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拟南芥微管结合蛋白WDL3参与ABA诱导的气孔关闭过程
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作者 席小慧 王攀 +1 位作者 王朝阳 于荣 《科学通报》 EI CAS CSCD 北大核心 2019年第1期95-106,共12页
以拟南芥WDL3RNA干扰株系(WDL3RNAi)和Tubulin5A-YFP植株等为材料,从叶片的失水率、气孔开度、保卫细胞微管骨架动态排布以及Ca^(2+)流动等不同角度探究在脱落酸(abscisic acid, ABA)诱导的气孔关闭信号通路中,微管结合蛋白WDL3与微管... 以拟南芥WDL3RNA干扰株系(WDL3RNAi)和Tubulin5A-YFP植株等为材料,从叶片的失水率、气孔开度、保卫细胞微管骨架动态排布以及Ca^(2+)流动等不同角度探究在脱落酸(abscisic acid, ABA)诱导的气孔关闭信号通路中,微管结合蛋白WDL3与微管骨架以及Ca^(2+)之间的功能关系,深入了解气孔运动机理.结果表明:(1)相同条件下,WDL3RNAi的叶片蒸腾速率明显慢于野生型.(2)气孔开度实验中,WDL3RNAi对ABA信号比野生型更敏感,气孔关闭更快;微管稳定剂紫杉醇(Paclitaxel)可部分阻碍ABA的作用,微管解聚剂黄草消(Oryzalin)则进一步促进ABA诱导的气孔关闭,但WDL3 RNAi与野生型之间仍存在显著差异;激光共聚焦扫描显微镜观察发现, ABA条件下WDL3 RNAi保卫细胞内微管解聚明显加快,微管成束程度(bundling)显著降低.(3)胞内Ca^(2+)螯合剂BAPTA与ABA共同处理,野生型和WDL3RNAi的气孔关闭均受到不同程度的抑制,关闭减缓,处理前后差异显著.亚细胞结构观察发现, BAPTA阻碍了ABA引起的保卫细胞微管解聚,但WDL3 RNAi与野生型相比,依然维持相对较高的微管解聚比例.此外,非损伤微测技术检测发现,ABA引起的保卫细胞Ca^(2+)内流在WDL3RNAi中较野生型的流速更快,流量加大,显示Ca^(2+)在该信号通路中具有重要作用.综上实验结果表明,微管结合蛋白WDL3通过与微管骨架及Ca^(2+)相互作用参与ABA诱导的气孔关闭过程. 展开更多
关键词 WDL3 微管骨架 CA^2+ 气孔关闭 脱落酸
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On the Microstructural Strengthening and Toughening of Heat-Affected Zone in a Low-Carbon High-Strength Cu-Bearing Steel
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作者 xiaohui xi Jinliang Wang +1 位作者 Liqing Chen Zhaodong Wang 《Acta Metallurgica Sinica(English Letters)》 SCIE EI CAS CSCD 2021年第5期617-627,共11页
In this article,the influence of simulated thermal cycles for the heat-aff ected zone(HAZ)on the microstructural evolution and mechanical properties in a low-carbon high-strength Cu-bearing steel was investigated by m... In this article,the influence of simulated thermal cycles for the heat-aff ected zone(HAZ)on the microstructural evolution and mechanical properties in a low-carbon high-strength Cu-bearing steel was investigated by microstructural characterization and mechanical tests.The results showed that the microstructure of the coarse-grained heat-aff ected zone(CGHAZ)and the fine-grained heat-aff ected zone(FGHAZ)was mainly comprised of lath martensite,and a mixed microstructure consisting of intercritical ferrite,tempered martensite and retained austenite occurred in the intercritically heat-aff ected zone(ICHAZ)and the subcritically heat-aff ected zone(SCHAZ).Also,8–11%retained austenite and more or less Cu precipitates were observed in the simulated HAZs except for CGHAZ.Charpy impact test indicated that the optimum toughness was obtained in FGHAZ,which was not only associated with grain refinement,but also correlated with deformation-induced transformation of the retained austenite,variant confi guration as interleaved type and a relatively weak variant selection.The toughness of ICHAZ and SCHAZ exhibited a slight downtrend due to the presence of Cu precipitates.The CGHAZ has the lowest toughness in the simulated HAZs,which was attributed to grain coarsening and heavy variant selection.In addition,the contribution of Cu precipitates to yield strength in simulated HAZs was estimated based on Russell–Brown model.It demonstrated an inverse variation trend to toughness. 展开更多
关键词 Low-carbon high-strength steel Heat-affected zone Cu addition TOUGHNESS Precipitation strengthening
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