Numerical Prediction and Analysis of Hydraulic Characteristics of Waterfall Scene for Application to Design Control

The hydraulic characteristics of artificial waterfalls constitute an important research area for both academia and industry. As yet, the hydraulic parameters used in the hydraulic design of landscape waterfalls are not well understood for use in qualitative and quantitative analyses. Due to their complicated shapes and appearances, it remains a challenging problem to design or simulate realistic waterfall scenes. In this paper, we present a hybrid model and an efficient numerical method for simulating the hydraulic characteristics of waterfalls and investigate the critical flow in waterfall design and the adherent flow phenomenon. We fit empirical equations to predict the size and shape of different waterfall flow patterns to provide a basis for the design and construction of waterscapes in the real world. We then apply our work to an artificial waterfall in Kraal Jiang River and the results demonstrate that our model achieves desirable control qualities and good agreement in a real-world application. © 2017, Tianjin University and Springer-Verlag Berlin Heidelberg.

Comprehensive profiling of CTP-binding proteins using a biotinylated CTP affinity probe

Recent studies have shown that CTP may act as a ligand to regulate the activity of its target proteins in many biological processes.However,proteome-wide identification of CTP-binding proteins remains challenging.Here,we employed a biotinylated CTP affinity probe coupled with stable isotope labeling by amino acids in cell culture(SILAC)-based quantitative proteomics approach to capture,identify and quantify CTP-binding proteins in human cells.By performing two types of competitive SILAC experiments with high vs.low concentrations of CTP probe(100 vs.10µmol/L)or with CTP probe in the presence of free CTP,we identified 90 potential CTP-binding proteins which are involved in a variety of biological processes,including protein folding,nucleotide binding and cell-cell adhesion.Together,we developed a chemical proteomic method for uncovering the CTP-binding proteins in human cells,which could be widely applicable for profiling CTP-binding proteins in other biological samples.