative real-time polymerase chain reaction,and correlation analysis,and revealed FaEXP7,FaPG2,FaPLA,and Faβ-Gal4 as potential softening activators expressed before harvest to determine fruit with more softened texture and shorter shelf life,and that extremely high expression levels of FaCEL1-1 and FaCEL1-3 during ripening might be accelerators to intensify this situation.Additionally,both the enzyme activities of FaCEL and the expression pattern of FaCEL1-3 showed a significantly negative correlation with fruit firmness after harvest,suggesting that FaCEL1-3 might play a key role in promoting strawberry fruit softening not only during ripening but also postharvest.These results showed that the difference in fruit firmness and shelf life among cultivated strawberries was controlled by the temporal expression pattern of a legion of cell wall-associated genes during ripening and postharvest.
基金sponsored by the National Natural Science Foundation of China (Nos.21677156,41201498,21107125,51290282,51608515)the National Water Pollution Control and Treatment Science and Technology Major Project (No.2015ZX07205-003)the special fund from the State Key Joint Laboratory of Environment Simulation and Pollution Control (Research Center for Eco-environmental Sciences,Chinese Academy of Sciences) (No.16Z02ESPCR)
文摘Organic matter(OM) is an important component of sediment. Bioturbation/bioirrigation can remobilize OM and heavy metals that were previously buried in the sediment. The remobilization of buried organic matter, thallium(Tl), cadmium(Cd), copper(Cu) and zinc(Zn) from sediment was studied in a laboratory experiment with three organisms: tubificid,chironomid larvae and loach. Results showed that bioturbation/bioirrigation promoted the release of dissolved organic matter(DOM) and dissolved Tl, Cd, Cu and Zn, but only dissolved Zn concentrations decreased with exposure time in overlying water. The presence of organisms altered the compositions of DOM released from sediment,considerably increasing the percentage of fulvic acid-like materials(FA) and humic acidlike materials(HA). In addition, bioturbation/bioirrigation accelerated the growth and reproduction of bacteria to enhance the proportion of soluble microbial byproduct-like materials(SMP). The DOM was divided into five regions in the three-dimensional excitation emission matrix(3 D-EEM), and each part had different correlation with the dissolved heavy metal concentrations. Dissolved Cu had the best correlation with each of the DOM compositions, indicating that Cu in the sediment was in the organic-bound form.Furthermore, the organism type and heavy metal characteristics both played a role in influencing the remobilization of heavy metal.
基金supported by the National Natural Science Foundation of China (Nos. 21107125, 21577160, 51221892, 51290282 and 41201498)
文摘The heterogeneous Fenton reaction can generate highly reactive hydroxyl radicals(·OH)from reactions between recyclable solid catalysts and H2O2 at acidic or even circumneutral pH.Hence,it can effectively oxidize refractory organics in water or soils and has become a promising environmentally friendly treatment technology.Due to the complex reaction system,the mechanism behind heterogeneous Fenton reactions remains unresolved but fascinating,and is crucial for understanding Fenton chemistry and the development and application of efficient heterogeneous Fenton technologies.Iron-based materials usually possess high catalytic activity,low cost,negligible toxicity and easy recovery,and are a superior type of heterogeneous Fenton catalysts.Therefore,this article reviews the fundamental but important interfacial mechanisms of heterogeneous Fenton reactions catalyzed by iron-based materials..OH,hydroperoxyl radicals/superoxide anions(HO2./O2^-.)and high-valent iron are the three main types of reactive oxygen species(ROS),with different oxidation reactivity and selectivity.Based on the mechanisms of ROS generation,the interfacial mechanisms of heterogeneous Fenton systems can be classified as the homogeneous Fenton mechanism induced by surface-leached iron,the heterogeneous catalysis mechanism,and the heterogeneous reaction-induced homogeneous mechanism.Different heterogeneous Fenton systems catalyzed by characteristic iron-based materials are comprehensively reviewed.Finally,related future research directions are also suggested.
