Development of Laser Fusion Printing Machine for Ceramic Teeth Crown

Since former president Obama of America put forward the concept of 3D printing or additive manufacturing, it had been putting into use rapidly and getting acceptance widely. In particular, metal additive manufacturing machines had been successfully applied with pilot demonstration in industry. However, the present metal additive manufacturing machines cannot be directly used in medical fields such as dental restoration because of some different requirements between industry and medical fields. In this case, this paper is aimed for the development of laser fusion printing machine (LFP), also being called as selective laser melting (SLM), for ceramic teeth crown in dental restoration business. Through the reasonable design and development of key components such as machinery unit, optical unit, electrical controlling unit, and software unit, and the integration, debugging, and optimization of the entire system, the laser fusion printing apparatus for dental restoration has been successfully developed. Key technologies such as machine structure design, optical unit design, electrical controlling system design, system software and process software have been overcome, on the basis of which, a lot of process experiments of medical titanium alloy materials were deeply carried out. At last laser fusion printing technology of titanium alloy was mastered, and titanium dental crown by laser fusion printing with relative density up to 97.37% was realized. After post treatment with porcelain, it was found that the laser fusion printed porcelain teeth with titanium alloy has good metal-ceramic bonding strength, which is equivalent to the quality of traditional porcelain teeth, which showed that laser fusion printing can meet the requirements of dental restoration business and has a broad market outlook.

Cloning,expression and activity analysises of chalcone synthase genes in Carthamus tinctorius

Objective:Flavonoids are the bioactive compounds in safflower(Carthamus tinctorius),in which chalcone synthase(CHS)is the first limiting enzyme.However,it is unclear that which chalcone synthase genes(CHSs)are participated in flavonoids biosynthesis in C.tinctorius.In this study,the CHSs in the molecular characterization and enzyme activities were investigated.Methods:Putative chalcone biosynthase genes were screened by the full-length transcriptome sequences data in C.tinctorius.Chalcone biosynthase genes in C.tinctorius(CtCHSs)were cloned from cDNA of flowers of C.tinctorius.The cloned gene sequences were analyzed by bioinformatics,and their expression patterns were analyzed by real-time PCR(RT-PCR).The protein of CtCHS in the development of flowers was detected by polyclonal antibody Western blot.A recombinant vector of CtCHS was constructed.The CtCHS recombinant protein was induced and purified to detect the enzyme reaction(catalyzing the reaction of p-coumaryl-CoA and malonyl-CoA to produce naringin chalcone).The reaction product was detected by HPLC and LC-MS.Results:Two full-length CtCHS genes were successfully cloned from the flowers of safflower(CtCHS1 and CtCHS3),with gene lengths of 1525 bp and 1358 bp,respectively.RT-PCR analysis showed that both genes were highly expressed in the flowers,but the expression of CtCHS1 was higher than that of CtCHS3 at each developmental stage of the flowers.WB analysis showed that only CtCHS1 protein could be detected at each developmental stage of the flowers.HPLC and LC-MS analyses showed that CtCHS1 could catalyze the conversion of p-coumaryl-CoA and malonyl-CoA substrates to naringin chalcone.Conclusion:CtCHS1 is involved in the biosynthesis of naringin chalcone in safflower.

Fast Zn^(2+)mobility enabled by sucrose modified Zn^(2+)solvation structure for dendrite-free aqueous zinc battery

Aqueous zinc battery has been regarded as one of the most promising energy storage systems due to its low cost and environmental benignity.However,the safety concern on Zn anodes caused by uncontrolled Zn dendrite growth in aqueous electrolyte hinders their application.Herein,sucrose with multi-hydroxyl groups has been introduced into aqueous electrolyte to modify Zn^(2+)solvation environment and create a protection layer on Zn anode,thus effectively retarding the growth of zinc dendrites.Atomistic simulations and experiments confirm that sucrose molecules can enter into the solvation sheath of Zn^(2+),and the as-formed unique solvation structure enhances the mobility of Zn^(2+).Such fast Zn^(2+)kinetics in sucrose-modified electrolyte can successfully suppress the dendrite growth.With this sucrose-modified aqueous electrolyte,Zn/Zn symmetric cells present more stable cycle performance than those using pure aqueous electrolyte;Zn/C cells also deliver an impressive higher energy density of 129.7 Wh·kg^(−1)and improved stability,suggesting a great potential application of sucrose-modified electrolytes for future Zn batteries.

