LncRNA DPP10-AS1 promotes malignant processes through epigenetically activating its cognate gene DPP10 and predicts poor prognosis in lung cancer patients

Objective:The purpose of this study was to explore the function and gene expression regulation of the newly identified lnc RNA DPP10-AS1 in lung cancer,and its potential value as a prognostic biomarker.Methods:q RT-PCR and Western blot were conducted to detect the expression of DDP10-AS1 and DPP10 in lung cancer cell lines and tissues.The effects of DDP10-AS1 on DPP10 expression,cell growth,invasion,apoptosis,and in vivo tumor growth were investigated in lung cancer cells by Western blot,rescue experiments,colony formation,flow cytometry,and xenograft animal experiments.Results:The novel antisense lnc RNA DPP10-AS1 was found to be highly expressed in cancer tissues(P<0.0001),and its upregulation predicted poor prognosis in patients with lung cancer(P=0.0025).Notably,DPP10-AS1 promoted lung cancer cell growth,colony formation,and cell cycle progression,and repressed apoptosis in lung cancer cells by upregulating DPP10 expression.Additionally,DPP10-AS1 facilitated lung tumor growth via upregulation of DPP10 protein in a xenograft mouse model.Importantly,DPP10-AS1 positively regulated DPP10 gene expression,and both were coordinately upregulated in lung cancer tissues.Mechanically,DPP10-AS1 was found to associate with DPP10 m RNA but did not enhance DPP10 m RNA stability.Hypomethylation of DPP10-AS1 and DPP10 contributed to their coordinate upregulation in lung cancer.Conclusions:These findings indicated that the upregulation of the antisense lnc RNA DPP10-AS1 promotes lung cancer malignant processes and facilitates tumorigenesis by epigenetically regulating its cognate sense gene DPP10.DPP10-AS1 may serve as a candidate prognostic biomarker and a potential therapeutic target in lung cancer.

Clinical characteristics and coronary features of coronary ectasia and aneurysm in China

Objective: To describe the clinical characteristics and coronary features of patients with coronary ectasia and aneurysm (CEA) in China. Methods: This was a single center, retrospective clinical study. Patients with CEA who diagnosed by coronary artery angiography (CAG) from April 2003 to October 2009, were enrolled. All patients were followed up by the call or clinic visit. The primary outcome was all-cause mortality or cardiovascular readmission. Results: A total of 39 patients (27 male, mean age 59.5 ± 12.6 years) diagnosed with CEA, with a prevalence of 0.6% (a total of 6,130 patients underwent CAG during the same period). Thirty-six patients constituted the study population, due to 3 patients lost of follow-up. The median follow-up was 27 months (1 - 76). The single vessel was involved in 11 patients (31%), two vessels were involved in 14 patients (38%) and three or more vessels were involved in 11 patients (31%). The most common involved vessel was left anterior descending branch (n = 27), followed by right coronary artery (n = 23), left circumflex branch (n = 20) and left main branch (n = 9). There was no death during the follow-up. The primary outcome was observed in 8 (22%) patients. The use of clopidogrel and statins were associated with lower rate of cardiovascular readmission. Conclusion The incidence of CEA was low among patients who referred for CAG in China. The majority of patients had the involvement of multiple vessels, and left anterior descending branch was the most common involved vessel. The CEA patients may benefit from clopidogrel and statins.

浙江野生种子植物的分布格局与区系分区

植物的分布格局和区系分区研究可以为植物资源开发、物种引种、植物多样性保护以及农、林、牧的远景规划提供依据。本文基于最近出版的《浙江植物志(新编)》记载的相关数据,分析了浙江省野生种子植物的分布格局,探讨了植物区系的分区。结果显示:浙江省有种子植物212科1,469属4,430种(不计种下分类群),其中自然分布的野生种子植物有190科1,085属3,344种,包含中国特有属45个,中国特有种1,405个。属级水平上,广布属有548个,占野生种子植物总属数的50.5%;各区域中属数最多的是洞宫山区(851属),其次是浙东南沿海区(822属)和天目山区(818属),最少的是浙北平原区(仅有411属)。种级水平上,种数最多的是洞宫山区(2,304种),其次是天目山区和仙霞岭区(均超过2,000种),最少的是浙北平原区(仅有721种)。按照浙江种子植物分布的区域数目分为广布种(904个)、常见种(846个)、局限种(1,000个)、区域独有种(594个);常见种和局限种共计1,846种,可归纳为以下6个基本分布式样,浙北部分布(171种)、浙南部分布(280种)、浙西部分布(109种)、浙东部分布(93种)、浙西至浙东南分布(98种)、浙东北至浙西南分布(37种),其余为丘陵山地散布和间断分布。根据浙江野生种子植物属和种的分布,结合前人的区系分区研究成果,将浙江植物区系划分成2个地区(华东地区和岭南山地地区)3个亚地区(黄淮平原亚地区、浙南山地亚地区和闽北山地亚地区)5个小区(浙北平原小区、浙江中北部山地丘陵小区、浙东北沿海小区、浙江中南部山地丘陵小区、浙东南沿海小区)。

Artificial intelligence biosensors for continuous glucose monitoring

Artificial intelligence(AI)algorithms in combination with continuous monitoring technologies have the potential to revolutionize chronic disease management.The recent innovations in both continuous glucose monitoring(CGM)and the closed-loop highlight the far-reaching potential of AI biosensors for individual healthcare.This review summarizes some of the most advanced progress made in CGM biosensing.We will focus on three main applications of AI algorithms in diabetes management:closed-loop control algorithms,glucose predictions,and calibrations.The challenges and opportunities of AI technologies for CGM in individualized and proactive medicine will also be discussed.

