Inducible expression of endomorphins in murine dendritic cells

Bone marrow precursor cells were extracted from C57BL/6J mice aged 7-8 weeks, and dendritic cells were purified using anti-CD1 lc （a specific marker for dendritic cells） antibody-coated magnetic beads. Immunofluorescence staining revealed that the expression levels of endomorphin-1 and endomorphin-2 were upregulated in dendritic cells activated by lipopolysaccharide. An enzyme Jmmunoassay showed that lipopolysaccharide and other Toll-like receptor ligands promoted the secretion of endomorphin-1 and endomorphin-2 from activated dendritic cells. [3H]-thymidine incorporation demonstrated that endomorphin-1 and endomorphin-2 both inhibited the proliferation of T lymphocyte induced by activated dendritic cells. Furthermore, this immunosuppressive effect was blocked by CTOP, a specific antagonist of IJ-opioid receptors. Our experimental findings indicate that activated dendritic cells can induce the expression and secretion of endomorphins, and that endomorphins suppress T lymphocyte proliferation through activation of iJ-opioid receptors.

Chinese consensus guidelines for therapeutic drug monitoring of polymyxin B,endorsed by the Infection and Chemotherapy Committee of the Shanghai Medical Association and the Therapeutic Drug Monitoring Committee of the Chinese Pharmacological Society

Polymyxin B,which is a last-line antibiotic for extensively drug-resistant Gram-negative bacterial infections,became available in China in Dec.2017.As dose adjustments are based solely on clinical experience of risk toxicity,treatment failure,and emergence of resistance,there is an urgent clinical need to perform therapeutic drug monitoring(TDM)to optimize the use of polymyxin B.It is thus necessary to standardize operating procedures to ensure the accuracy of TDM and provide evidence for their rational use.We report a consensus on TDM guidelines for polymyxin B,as endorsed by the Infection and Chemotherapy Committee of the Shanghai Medical Association and the Therapeutic Drug Monitoring Committee of the Chinese Pharmacological Society.The consensus panel was composed of clinicians,pharmacists,and microbiologists from different provinces in China and Australia who made recommendations regarding target concentrations,sample collection,reporting,and explanation of TDM results.The guidelines provide the first-ever consensus on conducting TDM of polymyxin B,and are intended to guide optimal clinical use.

Investigation on surface roughness,residual stress and fatigue property of milling in-situ TiB_2/7050Al metal matrix composites

For higher efficiency and precision manufacturing,more and more attentions are focused on the surface roughness and residual stress of machined parts to obtain a good fatigue life.At present,the in-situ TiB_2/7050 Al metal matrix composites are widely researched due to its attractive properties such as low density,good wear resistance and improved strength.It is of great significance to investigate the machined surface roughness,residual stress and fatigue life for higher efficiency and precision manufacturing of this new kind material.In this study,the surface roughness including two-dimensional and three-dimensional roughness,residual stress and fatigue life of milling in-situ TiB_2/7050 Al metal matrix composites were analyzed.It was found from comparative investigation that the three-dimensional surface roughness would be more appropriate to represent the machined surface profile of milling particle reinforced metal matrix composites.The cutting temperature played a great role on the residual stress.However,the effect of increasing cutting force could slow down the transformation from compressive stress to tensile stress under 270°C.An exponential relationship between three-dimensional roughness and fatigue life was established and the main fracture mechanism was brittle fracture with observation of obvious shellfish veins,river pattern veins and wave shaped veins in fracture surface.

Ethylene†and fruit softening

This review is concerned with the mechanisms controlling fruit softening.Master genetic regulators switch on the ripening programme and the regulatory pathway branches downstream,with separate controls for distinct quality attributes such as colour,flavour,texture,and aroma.Ethylene plays a critical role as a ripening hormone and is implicated in controlling different facets of ripening,including texture change,acting through a range of transcriptional regulators,and this signalling can be blocked using 1-methylcyclopropene.A battery of at least seven cell-wall-modifying enzymes,most of which are synthesized de novo during ripening,cause major alterations in the structure and composition of the cell wall components and contribute to the softening process.Significant differences between fruits may be related to the precise structure and composition of their cell walls and the enzymes recruited to the ripening programme during evolution.Attempts to slow texture change and reduce fruit spoilage by delaying the entire ripening process can often affect negatively other aspects of quality,and low temperatures,in particular,can have deleterious effects on texture change.Gene silencing has been used to probe the function of individual genes involved in different aspects of ripening,including colour,flavour,ethylene synthesis,and particularly texture change.The picture that emerges is that softening is a multi-genic trait,with some genes making a more important contribution than others.In future,it may be possible to control texture genetically to produce fruits more suitable for our needs.