基于智能脉诊仪的“人与天地相参”研究

目的探讨脉象与"人与天地相参"理论的相关性及其重现性。方法于2012年7月22日(大暑)至2014年6月21日(夏至)、2016年10月23日(霜降)至2018年10月23日(霜降)分别进行二轮实验观察,第一轮观察对象42人,第二轮50人,均为中国中医科学院在职职工、在读研究生。基于动态标准化脉诊信息采集和分析技术,采集受试者双手寸、关、尺的脉诊信息,同时记录当日最高气温、最低气温、日出时间、日落时间、昼时长、夜时长天地背景数据。采用余弦函数谐波拟合脉图建模方法获取脉图参数,通过两轮次脉学实验观察,从脉图参数角度分析脉位、脉力与天地参数的相关性与重现性。结果在193个参数中,脉位、脉力是跟随天地参数(太阳角度、气温)变化最明显的两个参数。脉位、脉力在两轮次实验中均伴随太阳角度呈现规律性和重现性。两轮次实验的脉位、脉力与太阳角度具有强相关性或中等相关性。结论脉位、脉力呈现出"人与天地相参"的规律,且具有重现性。

沙棘籽油纳米乳的制备及经皮渗透的可视化分析研究（英文）

制备沙棘油纳米乳并对其理化性质和经皮渗透能力、皮肤刺激性进行考察。沙棘油纳米乳的最优处方为:以聚氧乙烯蓖麻油为乳化剂,二乙二醇单乙基醚为助乳化剂,肉豆蔻酸异丙酯为油相,肉豆蔻酸异丙酯:聚氧乙烯蓖麻油:沙棘油=5%:16%:20%, Km=4:1。制得外观澄清透明的淡黄色O/W型纳米乳,平均粒径为(52.2±4.8) nm,在透射镜下分布均一。经高速离心后,沙棘油纳米乳稳定性良好。测得沙棘油纳米乳的平均载药量为0.24mg·m L^-1 (RSD=1.47%),达10.68%。在激光共聚焦显微镜下,OB荧光标记的沙棘油纳米乳、纳米乳凝胶和肉豆蔻酸异丙酯溶液,透皮2小时后在表皮、真皮浅层和深层以及毛囊处的荧光强度均强于OB荧光标记的沙棘油和沙棘油乳膏。皮肤刺激性实验可知,制得的沙棘油纳米乳凝胶和沙棘油纳米乳在家兔正常和破损皮肤上均无明显可见的红肿现象。以上实验结果表明沙棘油纳米乳是一种潜在的用于皮肤治疗的传递系统。

基于BOTDA/R的在运光纤复合架空地线光缆应变监测对比分析

光纤复合架空地线(OPGW)故障频发的原因与光缆局部受到的应力有直接关系,为了实现对OPGW光缆的在线应变监测,搭建了长距离布里渊光时域分析(BOTDA)和布里渊光时域反射(BOTDR)系统,并对重覆冰区域运行年限超过15年的在运95.14 km OPGW进行了应变检测和分析。通过对同一段光缆的对比测量,结果表明:BOTDA系统具有更长的测量距离、更高的空间分辨率与更高的测量精度,能准确辨别引下线并识别零应变参考点,可以实现温度和应变信息的准确分离。在两个站点的距离超过BOTDA系统的测量量程时,可以使用BOTDR系统从光缆两端分别测量以覆盖全部光缆。同时,BOTDR系统展现出单端测量的优势,在断纤故障发生时,BOTDR系统可不影响断点之前的线路测量。在同一条OPGW光缆线路上通过多维度的对比,分析了两种技术在OPGW光缆监测中的优劣势,为分布式光纤传感技术在电力系统中的应用提供参考。

Enhanced deep-red emission in donor-acceptor molecular architecture:The role of ancillary acceptor of cyanophenyl

Organic solid-state luminescent materials with high-efficiency deep-red emission have attracted considerable interest in recent years.Constructing donor-acceptor(D-A)type molecules has been one of most commonly used strategies to achieve deep-red emission,but it is always difficult to achieve high photoluminescence(PL)quantum yield(ηPL)due to forbidden charge-transfer state.Herein,we report a new D-A type molecule 4-(7-(4-(diphenylamino)phenyl)-9-oxo-9H-fluoren-2-yl)benzonitrile(TPAFOCN),deriving from donor-acceptor-donor(D-A-D)type 2,7-bis(4-(diphenylamino)phenyl)-9H-fluoren-9-one(DTPA-FO)with a fluorescence maximum of 627 nm in solids.This molecular design enables a transformation of acceptor from fluorenone(FO)itself to 4-(9-oxo-9H-fluoren-2-yl)benzonitrile(FOCN).Compared with DTPA-FO,the introduction of cyanophenyl not only shifts the emission of TPA-FOCN to deep red with a fluorescence maximum of 668 nm in solids,but also maintains the highηPL of 10%.Additionally,a solution-processed non-doped organic light-emitting diode(OLED)was fabricated with TPA-FOCN as emitter.TPA-FOCN device showed a maximum luminous efficiency of 0.13 cd/A and a maximum external quantum efficiency(EQE)of 0.22%with CIE coordinates of(0.64,0.35).This work provides a valuable strategy for the rational design of high-efficiency deep-red emission materials using cyanophenyl as an ancillary acceptor.

Design of Photothermal Covalent Organic Frameworks by Radical Immobilization

Covalent organic frameworks(COFs)characterized by structural diversity,face-to-face stacking and open channels exhibit unique advantages as photothermal materials but have rarely been applied in solar-driven water evaporation due to complicated framework design,tedious synthesis,and low solar-to-vapor efficiency.Herein,we report a materials design strategy to produce efficient and robust photothermal COF by anchoring nonemissive radicals to the pore surface by a[2+2]cycloaddition–retroelectrocyclization reaction.