濒危植物百山祖冷杉种子发育特征及其胚培养

百山祖冷杉(Abies beshanzuensis)种子萌发率低,发育特性尚不明确,极大地限制了种群在原生境的自然更新。该研究以百山祖冷杉不同发育时期的雌球果为材料,通过研究球果中种子的胚与胚乳数量、重量和显微结构特性,及测定种子发育关键时期胚乳的初生代谢物,探究种子发育特征及影响种子发育的主要因素。结果表明, 7月是百山祖冷杉种子发育的关键时期,此期间胚乳重量快速增加,胚乳中碳水化合物代谢活动旺盛。解剖学和形态学分析显示,7月下旬球果中大量种子发育异常。进一步对发育关键时期胚乳中初生代谢物进行分析表明,海藻糖-6-磷酸含量显著降低。推测在种子发育的关键时期胚乳中海藻糖-6-磷酸降低可能是种子发育异常的重要原因之一。基于种子发育特征,该研究建立了百山祖冷杉未成熟胚培养技术体系,并成功获得试管苗。

浙江种子植物物种编目

生物多样性的编目和分类以及生物多样性监测是全球生物多样性研究的两个核心内容。物种编目是了解物种多样性的基础,只有掌握物种分布格局及物种与环境的关系,才能为物种监测和科学管理提供依据。作为浙江种子植物研究的“家底”,本文在《浙江植物志(新编)》编研的基础上,系统整理了浙江种子植物的物种名录。结果显示,浙江共有种子植物212科1,469属4,430种,其中野生植物有190科1,085属3,347种。所含种数多于100种的科有禾本科(285种)、莎草科(216种)、菊科(186种)、蔷薇科(153种)、兰科(126种)、蝶形花科(109种)和唇形科(108种);含20种以上的属有15属,包括薹草属(Carex,126种)、刚竹属(Phyllostachys,44种)、悬钩子属(Rubus,44种)、冬青属(Ilex,35种)、蓼属(Polygonum,34种)、珍珠菜属(Lysimachia,32种)、铁线莲属(Clematis,31种)、景天属(Sedum,28种)、槭属(Acer,26种)、荚蒾属(Viburnum,26种)、飘拂草属(Fimbristylis,26种)、蒿属(Artemisia,25种)、堇菜属(Viola,22种)、葡萄属(Vitis,21种)和山矾属(Symplocos,21种)。其区系特点主要反映在:物种丰富,其科属组成多样;保存了较多古老孑遗植物;地理成分多样,联系广泛,由热带向温带过渡;中国特有科2科、中国特有属45属、浙江(准)特有种近500种(含亚种和变种),珍稀濒危植物丰富,96种为国家重点保护植物;外来植物多,入侵风险大,有些种已形成明显的危害。

An Optical Fiber Hydrophone Using Equivalent Phase Shift Fiber Bragg Grating for Underwater Acoustic Measurement

一个光纤维水中听音器基于有温度赔偿包裹的相等的阶段移动纤维布拉格栅栏(EPS-FBG ) 提供敏感的改进在在水下在宽频率范围的声学的测量，从 2.5 kHz 到 12 kHz。声学的压力是进一个圆金属磁盘的有弹性的颤动的 transduced，导致从纤维布拉格栅栏(FBG ) 的回来的反映的轻波浪的紧张调整。实验证明包装 EPS-FBG 水中听音器有大约 500 Pa/$\sqrt 的一个最小的可检测的声学的压力 { Hz }$\sqrt { Hz } 在 5 kHz 并且与一个常规 apodized FBG 水中听音器相比完成声学的压力敏感的大约 18-dB 改进。

The anomalous Hall effect in epitaxial Fe(110) films grown on Ga As(110)

The anomalous Hall effect in epitaxial Fe(110)films grown on Ga As(110) is investigated as a function of both film thickness and temperature. The Berry curvatureinduced intrinsic contribution of 996 X 1cm 1is determined experimentally for the first time. Together with 821 X 1cm 1in Fe(111) and 1100 X 1cm 1in Fe(001)obtained earlier, we show unambiguously the anisotropy of the Berry curvature contribution to the anomalous Hall effect in single-crystal Fe.

