Objective： Studies have shown that TNF- α secreted by tumor ceils and macrophages infiltrated into the tumor microenvironment might promote the metastasis of a variety of malignant cancers, including breast cancer. ...Objective： Studies have shown that TNF- α secreted by tumor ceils and macrophages infiltrated into the tumor microenvironment might promote the metastasis of a variety of malignant cancers, including breast cancer. The present study was designed to detect global metastasis-related gene expression changes of MCF-7 cells treated by low dose TNF-α and to further explore the mechanisms by which TNF- α contributes to metastasis. Methods： MCF-7 cells were cultured and treated with low dose TNF-α （20 ng/ml）, cDNA array analysis was applied to detect the metastasis related gene expressions. Results： A total of 36 gene expressions were significantly regulated by TNF- α. Functional analysis indicates that the altered genes belong to different functional group. Most of the genes changed may promote the metastasis of MCF-7 cells while the others may inhibit metastasis. The changes observed in gene expression following TNF-α were somewhat time dependent. Conclusion： TNF-α can enhance the invasive ability of MCF-7 cells, partly by regulating a series of metastasis related genes, and these genes may take part in every step of metastasis. Some of the genes deserve further study.展开更多
基金supported by the National Nature Science Foundation of China(NO.30772474).
文摘Objective： Studies have shown that TNF- α secreted by tumor ceils and macrophages infiltrated into the tumor microenvironment might promote the metastasis of a variety of malignant cancers, including breast cancer. The present study was designed to detect global metastasis-related gene expression changes of MCF-7 cells treated by low dose TNF-α and to further explore the mechanisms by which TNF- α contributes to metastasis. Methods： MCF-7 cells were cultured and treated with low dose TNF-α （20 ng/ml）, cDNA array analysis was applied to detect the metastasis related gene expressions. Results： A total of 36 gene expressions were significantly regulated by TNF- α. Functional analysis indicates that the altered genes belong to different functional group. Most of the genes changed may promote the metastasis of MCF-7 cells while the others may inhibit metastasis. The changes observed in gene expression following TNF-α were somewhat time dependent. Conclusion： TNF-α can enhance the invasive ability of MCF-7 cells, partly by regulating a series of metastasis related genes, and these genes may take part in every step of metastasis. Some of the genes deserve further study.