Analytical model of cutting force in axial ultrasonic vibration-assisted milling in-situ TiB_(2)/7050Al PRMMCs

Ultrasonic vibration-assisted milling has been widely applied in machining the difficultto-cut materials owing to the remarkable improvements in reducing the cutting force.However,analytical models to reveal the mechanism and predict the cutting force of ultrasonic vibrationassisted milling metal matrix composites are still needed to be developed.In this paper,an analytical model of cutting force was established for ultrasonic vibration-assisted milling in-situ TiB_(2)/7050 Al metal matrix composites.During modeling,change of motion of the cutting tool,contact of toolchip-workpiece and acceleration of the chip caused by ultrasonic vibration was considered based on equivalent oblique cutting model.Meanwhile,material properties,tool geometry,cutting parameters and vibration parameters were taken into consideration.Furthermore,the developed analytical force model was validated with and without ultrasonic vibration milling experiments on in-situ TiB_(2)/7050 Al metal matrix composites.The predicted cutting forces show to be consistent well with the measured cutting forces.Besides,the relative error of instantaneous maximum forces between the predicted and measured data is from 0.4%to 15.1%.The analytical model is significant for cutting force prediction not only in ultrasonic-vibration assisted milling but also in conventional milling in-situ TiB_(2)/7050 Al metal matrix composites,which was proved with general applicability.

Mechanism regulating the inhibition of lung cancer A549 cell proliferation and structural analysis of the polysaccharide Lycium barbarum

The water-soluble polysaccharide LBP-1 was isolated and characterized from Lycium barbarum L.LBP-1 was mainly composed of arabinose,galactose,glucose,xylose,mannose at a molar ratio of 37.53:28.08:14.72:7.83:4.50,respectively.Based on nuclear magnetic resonance(NMR)and methylation analysis,the backbone of LBP-1 was speculated in theα-L-Ara(1→[5-α-L-Ara(1→3)-β-D-Galp-(1→]n→4)-α-D-Galp-(1[→5-α-L-Ara(1]n[→6)-β-D-Galp-(1→4)-β-D-Galp-(1→]n,and the side chains of LBP-1 were in theα-L-Ara(1→3)-β-D-Galp-(1→6 position.These results showed that LBP-1 inhibited the growth of cancer A549 cells through cell cycle arrest and apoptosis,with an IC50 value of 42.5μg/mL.In addition,LBP-1 altered the expression of Cyclin D1,Cyclin D3,and CDK 2,thus blocking the cell cycle in G0/G1 phase,reducing cell migration,and regulating the PI3K/Akt/mTOR signaling pathway to induce apoptosis.

The structure of WbnH in a near active state

Dear Editor, Gram-negative bacteria possess a complicated membrane system that plays an essential role in interactions between bacteria and the envJronment （Reeves and Wang, 2002）. The inner leaflet of the membrane is composed of various glycerophospholipids, and the outer leaflet consists primarily of lipopolysaccharide （LPS） molecules.

Analysis of stability and robust stability for stochastic hybrid systems with impulsive effects

In this article,the problems of stability and robust stability analysis are investigated for a class of Markovian switching stochastic systems,which has impulses at switching instants.The switching parameters considered form a continuous-time discrete-state homogeneous Markov process.Multiple Lyapunov techniques are used to derive sufficient conditions for stability in probability of the overall system.The conditions are in linear matrix inequalities form,and can be used to solve stabilization synthesis problems.The results are extended to the design of a robust-stabilized state-feedback controller as well.A numerical example shows the effectiveness of the proposed approach.