Circular RNA circFOXM1 triggers the tumorigenesis of non-small cell lung cancer through miR-132-3p/TMEM14A axis

Farlier studies indicated that circular RNAs(circRNAs)were found in various cancer clls,and circFOXM1 was reported to act as an oncogane in non-small cell lung cancer(NSCLC).However,the function of circFOXM1 in NSCLC remains undear.The epression lewels of genes were measured using quantative real-time polymerase chain reactions(qRT-PCR).Cell prolferation and apoptosis were determined by 3-(4,5 dimethylthiazol-2-yl)-25 dipbenyletrazolium bromide solution(MTT)and flow cytometry assay.The rdative protein expression was assed by westen blot Moreower,transwell assays were employed to examine ell migration and invasion.The targeted relationship was confirmed by dual-luciferase reporter assay.The expression of circFOXMI was up-regulated in NSCIC tissues and cell lines.The depletion of circFOXM1 decreased the prolferation,migration,invasion,and induced cell apoptosis of NSCLC cells.MicroRNA-132-3p(MiR-1323p)was idenified as a target of dircFOXMl.The expression level of miR-132-3p was decrased in NSCLC tssues and cell lines and inversely corrdated with circFOXM1 expression.Furthermore,the efects of drdOXMl down regulation on NSCLC cell progression were abolished by mR-1323p inhibitor.Transmembrane protein 14A(TMEM14A)was verifed as a target gene of miR-132-3p.The efects of circFOXM1 depletion on NSCLC cell proliferation,apoptosis,migration,and invasion were reversed by TMEMI4A overexpression.Our study demonstrated that knodkdown of circFOXM1 suppressed NSCLC progression through rqgulating miR-132-3p/TMEMI4A axis,sugesting the drFOXM/miR-132-3p/TMEM14A axis may serve as the novd target for NSCLC diagnosis and therapy.

Comparison and Correlation Study of Polar Biomarkers of CKD Patients in Saliva and Serum by UPLC-ESI-MS

There are abundant reports on the use of aromatic amino acids and creatinineas biomarkers in serum and urine forCKD(chronic kidney disease). However, investigationsinto these bio-species in bio-fluids, such as saliva and sweat, are rarely reported. Increasing interest in non-invasive methods for medical diagnosis advocates for the testing of these bio-fluidstoidentify potential biomarkers for prompt clinical andpreliminary screening using advanced analytical equipment. Unstimulated whole saliva samples were obtained from twenty-seven CKD patients and an equivalent number of healthy individuals. Saliva was assayed with ultra-performance liquid chromatography coupled with electro-spray ionization tandem mass spectrometry (UPLC-ESI-MS) in hydrophilic interaction chromatography mode. The data were analyzed using a student’s t test and receiver operatingcharacteristic (ROC) to evaluate the predictive power of several potential biomarkers (P<0.01) in saliva for preliminary CKD screening. Through testing of salivary samples between CKD patients and healthy individuals, we found three possible salivary biomarkers that demonstrated significant differences (P<0.01) from the nine reported species in serum and/or urine. The area under the curve (AUC) values for control vs CKDpatientsfor on L-phenylalanine, L- tryptophan, and creatinine were 0.863, 0.834, and 0.916, respectively. This is the first report to compare serum and urine biomarkers in saliva between CKD patients and healthy people. This study explores the potential of CKD diagnosis by saliva, and demonstrates a positive correlation between salivary and serum creatinine.

Two quasi-interfacial p-n junctions observed by a dual-irradiation system in perovskite solar cells

In general,perovskite solar cells(PSC)with a sensitized or thin-film architecture absorb light from a single-side illumination,and carrier separation and transport only take place inside the active layer of the perovskite film.Herein,we demonstrated a dualirradiation PSC system in which light passes through both the fluorinated tin oxide(FTO)side and the Au electrode side,resulting in much faster interfacial charge carrier extraction and transportation than that in a single-irradiation system,in which light passes through from either the FTO or semitransparent Au electrode side.This dual-irradiation PSC system with a configuration of FTO/Cl-TiO_(2)/Mp-TiO_(2)/mixed perovskite/spiro-OMeTAD/Au/ITO can form two quasi-interfacial p-n junctions,which occur separately at the interfaces of TiO_(2)/perovskite and perovskite/spiro-OMeTAD.When the PSC device was illuminated simultaneously from both the FTO and Au/ITO sides,the PSC achieved a total power conversion efficiency(PCE)as high as 20.1%under high light intensity(1.4 sun),which is higher than PCE(18.4%)of a single-irradiation system.The time of flight(TOF)photoconductivity,small perturbation transient photovoltaic(TPV),finite-difference time-domain(FDTD)optical simulations,and dual illumination-sidedependent impedance spectroscopy(ISD-IS)were used to authenticate the presence of two quasi-interfacial p-n junctions in the PSC,creating more charge carriers than only one quasi p-n junction,and thus leading to a fast recombination process.