An antigen self-assembled and dendritic cell-targeted nanovaccine for enhanced immunity against cancer

The rise of nanotechnology has opened new horizons for cancer immunotherapy.However,most nano vaccines fabricated with nanomaterials suffer from carrier-related concerns,including low drug loading capacity,unpredictable metabolism,and potential systemic toxicity,which bring obstacles for their clinical translation.Herein,we developed an antigen self-assembled nanovaccine,which was resulted from a simple acryloyl modification of the antigen to induce self-assembly.Furthermore,a dendritic cell targeting head mannose monomer and a mevalonate pathway inhibitor zoledronic acid(Zol)were integrated or absorbed onto the nanoparticles(denoted as MEAO-Z)to intensify the immune response.The synthesized nano vaccine with a diameter of around 70 nm showed successful lymph node transportation,high dendritic cell internalization,promoted costimulatory molecule expression,and preferable antigen cross-presentation.In virtue of the above superiorities,MEAO-Z induced remarkably higher titers of serum antibody,stronger cytotoxic T lymphocyte immune responses and IFN-γsecretion than free antigen and adjuvants.In vivo,MEAO-Z significantly suppressed EG7-OVA tumor groth and prolonged the survival time of tumor-bearing mice.These results indicated the translation promise of our self-assembled nano vaccine for immune potentiation and cancer immunotherapy.

Blended Teaching Mode Based on“Cloud Class+PAD Class”in the Course of Computer Graphics and Image Design

As a teaching tool,Cloud class is specially developed for teaching and learning in mobile circumstance,and the PAD(Presentation Assimilation Discussion)class acts as a creative teaching mode.The combination of Cloud class and PAD class helps build online-offline blended teaching model.In this paper,a Cloud+PAD class mode is proposed.An experiment of teaching is implemented and the practical effectiveness is evaluated and verified.The experimental results show,compared with the conventional teaching method,the proposed teaching mode is capable of creating more high-level,innovative and complex teaching content,and thereby noticeably enhancing students’learner autonomy and motivating them to develop creativity.