Rice is one of the most important crops, providing staple food for about half population of the world. Drought stress affects plant growth and development seriously. This article reviewed the research progress of the ...Rice is one of the most important crops, providing staple food for about half population of the world. Drought stress affects plant growth and development seriously. This article reviewed the research progress of the physiological and molecular biology mechanism including osmotic adjustment, scavenging oxidative radicals, endogenous hormones, drought-resistance genes and epigenetic modification, it may be afford interrelated reference for increasing rice drought resistance and breeding drought resistance rice varieties.展开更多
HPLC analysis is important for determination of total level on DNA methylation in plants. It can be used to help characterise epigenetic changes during growth, development and stress. HPLC methods have been optimised ...HPLC analysis is important for determination of total level on DNA methylation in plants. It can be used to help characterise epigenetic changes during growth, development and stress. HPLC methods have been optimised for mammalian and microbial DNA, but not for plants. This article examines several important factors in the HPLC analysis of plant DNA methylation including extraction and purification of DNA and HPLC conditions choice by using leaves of rice seedling. The experimental results showed that RNA of nucleic acid was removed by using RNase A. This study also identified critical components of HPLC analysis. With the optimized method of HPLC conditions, the better result was achieved in the chromatogram of cytosine and 5-methylcytosine in genomic DNA acid hydrolysis. The study would offer a comprehensive guide for the stringent analysis of DNA methylation in plants.展开更多
文摘Rice is one of the most important crops, providing staple food for about half population of the world. Drought stress affects plant growth and development seriously. This article reviewed the research progress of the physiological and molecular biology mechanism including osmotic adjustment, scavenging oxidative radicals, endogenous hormones, drought-resistance genes and epigenetic modification, it may be afford interrelated reference for increasing rice drought resistance and breeding drought resistance rice varieties.
文摘HPLC analysis is important for determination of total level on DNA methylation in plants. It can be used to help characterise epigenetic changes during growth, development and stress. HPLC methods have been optimised for mammalian and microbial DNA, but not for plants. This article examines several important factors in the HPLC analysis of plant DNA methylation including extraction and purification of DNA and HPLC conditions choice by using leaves of rice seedling. The experimental results showed that RNA of nucleic acid was removed by using RNase A. This study also identified critical components of HPLC analysis. With the optimized method of HPLC conditions, the better result was achieved in the chromatogram of cytosine and 5-methylcytosine in genomic DNA acid hydrolysis. The study would offer a comprehensive guide for the stringent analysis of DNA methylation in plants.