A targeted covalent inhibitor of p97 with proteome-wide selectivity

A resurging interest in targeted covalent inhibitors(TCIs)focus on compounds capable of irreversibly reacting with nucleophilic amino acids in a druggable target.p97 is an emerging protein target for cancer therapy,viral infections and neurodegenerative diseases.Extensive efforts were devoted to the development of p97 inhibitors.The most promising inhibitor of p97 was in phase 1 clinical trials,but failed due to the off-target-induced toxicity,suggesting the selective inhibitors of p97 are highly needed.We report herein a new type of TCIs(i.e.,FL-18)that showed proteome-wide selectivity towards p97.Equipped with a Michael acceptor and a basic imidazole,FL-18 showed potent inhibition towards U87 MG tumor cells,and in proteome-wide profiling,selectively modified endogenous p97 as confirmed by in situ fluorescence scanning,label-free quantitative proteomics and functional validations.FL-18 selectively modified cysteine residues located within the D2 ATP site of p97.This covalent labeling of cysteine residue in p97 was verified by LC-MS/MS-based site-mapping and site-directed mutagenesis.Further structure-activity relationship(SAR)studies with FL-18 analogs were established.Collectively,FL-18 is the first known small-molecule TCI capable of covalent engagement of p97 with proteome-wide selectivity,thus providing a promising scaffold for cancer therapy.

Novel phenyl-urea derivatives as dual-target ligands that can activate both GK and PPARγ

A series of novel phenyl-urea derivatives which can simultaneously activate gluco kinase(GK)and peroxisome proliferator-activated receptorγ(PPARγ)was designed and prepared,and their activation of GK and PPARγ was evaluated.The structure--activity relationships of these compounds are also described.Three compounds showed potent ability to activate both GK and PPARγ.The possible binding mode of one of these compounds with GK and PPARγ were predicted by molecular docking simulation.

Generation and propagation characteristics of electromagnetic vortices in radio frequency

Electromagnetic vortices, which describe the orbital angular momentum(OAM) carrying waves with a helical phase front, have recently attracted much interest in a radio frequency domain due to their potential applications in many diverse areas. In an OAM-based scenario, the antenna for OAM mode multiplexing/demultiplexing plays an essential role in controlling the overall system performance. In this paper, we demonstrated theoretically and experimentally an easily realized OAM antenna based on the traveling-wave circular loop structure for efficiently multiplexing/demultiplexing multiple OAM modes; in addition, its general propagation characteristics including the polarization, divergence, and radiation pattern are mathematically analyzed. Schemes for antenna size reduction and various radiation pattern manipulations have also been discussed to realize a more flexible and compact system.

Derivation of Non-Integration Induced Pluripotent Stem Cells from Fibroblast of Severe Deafness Patients with GJB2 Mutation

In all the connexin-associated human diseases, deafness is one of the most important diseases with high frequency. The mu- tations of GJB2 （gap junction protein β2, also called connexin 26, Cx26） gene link with nonsyndromic or syndromic senso- rineural hearing loss and were shown to account for a large proportion of congenital deaf cases in many studied populations （del Castillo and del Castillo, 2011）. For example, the 235de1C mutation in GJB2 shows the frequency of approximately 1% and is the most frequent mutation in East Asian population （Yan et al., 2003）. Many efforts have been put to study the function of Gjb2 gene in both mouse model and human. In mouse, extensive deletion of Gjb2 causes embryo lethal due to the decreased transplacental glucose uptake, which was not found in human （Takata and Hirano, 1997; Gabriel et al., 1998）. In human, GJB2 deficiency is not able to cause embryo lethal （D＇Andrea et al., 2002）. However, the study of GJB2-associated hearing loss is hampered by many difficulties, such as unobtainable human cochlea and acoustic nerve tissues, and therefore the GJB2-associated hearing loss are underlying mechanisms of still remaining unclear.

Spurious-free dynamic range improvement in a photonic time-stretched analog-to-digital converter based on third-order predistortion

Spurious-free dynamic range(SFDR)limited by intermodulation distortions is a usually accepted measure for dynamic performance of a photonic time-stretched analog-to-digital converter(ADC).In this paper,SFDR improvement in a photonic time-stretched ADC based on third-order predistortion is proposed.The third-order predistortion is achieved optically within an integrated dual-parallel Mach–Zehnder modulator(DPMZM).The mechanism of SFDR improvement with third-order predistortion in the DPMZM is theoretically analyzed.Compared with a conventional scheme without predistortion,the experimental results show that the SFDR improvement of∼26 dB in the proposed scheme is proved.

A Compact PSOAM Antenna Based on Substrate Integrated Waveguide

With no dark zone in the propagation path and the same divergence angles,different plane spiral orbital angular momentum(PSOAM)beams can be superposed easily in the transverse plane.In this paper,a stacked resonator antenna based on substrate integrated waveguide is proposed,which is a compact planar waveguide structure.To obtain the structured EM wave which is superposed by the PSOAM waves(3;4;5;6)at 10 GHz,eight ring resonators are placed symmetrically on the two sides of the center plane and excited simultaneously.Simulation results,including distributions of the electric field,far-field results and purity analysis,are given,indicating that PSOAM beams can be generated with good performances.By controlling the initial phases of different PSOAM waves,beam steering can be realized with the same radiation patterns,which has great potentials in communication and radar detection.