基金supported by the New Century Excellent Talents in University from the Ministry of Education of China (NCET-11-0537)the Fundamental Research Funds for the Central Universities (12lgpy52)
文摘Biogenic particles discharged by wastewater treatment plants play important roles in receiving water because of the large specific surface area and good mobility of the particles. In this study, the changes induced in biogenic particles by natural sunlight were investigated to understand the phototransformation of the particles in the receiving waters. The results showed that photoexposure resulted in significant decreases in the sizes of the biogenic particles but that photoexposure did not impact the zeta potentials. In addition, the photodissolution of biogenic particulate organic matter(POM) led to the generation of biogenic dissolved organic matter(DOM). Characterization using excitation–emission matrix(EEM) spectroscopy showed that photoexposure changed both the shapes and the intensities of the EEM spectra of the biogenic POM; the regions of the T1 and T2 peaks were susceptible to photoexposure. Modeling by parallel factor analysis(PARAFAC) decomposed the EEMs of the biogenic POM into four valid components, i.e., terrestrial or microbial humic-like substances, tryptophan-like proteins, tyrosinelike proteins and hydrophobic proteins. The humic-like substances in the biogenic POM from the effluents were subject to lower decreases(17.0 % and 11.6 %).Throughout the entire incubation time, the PARAFAC components in the biogenic DOM were dominated by elimination mechanisms rather than by production through photodissolution of the biogenic POM.
基金supported by the Key Project of the Natural Science Foundation of Tianjin(20JCZDJC00350)the National Natural Science Foundation of China(51803108)Tianjin Research Innovation Project for Postgraduate Students(2020YJSB066)。
文摘利用光热材料吸收太阳能产生局域化的热效应实现太阳能蒸汽转化是一项环境友好型海水淡化和废水净化的技术.本文受自然界中叶片蒸腾作用的启发,设计了一种新型仿生叶片结构,以实现高效的太阳能蒸汽转化.这种新型仿生叶片结构由三个部分组成:(1)亲水性聚乙烯醇(PVA)海绵层(相当于叶子中的叶肉组织层),具有高孔隙率和低导热性;(2)聚吡咯(PPy)层(相当于叶子中的叶绿素层)负载于PVA海绵表面以吸收太阳能;(3)微/纳米尺度的多孔疏水表面层(相当于叶片中的气孔层).其中,亲水海绵的PVA聚合物网络提供了高效水传输通道的同时实现了内部水蒸发焓的降低;PPy层吸收太阳能并转化为内能加热气液蒸汽界面的水,实现热局部化,大大减小了热损耗;疏水性多孔表面层的边缘效应,为水蒸气提供了高效逃逸通道.基于仿生叶片结构的太阳能蒸汽转化装置在一倍太阳光强(1 kW m^(−2))下蒸发率高达3.09 kg m^(−2) h^(−1).在室外自然光照条件下成功地对盐水和海水进行了脱盐和净化,平均净水速率为1.4 L m^(−2) h^(−1).这种基于仿生叶片结构的太阳能蒸汽转化装置提供了一种极具潜力的高效水净化方法,有助于利用可持续能源缓解全球水危机.
基金supported by the National Natural Science Foundation of China(Nos.51025830,41201498 and 51178360)
文摘Fenton oxidation is generally inhibited in the presence of a high concentration of chloride ions.This study investigated the feasibility of using benzoquinone(BQ) and hydroxylamine hydrochloride(HA) as Fenton enhancers for the removal of glycerin from saline water under ambient temperature by accelerating the ferric system.It was found that organics removal was not obviously affected by chloride ions of low concentration(less than 0.1 mol/L),while the mineralization rate was strongly inhibited in the presence of a large amount of chloride ions.In addition,ferric hydrolysis-precipitation was significantly alleviated in the presence of HA and BQ,and HA was more effective in reducing ferric ions into ferrous ions than HA,while the H_2O_2 decomposition rate was higher in the BQ-Fenton system.Electron spin resonance analysis revealed that OH production was reduced in high salinity conditions,while it was enhanced after the addition of HA and BQ(especially HA).This study provided a possible solution to control and alleviate the inhibitory effect of chloride ions on the Fenton process for organics removal.
