Satisfactory results cannot be obtained when three-dimensional (3D) targets with complex maneuvering characteristics are tracked by the commonly used two-dimensional coordinated turn (2DCT) model. To address the probl...Satisfactory results cannot be obtained when three-dimensional (3D) targets with complex maneuvering characteristics are tracked by the commonly used two-dimensional coordinated turn (2DCT) model. To address the problem of 3D target tracking with strong maneuverability, on the basis of the modified three-dimensional variable turn (3DVT) model, an adaptive tracking algorithm is proposed by combining with the cubature Kalman filter (CKF) in this paper. Through ideology of real-time identification, the parameters of the model are changed to adjust the state transition matrix and the state noise covariance matrix. Therefore, states of the target are matched in real-time to achieve the purpose of adaptive tracking. Finally, four simulations are analyzed in different settings by the Monte Carlo method. All results show that the proposed algorithm can update parameters of the model and identify motion characteristics in real-time when targets tracking also has a better tracking accuracy.展开更多
[ Objective] This study aimed to clone and identify enterocin gene from Enterococcus. [ Method] The genomic DNA of 12 enterocecci was extracted, and separately amplified with specific primers. The amplified fragments ...[ Objective] This study aimed to clone and identify enterocin gene from Enterococcus. [ Method] The genomic DNA of 12 enterocecci was extracted, and separately amplified with specific primers. The amplified fragments were ligated into PGEM-T Easy vector, which was then transformed into DH5α competent cells. The positive clones were sequenced. [Result] Enterocin A gene was 274 bp long. It was obtained from six enterococci, and the amino acids encoded by the enterocin genes cloned from five object enterocecci were the same as that of type IIa reference strains except only one amino acid. The homology among them reached 99.76 - 100%, suggesting that the bacteriocin isolated from the enterococcis belonged to type II. Structure prediction by DNAstar indicated that 22nd - 30th amino acids of enterocin A formed ot region, which had a hydrophilic region at its N-terminal and a hydrophobic region at its C-terminal, a transmembrane helix structure. [ Conclusion] This study will provide basis for the heterologous expression and applications of enterocins. Key words Enterocin gene; Enterocecci; PCR; Sequence analysis展开更多
基金supported by the National Natural Science Foundation of China(51467013)
文摘Satisfactory results cannot be obtained when three-dimensional (3D) targets with complex maneuvering characteristics are tracked by the commonly used two-dimensional coordinated turn (2DCT) model. To address the problem of 3D target tracking with strong maneuverability, on the basis of the modified three-dimensional variable turn (3DVT) model, an adaptive tracking algorithm is proposed by combining with the cubature Kalman filter (CKF) in this paper. Through ideology of real-time identification, the parameters of the model are changed to adjust the state transition matrix and the state noise covariance matrix. Therefore, states of the target are matched in real-time to achieve the purpose of adaptive tracking. Finally, four simulations are analyzed in different settings by the Monte Carlo method. All results show that the proposed algorithm can update parameters of the model and identify motion characteristics in real-time when targets tracking also has a better tracking accuracy.
基金Supported by General Project of Beijing Municipal Education Commissiona grant from the Schoolboard of Beijing,China(KM201110020005)
文摘[ Objective] This study aimed to clone and identify enterocin gene from Enterococcus. [ Method] The genomic DNA of 12 enterocecci was extracted, and separately amplified with specific primers. The amplified fragments were ligated into PGEM-T Easy vector, which was then transformed into DH5α competent cells. The positive clones were sequenced. [Result] Enterocin A gene was 274 bp long. It was obtained from six enterococci, and the amino acids encoded by the enterocin genes cloned from five object enterocecci were the same as that of type IIa reference strains except only one amino acid. The homology among them reached 99.76 - 100%, suggesting that the bacteriocin isolated from the enterococcis belonged to type II. Structure prediction by DNAstar indicated that 22nd - 30th amino acids of enterocin A formed ot region, which had a hydrophilic region at its N-terminal and a hydrophobic region at its C-terminal, a transmembrane helix structure. [ Conclusion] This study will provide basis for the heterologous expression and applications of enterocins. Key words Enterocin gene; Enterocecci; PCR; Sequence analysis
