Optimization of tracer coating parameters and their effects on the mechanical properties and quality of food-grade tracers for grain traceability

The purpose of this study was to optimize the coating process of food-grade tracers to manufacture tracers with good physical,mechanical and practical properties and an excellent appearance.The effects of the coating weight gain(1.00%-5.00%),coating solution spray rate(1.50-7.50 g/min)and tablet bed temperature(30℃-40℃)on the coating appearance quality,moisture absorption rate,friction coefficient,peak shear force,breaking rate,barcode recognition rate,transport wear rate and transport recognition rate were analysed using a Box-Behnken design(BBD)of response surface methodology(RSM).The experimental data were fitted to quadratic polynomial models by multiple regression analysis.The mathematical models of the barcode recognition rate,transport wear rate and transport recognition rate exhibited no statistically significant difference in these data.The optimum coating parameters were as follows:a 5.00%coating weight gain,spray rate of 5.47 g/min and tablet bed temperature of 35.42℃.Under the optimized conditions,the tracers had a good appearance(coating appearance quality),moisture resistance(moisture absorption rate),and frictional(friction coefficient),compression(peak shear force),and impact characteristics(breaking rate).

Quantitative determination of multi-class bioactive constituents for quality control of Yiqi Jiangzhi Granules

Objective:To establish a reliable and sensitive method for evaluating quality of Yiqi Jiangzhi Granules(YQJZG).Methods:Ultra performance liquid chromatography electrospray ionization tandem mass spectrometry(UPLC-ESI-MS/MS)was employed for simultaneous determination of eight marker components.Separation was performed on an AQUITY UPLC;HSS T3 column,the mobile phase consisted of acetonitrile as the organic phase and 0.1%(volume percentage)formic acid as the aqueous.Eight marker components,ginsenoside Rg1(GRg1),ginsenoside Re(GRe),ginsenoside Rb1(Gb1),typhaneoside(TEO),isorhamnetin-3-O-neohespeidoside(IN),hesperidin(HPD),aurantio-obtusin-6-O-β-D-glucoside(AG)and curcumin(CCM),were detected by multiple reaction monitoring(MRM)mode.The Chinese Pharmacopoeia(2020 edition)was regarded as the guidance document for this method validation.Results:The method showed good linearity(R^(2)≥0.9990).The relative standard deviation(RSD)values for the instrument precision,intermediate precision and repeatability were less than 2.91%,2.88%,and 3.54%,respectively.The average recovery varied from 91.08%to 103.89%,with RSD below 3.81%.Sample solutions were found to be stable within 24 h at 4℃(RSD<2.85%).Eight marker components were successfully determined from three batches of YQJZG.Conclusion:The proposed UPLC-ESI-MS/MS method was found to be simple,fast and sensitive,and can be used for the routine quality assessment of YQJZG.Simultaneously,this method may provide a new and powerful tool of quality control for other traditional Chinese medicine analogous formulae.