基金supported by the National Natural Science Foundation of China(Nos.21107125,21577160,51290282,51221892)the National Basic Research Program(973s)of China(No.2011CB933704)the Hjalmar Lundbom Research Center at Lulea niversity of Technology
文摘The effect of phosphate on adsorption and oxidation of catechol, 1,2-dihydroxybenzene,in a heterogeneous Fenton system was investigated. In situ attenuated total reflectance infrared spectroscopy(ATR-FTIR) was used to monitor the surface speciation at the nano-Fe_3O_4 catalyst surface. The presence of phosphate decreased the removal rate of catechol and the abatement of dissolved organic compounds, as well as the decomposition of H2O2. This effect of phosphate was mainly due to its strong reaction with surface sites on the iron oxide catalyst. At neutral and acid pH, phosphate could displace the adsorbed catechol from the surface of catalyst and also could compete for surface sites with H2O2. In situ IR spectra indicated the formation of iron phosphate precipitation at the catalyst surface. The iron phosphate surface species may affect the amount of iron atoms taking part in the catalytic decomposition of H2O2 and formation of hydroxyl radicals,and inhibit the catalytic ability of Fe3O4 catalyst. Therefore, phosphate ions worked as stabilizer and inhibitor in a heterogeneous Fenton reaction at the same time, in effect leading to an increase in oxidation efficiency in this study. However, before use of phosphate as pH buffer or H2O2 stabilizer in a heterogeneous Fenton system, the possible inhibitory effect of phosphate on the actual removal of organic pollutants should be fully considered.
基金supported by the National Natural Science Foundation of China(No.51478487)
文摘Effluent organic matter(Ef OM) from municipal wastewater treatment plants potentially has a detrimental effect on both aquatic organisms and humans.This study evaluated the removal and transformation of chromophoric dissolved organic matter(CDOM) and fluorescent dissolved organic matter(FDOM) in a full-scale wastewater treatment plant under different seasons.The results showed that bio-treatment was found to be more efficient in removing bulk DOM(in term of dissolved organic carbon,DOC) than CDOM and FDOM,which was contrary to the disinfection process.CDOM and FDOM were selectively removed at various stages during the treatment.Typically,the low molecular weight fractions of CDOM and protein-like FDOM were more efficiently removed during bio-treatment process,whereas the humic-like FDOM exhibited comparable decreases in both bio-treatment and disinfection processes.Overall,the performance of the WWTP was weak in terms of CDOM and FDOM removal,resulting in enrichment of CDOM and FDOM in effluent.Moreover,the total removal of the bulk DOM(P 〈 0.05) and the protein-like FDOM(P 〈 0.05) displayed a significant seasonal variation,with higher removal efficiencies in summer,whereas removal of CDOM and the humic-like FDOM showed little differences between summer and winter.In all,the results provide useful information for understanding the fate and transformation of DOM,illustrating that sub-fractions of DOM could be selectively removed depending on treatment processes and seasonality.
文摘Single wall carbon nanotube(SWCNT)additives were formulated into(im-Si-graphite composite electrodes and tested in both half cells and full cells with high nickel cathodes.The critical role of small amount of SWCNT addition(0.2 wt%)was found for significantly improving delithiation capacity,first cycle coulombic efficiency(FCE),and capacity retention.Particularly,Si(10 wt%)-graphite electrode exhibits 560 mAh/g delithiation capacity and 92%FCE at 0.2 C during the first chargedischarge cycle,and 91%capacity retention after 50 cycles(0.5 C)in a half cell.Scanning electron microscope(SEM)was used to illustrate the electrode morphology,compositions and promoting function of the SWCNT additives.In addition,full cells assembled with high nickel-NCM811 cathodes and fim-Si-graphite composite anodes were evaluated for the consistence between half and full cell performance,and the consideration for potential commercial application.Finally,criteria to assess Si-containing anodes are proposed and discussed from an industrial perspective.